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    • Nā Kiko ʻImi Mpox piha (RDT, NAAT a me ka Sequencing)

    Mai Mei 2022, ua hōʻike ʻia nā hihia mpox i nā ʻāina he nui ʻole i ka honua me ka hoʻouna ʻana i ke kaiāulu.

    Ma ka lā 26 ʻAukake, ua hoʻokumu ka World Health Organization (WHO) i kahi honuaHoʻomākaukau hoʻolālā a me ka hoʻolālā panee ho'ōki i ka hoʻolaha ʻana o ke kanaka-i-kanaka o ka mpox ma o ka hoʻoikaika ʻana i ka honua, kūloko, a me ka lāhui. Ma hope o ka hoʻolaha ʻana o kahi pilikia olakino olakino o ka hopohopo honua e ka Luna Hoʻokele Nui o WHO ma 14 ʻAukake.

    Pono e hoʻomaopopo ʻia he ʻokoʻa ka puka ʻana o ka mpox i kēia manawa mai ka makahiki 2022, ka mea i pālahalaha nui ʻia ma waena o nā kāne e moe ana me nā kāne, a ʻo ka nui o ka make o ka poʻe maʻi ma lalo o 1%.

    ʻO ka maʻi maʻamau "Clade Ib", kahi ʻano like ʻole o Clade I, ʻoi aku ka nui o ka make. Ua hoʻomaka kēia ʻano hou e laha ma DRC i ka mahina ʻo Kepakemapa i hala, ma waena o nā mea hana wahine, a ua hoʻolaha ʻia i kēia manawa i nā hui ʻē aʻe, me nā keiki i maʻalahi loa.

    Ua haʻi ka CDC CDC i kahi hōʻike i ka mahina i hala aku nei ua loaʻa nā mpox outbreaks ma 10 mau ʻāina ʻApelika i kēia makahiki, me DRC, ka mea i hōʻike i ka 96.3% o nā hihia āpau ma ʻApelika i kēia makahiki a me 97% o ka make. He mea pono e hoʻomaopopo ʻia ma kahi o 70% o nā hihia ma DRC he mau keiki ma lalo o nā makahiki 15, a ʻo kēia pūʻulu he 85% o ka make ma ka ʻāina.

    ʻO Mpox kahi zoonosis i hoʻokumu ʻia e ka maʻi mpox me ka manawa incubation o 5 a 21 mau lā, ʻo ka hapa nui o 6 a 13 mau lā. E loaʻa ana i ka maʻi nā hōʻailona e like me ke kuni, ke poʻo a me ka pehu o nā lymph nodes, a ukali ʻia e ka maʻi ma ka maka a me nā ʻāpana ʻē aʻe o ke kino, e ulu mālie a lilo i pustules a mau no hoʻokahi pule ma mua o ka scabbing. He maʻi lele ka hihia mai ka hoʻomaka ʻana o nā hōʻailona a hiki i ka hāʻule maoli ʻana o nā ʻōpala.

    Hāʻawi ʻo Macro & Micro-Test i nā hoʻokolohua wikiwiki, kits molecular a me nā hopena hoʻonā no ka ʻike ʻana i ka maʻi maʻi mpox, ke kōkua ʻana i ka maʻi maʻi mpox i ka manawa, ka nānā ʻana i kona kumu, ka hanauna, ka hoʻouna ʻana a me nā ʻano genomic:

    Monkeypox Virus AntigenKit ʻIke (Immunochromatography)

    ʻO ka laʻana maʻalahi (ka wai ʻāhuʻu / ʻāʻī) a me ka hopena wikiwiki i loko o 10-15 min;

    ʻIke kiʻekiʻe me ka LoD o 20pg/mL e uhi ana iā Clade I & II;

    Kiʻekiʻe kikoʻī me ka cross-reactivity me ka smallpox virus, varicella zoster virus, rubella virus, herpes simplex virus, etc.

    OPA o 96.4% i hoʻohālikelike ʻia me nā NAAT;

    Hoʻohana ākea e like me nā kuʻuna, nā CDC, nā lāʻau lapaʻau, nā haukapila, nā halemai a i ʻole ma ka home.

    Monkeypox-virus IgM/IgG Antibody Detection Kit(Immunochromatography)

    ʻO ka hana maʻalahi a me ka hopena wikiwiki i loko o 10 min;

    ʻO ka ʻike kiʻekiʻe a me ka kikoʻī e uhi ana iā Clade I & II;

    Hoʻomaopopo i ka IgM a me IgG e hoʻoholo i nā pae maʻi mpox;

    Hoʻohana ākea e like me nā kuʻuna, nā CDC, nā lāʻau lapaʻau, nā keʻena lapaʻau, nā haukapila a i ʻole ma ka home;

    He kūpono no ka nānā nui ʻana i ka maʻi mpox i manaʻo ʻia.

