EasyAmp e ka Macro & Micro Test—-He Mea Hana Hoʻonui Isothermal Fluorescence Amplification i kūpono me LAMP/RPA/NASBA/HDA

Hana maikaʻi a me ka hoʻohana ākea

Easy Amp, e ka ʻenehana o isothermal nucleic acid amplificationhōʻike ʻia me ka kiʻekiʻeka naʻau a me kapōkolepane ʻanamanawa me ke koi ole no kaʻina hoʻololi wela. No laila, ua loaapuka maiede like me kanui loaʻenehana punahele no ka hoʻāʻo ʻana i ka waika nucleic wikiwiki.Me ka pilikia ole tʻōnaehana kaʻa kaʻa hermal, kahi mea hoʻonui isothermal maʻalahihiki ke hooluolu i kaholoʻokoʻa kaʻina hana.

Ua hoʻomohala kūʻokoʻa ka Macro & Micro-Testa hoʻokuʻu ʻiaka EasyAmp,he mea hana hoʻonui isothermal fluorescentno nā noi like ʻole.

I kēia manawa, ʻo kaAmp maʻalahi ua hōʻoia ʻia e lilokūpono me nā ʻenehana hoʻonui isothermal: Loop-Mediated Isothermal Amplification (LAMP), Recombinase Polymerase Amplification (RPA/RAA/MIRA), Nucleic Acid Sequence Based Amplification (NASBA), a me Helicase Dependent Amplification (HDA).He mea hana ʻoki ʻē aʻe ia no ka hoʻāʻo ʻana i ka waika nucleic wikiwiki me nā noi ākea.

· Loop-Mediated Isothermal Amplification (LAMP):Hoʻonui ma60-65°Cme ka pihapane ʻanakaʻina hanapau i loko o 30 minuke.E ike ana i ka Helu 1 no kona hoonui anakumukānāwai:

 

Fig 1. Kumuof LIMA 

· Recombinase Polymerase Amplification (RPA/RAA/MIRA):Hoʻonui ma37°C-42°Cme ka pihapane ʻanakaʻina hanapau i loko o 10-30 minuke.E ike ana i ka Helu 2 no konakumu hoʻonui:

Fig 2. Kumuof Hoʻonui ʻia ʻo Recombinase Polymerase

·Nucleic Acid Sequence Based Amplification (NASBA):Hoʻonui ma42°Cme ka pihapane ʻanakaʻina hanapau i loko o 40-120 minuke.E ike ana i ka Helu 3 no konakumu hoʻonui:

Fig 3. Kumuof Nucleic Acid Sequence Based Amplification

· Helicase Dependent Amplification (HDA):Hoʻonui ma65°Cme ka pihapane ʻanakaʻina hanapau i loko o 70-90 minuke.E ike ana i ka Helu 4 no konakumu hoʻonui:

Fig4.Kumukānāwaiof Hoʻonui ʻia i ka Helicase

· Ka EasyAmphikis hoʻonui maikaʻi a paʻa no ka ʻike DNA a me RNA me ka hoʻohana ʻana i nā ʻenehana isothermal ʻehā i ʻōlelo ʻia.Loaʻa iā ia ka hoʻonui ʻana a hiki i 200 kope / mL a hōʻoia i ka kikoʻī kiʻekiʻe me ka ʻole o ka cross-reactivity i ka hoʻonui ʻia..(Ihōʻike ʻia ma nā Kiʻi 5 a 7).

ʻIke DNA ʻIke RNA

Fig5.Pahu RAA/MIRA

ʻIke DNA ʻIke RNA

Fig6.LAMP Platform

 

ʻIke DNA ʻIke RNA

Fig7.Papahana NASBA

Nā Pono Huahana:

wikiwiki

· EPIAʻike ka ʻenehanahopena maikaʻiʻike ʻia i loko o 5 min.

· Hana maʻalahi me hoʻokahi-ʻanuʻu unuhino ka hoʻonui ʻana i ka mea kani.

Pono

· 4 mau modula kūʻokoʻa no ka noi, ka hoʻāʻo like ʻana o 4 ʻokoʻaʻano hoʻāʻos.

· Hiki i kēlā me kēia module ke kālailai ʻehā mau laʻana i ka manawa like.

· Pālua/Quadfluorescencenā kahawaino ka ʻike nui ʻana i nā pahuhopu lehulehu.

Hoʻolikelike

· Pili melehulehunā paepae isothermal e like me LAMP, RPA, NASBA, HDA.

· Hoʻolālā ʻia no ka hoʻonui ʻana i ka wela haʻahaʻa ma hope o ka transcription hoʻohuli.

· Customizhikie hoʻokō i nā pono o ka mea kūʻai aku.

Mea hoʻohana-aloha

· Phoʻolālā hoʻolālā(290×245×128mm, 2kg) no ka nā senarios noi like ʻole.

· 7-ʻīniha waihoʻoluʻu touchscreenno ka hana maʻalahi a me ke kiʻi ʻike hopena.

· Paʻi wela i kūkulu ʻia no ka paʻi ʻana i ka hopena koke.

· Hoʻopili ʻia ka scanner no ka heluhelu ʻana i nā mea.