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    • Nā Pahu ʻIke Mpox Piha (RDT, NAAT a me ka Sequencing)

    Mai Mei 2022, ua hōʻike ʻia nā hihia MPOX ma nā ʻāina he nui ʻaʻole i hoʻopau ʻia ma ka honua me nā hoʻoili kaiāulu.

    Ma ka lā 26 o ʻAukake, ua hoʻokumu ka World Health Organization (WHO) i kahi ʻōlelo hoʻolaha honua noHoʻolālā Hoʻomākaukau a me ka Pane Hoʻolālāe hoʻōki i nā maʻi ahulau o ka laha ʻana o ka mpox mai ke kanaka a i ke kanaka ma o nā hana i hoʻonohonoho ʻia ma ka honua holoʻokoʻa, ʻāpana, a me ka ʻāina. Ma hope o ka hoʻolaha ʻana o kahi pilikia olakino lehulehu o ka hopohopo honua e ka Luna Hoʻokele Nui o WHO ma ka lā 14 o ʻAukake.

    Pono e hoʻomaopopo ʻia he ʻokoʻa ka maʻi ahulau mpox i kēia manawa mai ko ka makahiki 2022, kahi i laha nui i waena o nā kāne e moe kolohe ana me nā kāne, a ʻo ka nui o ka make ʻana o ka poʻe i loaʻa i ka maʻi he emi ma mua o 1%.

    ʻO ke ʻano laha hou ʻo "Clade Ib", he ʻano like ʻole ia o Clade I, ʻoi aku ka nui o ka make. Ua hoʻomaka kēia ʻano hou e laha ma DRC i Kepakemapa i hala, i ka wā mua ma waena o nā mea hana moekolohe, a ua laha aku i nā hui ʻē aʻe, me nā keiki i maʻalahi loa.

    Ua ʻōlelo ka ʻApelika CDC ma kahi hōʻike i ka mahina i hala aku nei ua loaʻa nā maʻi ahulau mpox ma 10 mau ʻāina ʻApelika i kēia makahiki, me DRC, nāna i hōʻike i 96.3% o nā hihia āpau ma ʻApelika i kēia makahiki a me 97% o nā make. He mea kūpono ke hoʻomaopopo ʻana he kokoke i 70% o nā hihia ma DRC he mau keiki ma lalo o 15 mau makahiki, a ʻo kēia hui ka helu no 85% o nā make ma ka ʻāina.

    He zoonosis ʻo Mpox i hoʻokumu ʻia e ka maʻi mpox me ka manawa hoʻoulu o 5 a 21 mau lā, ʻo ka hapa nui he 6 a 13 mau lā. E loaʻa i ka mea i loaʻa ka maʻi nā hōʻailona e like me ke kuni, ka ʻeha poʻo a me nā ʻōpū lymph pehu, a ukali ʻia e ka ʻulaʻula ma ka maka a me nā ʻāpana ʻē aʻe o ke kino, kahi e ulu mālie ai i loko o nā pustules a mau no hoʻokahi pule ma mua o ka ʻōpala. He laha ka hihia mai ka hoʻomaka ʻana o nā hōʻailona a hiki i ka hāʻule ʻana o nā ʻōpala ma ke ʻano maoli.

    Ke hāʻawi nei ʻo Macro & Micro-Test i nā hoʻokolohua wikiwiki, nā pahu molekala a me nā hoʻonā sequencing no ka ʻike ʻana i ka maʻi mpox, e kōkua ana i ka ʻike ʻana i ka maʻi mpox i ka manawa, ka nānā ʻana i kona kumu, ka moʻokūʻauhau, ka hoʻoili ʻana a me nā ʻano genomic:

    Antigen o ka maʻi MonkeypoxPahu ʻIke (ʻIkepili Immunochromatography)

    Laʻana maʻalahi (wai ʻulaʻula/laʻana ʻāʻī) a me ka hopena wikiwiki i loko o 10-15 min;

    ʻIke kiʻekiʻe me ka LoD o 20pg/mL e uhi ana iā Clade I & II;

    ʻO ke kūlana kiʻekiʻe me ka ʻole o ka cross-reactivity me ka maʻi smallpox, varicella zoster virus, rubella virus, herpes simplex virus, etc.

    ʻO OPA o 96.4% i hoʻohālikelike ʻia me nā NAAT;

    Hoʻohana ākea e like me nā dute, nā CDC, nā hale kūʻai lāʻau lapaʻau, nā keʻena lapaʻau, nā haukapila a i ʻole ma ka home.

    Pahu ʻike antibody IgM/IgG o ka maʻi Monkeypox(ʻIkepili Immunochromatography)

    Hoʻohana maʻalahi me ka ʻole o ka mea hana a me ka hopena wikiwiki i loko o 10 mau minuke;

    ʻO ke kiʻekiʻe o ka ʻike a me ke kikoʻī e uhi ana iā Clade I & II;

    Hoʻomaopopo i ka IgM a me IgG e hoʻoholo i nā pae o ka maʻi mpox;

    Hoʻohana ākea e like me nā dute, CDC, nā hale kūʻai lāʻau lapaʻau, nā keʻena lapaʻau, nā haukapila a i ʻole ma ka home;

    Kūpono no ka nānā nui ʻana i ka maʻi mpox i manaʻo ʻia.