    ʻO ka pahu hoʻomaʻi ʻo Monkeypox Virus Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

    ʻIke kiʻekiʻe me LoD o 200 Kope/mL me IC, like me ka PCR florescence;

    Hana maʻalahi: Hoʻohui ʻia kahi laʻana Lysed i ka lyophilized reagent tube no ka hoʻonui pololei ʻana i ke koi i hiki i nā modula kūʻokoʻa o Easy Amp System;

    ʻO ke kūlana kiʻekiʻe me ka hoʻololi ʻole o ke keʻa me ka maʻi maʻi liʻiliʻi, ka maʻi vaccinia, ka maʻi cowpox, ka maʻi ʻiole, ka maʻi herpes simplex, ka maʻi varicella-zoster, a me ka genome kanaka, etc.;

    ʻO ka laʻana maʻalahi (ka wai ʻino/oropharyngeal swab) a me ka hopena maikaʻi loa i loko o 5 min;

    ʻO ka hana lapaʻau maikaʻi loa e uhi ana iā Clade I & II me PPA o 100%, NPA o 100%, OPA o 100% a me Kappa Value o 1.000 i hoʻohālikelike ʻia me Fluorescence PCR kit;

    ʻO ka mana Lyophilized e koi ana i ka lawe ʻana a me ka mālama ʻana i ka lumi e hiki ai ke komo i nā wahi āpau;

    ʻO nā hiʻohiʻona maʻalahi i nā keʻena lapaʻau, ke kikowaena mālama olakino, me ka Easy Amp no ka ʻike ʻana ma ke koi;

     

    ʻO ka pahu hoʻomaʻi ʻo Monkeypox Nucleic Acid Detection Kit (Fluorescence PCR) 

    ʻO nā ʻano gene ʻelua i hoʻopaʻa ʻia me ka ʻike kiʻekiʻe me LoD o 200 kope/mL;

    ʻO ka laʻana maʻalahi o ka wai ʻeha, ka ʻāʻī a me ka serum;

    ʻO ke kūlana kiʻekiʻe me ka hoʻololi ʻole o ke keʻa me ka maʻi maʻi liʻiliʻi, ka maʻi vaccinia, ka maʻi cowpox, ka maʻi ʻiole, ka maʻi herpes simplex, ka maʻi varicella-zoster, a me ka genome kanaka, etc.;

    ʻO ka hana maʻalahi: wikiwiki ka laʻana lysis e ka hāpana hoʻokuʻu reagent e hoʻohui ʻia i ka paipu hopena;

    ʻIke wikiwiki: hopena i loko o 40 min;

    Hoʻopaʻa pololei ʻia e ka mana kūloko e nānā ana i ke kaʻina ʻike holoʻokoʻa;

    ʻO ka hoʻokō lapaʻau maikaʻi loa e uhi ana iā Clade I & II me ka PPA o 100%, NPA o 99.40%, OPA o 99.64% a me ka waiwai Kappa o 0.9923 i hoʻohālikelike ʻia me ka sequencing;

    ʻO ka mana Lyophilized e koi ana i ka lawe ʻana a me ka mālama ʻana i ka lumi e hiki ai ke komo i nā wahi āpau;

    Kūpono me nā ʻōnaehana PCR Fluorescence mainstream;

    Nā hiʻohiʻona maʻalahi no nā haukapila, CDC a me nā labs;

     

    ʻO Orthopox Virus Universal Type/Monkeypox Virus Nucleic Acid Detection Kit (Fluorescence PCR)

    Hoʻopili piha: hoʻāʻo i nā maʻi maʻi orthopox 4 a pau i hiki ke hoʻopōʻino i ke kanaka a me ka mpox maʻamau (Clade I&II i hoʻokomo ʻia) i ka hoʻāʻo hoʻokahi e pale i ka ʻike ʻole ʻia;

    ʻIke kiʻekiʻe me LoD o 200 kope/mL;

    ʻO ka kikoʻī kiʻekiʻe me ka ʻole o ka hoʻoikaika ʻana me nā pathogens ʻē aʻe e hoʻoulu ai i nā ʻōpala e like me ka herpes simplex virus, varicella-zoster virus, a me ka genome kanaka, etc.;

    Hana maʻalahi: hoʻohui ʻia ka lysis wikiwiki ma ka hoʻokuʻu ʻana i ka reagent e hoʻohui ʻia i ka pahu hoʻoheheʻe hoʻokahi;

    ʻIke wikiwiki: hoʻonui wikiwiki me ka hopena i loko o 40 min;

    Hoʻopaʻa pololei ʻia e ka mana kūloko e nānā ana i ke kaʻina ʻike holoʻokoʻa;

    Kūpono me nā ʻōnaehana PCR Fluorescence mainstream;

    Nā hiʻohiʻona maʻalahi no nā haukapila, CDC a me nā labs;

    MonkeypoxVirus TypingNucleicAcidDetectionKia (Fluorescence PCR)

    Hoʻomaopopo like i ka Clade I a me Clade II, he mea nui ia no ka hoʻomaopopo ʻana i nā hiʻohiʻona epidemiological o ka maʻi maʻi, ka ʻimi ʻana i kāna hoʻouna ʻana, a me ka hoʻokumu ʻana i nā hana pale a me nā kaohi.

