Ka Loiloi o ka HPV Genotyping ma ke ano he Diagnostic Biomarkers of Cervical Cancer Risk – On Applications of HPV Genotyping Detection

Loaʻa pinepine ka maʻi HPV i ka poʻe moe kolohe, akā ulu ka maʻi hoʻomau ma kahi hapa liʻiliʻi o nā hihia. ʻO ka hoʻomau ʻana o ka HPV e pili ana i kahi pilikia o ka hoʻomohala ʻana i nā maʻi cervix precancerous a, i ka hopena, ka maʻi maʻi cervical.

ʻAʻole hiki ke kanu ʻia nā HPVin vitroma nā ʻano hana maʻamau, a me ka ʻokoʻa kūlohelohe ākea o ka pane ʻana i ka immune humoral ma hope o ka maʻi i hoʻopilikia i ka hoʻohana ʻana i ka hoʻāʻo ʻana i ka antibody kikoʻī HPV i ka maʻi. No laila, loaʻa ka hōʻoia o ka maʻi HPV ma o ka hoʻāʻo ʻana i ka molekala, ʻo ka mea nui ma ka ʻike ʻana i ka genomic HPV DNA.

I kēia manawa, nui nā ʻano hana genotyping HPV kalepa. ʻO ke koho ʻana i ka mea kūpono e pili ana i ka hoʻohana i manaʻo ʻia, ʻo ia hoʻi: epidemiology, loiloi kano, a i ʻole nā ​​noiʻi lapaʻau.

No nā noiʻi epidemiological, hiki i nā ʻano hana genotyping HPV ke kaha kiʻi ʻana i ke ʻano prevalence kikoʻī.
No ka loiloi lāʻau lapaʻau, hāʻawi kēia mau hoʻāʻo i ka ʻikepili e pili ana i nā loli i ka laha ʻana o nā ʻano HPV ʻaʻole i hoʻokomo ʻia i loko o nā kano o kēia manawa, a hoʻomaʻamaʻa i ka hahai ʻana o nā maʻi hoʻomau.
No nā haʻawina lāʻau lapaʻau, ʻōlelo nā alakaʻi o ka honua i kēia manawa i ka hoʻohana ʻana i nā hoʻāʻo genotyping HPV ma waena o nā wahine 30 mau makahiki a keu aʻe me ka cytology maikaʻi ʻole a me nā hopena maikaʻi HPV HR, ma HPV-16 a me HPV-18 kūikawā. ʻO ka ʻike ʻana i ka HPV a me ka hoʻokae ʻana i nā genotypes kiʻekiʻe a me ka haʻahaʻa haʻahaʻa i ʻelua a ʻoi aʻe paha e ʻike i nā maʻi me nā maʻi hoʻomau like genotype, e hopena i ka hoʻokele olakino maikaʻi.

Macro & Micro-Test HPV kit genotyping:

• 14 Nā ʻano HPV (Genotyping) Nucleic Acid Detection Kit (Fluorescence PCR)
• He 14 mau ʻano HPV (Genotyping) i hoʻomaloʻo paʻa ʻia i ka pahu Nucleic Acid Detection Kit(Fluorescence PCR)
• 28 ʻAno HPV (Genotyping) ʻIke ʻIke (Fluorescence PCR)(18 HR-HPV +10 LR-HPV)
• 28 mau ʻano HPV (Genotyping) i hoʻomaloʻo ʻia i ka pahu (Fluorescence PCR)

Nā hiʻohiʻona huahana nui:

• ʻIke like ʻana o nā genotypes he nui i hoʻokahi pane;
• ʻO ka manawa hoʻololi PCR pōkole no ka hoʻoholo wikiwiki;
• ʻO nā ʻano laʻana hou aʻe (mimi / swab) no ka nānā ʻana i ka maʻi HPV ʻoluʻolu a hiki ke loaʻa;
• Kāohi ʻo Dual Internal Controls i ka hoʻopunipuni a hōʻoia i ka hilinaʻi hoʻāʻo;
• Nā ʻano wai a me nā lyophilized no nā koho o nā mea kūʻai aku;
• Hoʻohālikelike me ka hapa nui o nā ʻōnaehana PCR no ka hoʻololi ʻana i ka lab.