    Pahu ʻIke Waikawa Nucleic Virus Monkeypox (Hoʻonui ʻia ʻana o ka Enzymatic Probe Isothermal)

    ʻO ke kiʻekiʻe o ka ʻike me LoD o 200 Copies/mL me IC, ua like ia me ka florescence PCR;

    Hana maʻalahi: Hoʻohui ʻia ka laʻana Lysed i ka paipu reagent lyophilized no ka hoʻonui pololei ʻana ma ke koi i hiki i nā modula kūʻokoʻa o Easy Amp System;

    ʻO ke kūlana kiʻekiʻe me ka ʻole o ka cross reactivity me ka maʻi smallpox, ka maʻi vaccinia, ka maʻi cowpox, ka maʻi mousepox, ka maʻi herpes simplex, ka maʻi varicella-zoster, a me ka genome kanaka, a pēlā aku.

    ʻO ka laʻana maʻalahi (ka wai ʻulaʻula/swab oropharyngeal) a me ka hopena maikaʻi loa i loko o 5 min;

    Hana maikaʻi loa i ka hana lapaʻau e uhi ana iā Clade I & II me PPA o 100%, NPA o 100%, OPA o 100% a me Kappa Value o 1.000 i hoʻohālikelike ʻia me ka pahu Fluorescence PCR;

    ʻO ka mana Lyophilized e koi wale ana i ka lawe ʻana a me ka mālama ʻana i ka mahana o ka lumi e hiki ai ke komo ma nā wahi āpau;

    Nā hiʻohiʻona maʻalahi i nā keʻena lapaʻau, ke kikowaena mālama ola, me Easy Amp no ka ʻike ʻana ma ke koi;

     

    Pahu ʻIke Waikawa Nucleic Virus Monkeypox (Fluorescence PCR) 

    ʻO ka gene pālua i hoʻopaʻa ʻia me ka ʻike kiʻekiʻe me LoD o 200 kope/mL;

    Laʻana maʻalahi o ka wai ʻulaʻula, ka ʻūpā ʻāʻī, a me ke serum;

    ʻO ke kūlana kiʻekiʻe me ka ʻole o ka cross reactivity me ka maʻi smallpox, ka maʻi vaccinia, ka maʻi cowpox, ka maʻi mousepox, ka maʻi herpes simplex, ka maʻi varicella-zoster, a me ka genome kanaka, a pēlā aku.

    Hana maʻalahi: wikiwiki ka lysis laʻana e ka reagent hoʻokuʻu laʻana e hoʻohui ʻia i ka ʻōmole hopena;

    ʻIke wikiwiki: hopena i loko o 40 min;

    ʻO ka pololei i hōʻoia ʻia e ka mana kūloko e nānā ana i ke kaʻina hana ʻike holoʻokoʻa;

    Hana maikaʻi loa i ka hana lapaʻau e uhi ana iā Clade I & II me PPA o 100%, NPA o 99.40%, OPA o 99.64% a me Kappa Value o 0.9923 i hoʻohālikelike ʻia me ka sequencing;

    ʻO ka mana Lyophilized e koi wale ana i ka lawe ʻana a me ka mālama ʻana i ka mahana o ka lumi e hiki ai ke komo ma nā wahi āpau;

    Kūlike me nā ʻōnaehana PCR Fluorescence mainstream;

    Nā hiʻohiʻona maʻalahi no nā haukapila, nā CDC a me nā keʻena hoʻokolohua;

     

    Pahu ʻike waikawa nucleic no ka maʻi Orthopox Universal Type/Monkeypox Virus (Fluorescence PCR)

    Uhi piha: hoʻāʻo i nā maʻi orthopox 4 āpau e hiki ke hoʻopili i ke kanaka a me ka mpox maʻamau (ua hoʻokomo ʻia ʻo Clade I&II) i ka hoʻāʻo hoʻokahi e pale aku i ka ʻike ʻole ʻia;

    ʻIke kiʻekiʻe me LoD o 200 kope/mL;

    ʻO ke kikoʻī kiʻekiʻe me ka ʻole o ka cross reactivity me nā pathogens ʻē aʻe e hoʻoulu ai i nā rashes e like me ka herpes simplex virus, varicella-zoster virus, a me ka genome kanaka, a pēlā aku;

    Hana maʻalahi: wikiwiki ka lysis laʻana e ka reagent hoʻokuʻu laʻana e hoʻohui ʻia i ka buffer reaction paipu hoʻokahi;

    ʻIke wikiwiki: hoʻonui wikiwiki me ka hopena i loko o 40 min;

    ʻO ka pololei i hōʻoia ʻia e ka mana kūloko e nānā ana i ke kaʻina hana ʻike holoʻokoʻa;

    Kūlike me nā ʻōnaehana PCR Fluorescence mainstream;

    Nā hiʻohiʻona maʻalahi no nā haukapila, nā CDC a me nā keʻena hoʻokolohua;

    MonkeypoxVʻIrus TypingNʻucleicAʻakikaDhoʻōla ʻanaKia (F(PCR luorescence)

    Hoʻomaopopo pū i ka Clade I a me Clade II, he mea nui ia no ka hoʻomaopopo ʻana i nā ʻano epidemiological o ka maʻi, ka hahai ʻana i kona laha ʻana, a me ka hoʻokumu ʻana i nā hana pale a me ka kaohi i kuhikuhi ʻia.