    ʻIke kiʻekiʻe me LoD o 200 kope/mL;

    ʻO ka hoʻoheheʻe ʻana o ka wai o ka oropharyngeal a me ka serum;

    ʻO ke kūlana kiʻekiʻe me ka ʻole o ka hoʻoulu ʻana ma waena o Clade I a me II, nā pathogens ʻē aʻe e hoʻoulu ai i nā ʻōpala e like me ka herpes simplex virus, varicella-zoster virus, a me ka genome kanaka, etc.;

    Hana maʻalahi: hoʻohui ʻia ka lysis wikiwiki ma ka hoʻokuʻu ʻana i ka reagent e hoʻohui ʻia i ka pahu hoʻoheheʻe hoʻokahi;

    ʻIke wikiwiki: hopena i loko o 40 min;

    Hoʻopaʻa pololei ʻia e ka mana kūloko e nānā ana i ke kaʻina ʻike holoʻokoʻa;

    ʻO ka mana Lyophilized e koi ana i ka lawe ʻana a me ka mālama ʻana i ka lumi e hiki ai ke komo i nā wahi āpau;

    Kūpono me nā ʻōnaehana PCR Fluorescence mainstream;

    Nā hiʻohiʻona maʻalahi no nā haukapila, CDC a me nā labs;

    Monkey Virus Universal Whole GenomeʻIke ʻanaKit (Mul-PCR NGS)

    ʻO ka Monkeypox Virus Whole Genome Detection Kit i hoʻomohala hou ʻia e Macro & Micro-Test no nā hiʻohiʻona like ʻole, i hui pū ʻia me ka ONT nanopore sequencer, hiki ke loaʻa i ka MPXV holoʻokoʻa genome sequence me kahi uhi ʻaʻole emi ma lalo o 98% i loko o 8 mau hola. 

    E maʻalahi ka hana: ʻenehana hoʻonui hoʻokahi ʻanuʻu i hoʻopaʻa ʻia, hiki ke loaʻa ke kaʻina genome holoʻokoʻa o ka maʻi mpox ma o ka hoʻonui ʻana i hoʻokahi puni;

    ʻIke a pololei: ʻike i nā laʻana haʻahaʻa a hiki i 32CT, a hiki i ka 600bp amplicon nanopore sequencing ke hālāwai me ka hui genome kiʻekiʻe;

    Ka wikiwiki loa: Hiki i ka ONT ke hoʻopau i ka hui genome i loko o 6-8 mau hola;

    Hoʻohālikelike ākea: me ONT, Qi Carbon, SALUS Pro, lllumina, MGI a me nā mea nui 2nda me 3rdhanauna sequencers.

    ʻAiʻeleʻeleMonkey Virus Whole GenomeʻIke ʻanaKit-Illumina/MGI(Mul-PCR NGS)

    E pili ana i nā helu nui o ka 2 i kēia manawandnā mea hoʻonohonoho hanauna ma ka honua holoʻokoʻa, ua hoʻomohala pū ʻo Macro & Micro-Test i nā kit ultra-sensitive e hoʻopili ana i nā sequencers mainstream e hoʻokō i ka laʻana haʻahaʻa haʻahaʻa viral genome sequencing;

    Hoʻonui maikaʻi: 1448 mau pālua o 200bp amplicon ultra-dense primer hoʻolālā no ka hana hoʻonui kiʻekiʻe a me ka uhi like ʻole;

    ʻO ka hana maʻalahi: Hiki ke loaʻa ka waihona Mpox virus llumina/MGI ma o ka hoʻonui ʻana i ʻelua mau hola i loko o 4 mau hola, e pale ana i nā hana kūkulu hale waihona puke paʻakikī a me nā kumukūʻai reagent;

    ʻIke kiʻekiʻe: ʻike i nā laʻana haʻahaʻa a hiki i 35CT, e pale pono i nā hopena maikaʻi ʻole i hoʻokumu ʻia e ka hōʻemi ʻana a i ʻole ka helu kope haʻahaʻa;

    Hoʻohālikelike ākea me ka mainstream 2ndnā mea hoʻonohonoho hanauna e like me lllumina, Salus Pro a i ʻole MGI;A hiki i kēia manawa, ʻoi aku ma mua o 400 mau hihia lapaʻau i hoʻopau ʻia.

    Ka manawa hoʻouna: ʻAukake-28-2024
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