    ʻIke kiʻekiʻe me LoD o 200 kope/mL;

    Laʻana maʻalahi o ka wai ʻulaʻula, ka swab oropharyngeal a me ke serum;

    ʻO ke kikoʻī kiʻekiʻe me ka ʻole o ka reactivity cross ma waena o Clade I a me II, nā pathogens ʻē aʻe e hoʻoulu ai i nā rashes e like me ka herpes simplex virus, varicella-zoster virus, a me ka genome kanaka, a pēlā aku.

    Hana maʻalahi: wikiwiki ka lysis laʻana e ka reagent hoʻokuʻu laʻana e hoʻohui ʻia i ka buffer reaction paipu hoʻokahi;

    ʻIke wikiwiki: hopena i loko o 40 min;

    ʻO ka pololei i hōʻoia ʻia e ka mana kūloko e nānā ana i ke kaʻina hana ʻike holoʻokoʻa;

    ʻO ka mana Lyophilized e koi wale ana i ka lawe ʻana a me ka mālama ʻana i ka mahana o ka lumi e hiki ai ke komo ma nā wahi āpau;

    Kūlike me nā ʻōnaehana PCR Fluorescence mainstream;

    Nā hiʻohiʻona maʻalahi no nā haukapila, nā CDC a me nā keʻena hoʻokolohua;

    ʻO ka maʻi Monkey Universal Universal GenomeKa ʻike ʻanaPahu hana (NGS Multi-PCR)

    ʻO ka Monkeypox Virus Whole Genome Detection Kit hou i hoʻomohala ʻia e Macro & Micro-Test no nā kūlana like ʻole, i hui pū ʻia me ka ONT nanopore sequencer, hiki ke loaʻa i ka MPXV holoʻokoʻa genome me kahi uhi ʻaʻole i emi ma mua o 98% i loko o 8 mau hola. 

    Maʻalahi e hana: ʻenehana hoʻonui hoʻokahi-ʻanuʻu i hoʻopaʻa ʻia, hiki ke loaʻa ka moʻo genome holoʻokoʻa o ka maʻi mpox ma o ka hoʻonui hoʻokahi-pōʻai;

    ʻIke a pololei hoʻi: ʻike i nā laʻana haʻahaʻa a hiki i ka 32CT, a hiki i ka 600bp amplicon nanopore sequencing ke hoʻokō i ka ʻākoakoa genome kiʻekiʻe aʻe;

    Wikiwiki loa: Hiki i ka ONT ke hoʻopau i ka hōʻuluʻulu genome i loko o 6-8 mau hola;

    Hoʻohālikelike ākea: me ONT, Qi Carbon, SALUS Pro, lllumina, MGI a me nā mea nui ʻē aʻe 2nda me 3rdnā mea hoʻonohonoho hanauna.

    ʻIke loaʻO ka genome holoʻokoʻa o ka maʻi MonkeyKa ʻike ʻanaPahu hana-Illumina/MGI(NGS Multi-PCR)

    E pili ana i ka nui o nā 2 e kū neindnā mea hoʻonohonoho hanauna ma ka honua holoʻokoʻa, ua hoʻomohala pū ʻo Macro & Micro-Test i nā pahu ultra-sensitive e hoʻololi ana i nā mea hoʻonohonoho mainstream e hoʻokō i ka hoʻonohonoho genome viral laʻana haʻahaʻa.

    Hoʻonui pono: 1448 mau pālua o ka hoʻolālā primer ultra-dense 200bp amplicon no ka pono hoʻonui kiʻekiʻe a me ka uhi like ʻana;

    Hana maʻalahi: Hiki ke loaʻa ka waihona puke Mpox virus llumina/MGI ma o ka hoʻonui ʻana i ʻelua puni i loko o 4 mau hola, e pale ana i nā ʻanuʻu kūkulu hale waihona puke paʻakikī a me nā kumukūʻai reagent;

    ʻIke kiʻekiʻe: ʻike i nā laʻana haʻahaʻa a hiki i ka 35CT, e pale pono ana i nā hopena maikaʻi ʻole i hoʻokumu ʻia e ka hōʻino ʻana o ka ʻāpana a i ʻole ka helu kope haʻahaʻa;

    Hoʻohālikelike ākea me ka mainstream 2ndnā mea hoʻonohonoho hanauna e like me lllumina, Salus Pro a i ʻole MGI;A hiki i kēia manawa, ua hoʻopau ʻia ma mua o 400 mau hihia lapaʻau.

    Ka manawa hoʻouna: Aug-28-2024
    E kaomi iā enter e huli a i ʻole ESC e pani