ʻO ka pahu ʻike waikawa nucleic Streptococcus Group B (Enzymatic Probe Isothermal Amplification)
1. Ke koʻikoʻi o ka ʻike ʻana
ʻO ka maʻamau, noho ka streptococcus Group B (GBS) i loko o ka vagina a me ka rectum o nā wahine, hiki ke alakaʻi i ka maʻi invasive mua (GBS-EOS) i nā pēpē hānau hou ma o ka hoʻoili ʻana mai ka makuahine a i ke keiki, a ʻo ia ke kumu nui o ka pneumonia neonatal, meningitis, septicemia a me ka make. I ka makahiki 2021, ua hōʻike ka Expert Consensus on Clinical Management of High-risk Newborns with Early-onset Infection in the Same Room of Mothers and Infants of China Maternal and Child Health Association he mau hana kūpono ka nānā ʻana o GBS 35-37 mau pule ma mua o ka hānau ʻana a me ka pale ʻana i ka antibiotic intrapartum (IAP) e pale aku ai iā GBS-EOS i nā pēpē hānau hou.
2. Nā pilikia i kū i nā ʻano hana ʻike o kēia manawa
ʻO ka haki mua ʻana o nā membrane (PROM) e pili ana i ka haki ʻana o nā membrane ma mua o ka hānau ʻana, he pilikia maʻamau ia i ka wā perinatal. ʻO ka haki mua ʻana o nā membrane Ma muli o ka haki ʻana o nā membrane, ʻoi aku ka nui o ka laha ʻana o GBS i loko o ka vagina o nā wahine hānau, e hopena ana i ka maʻi intrauterine. ʻO ka pilikia o ka maʻi e pili pono ana i ka ulu ʻana i ka manawa o ka haki ʻana o nā membrane (> 50% o nā wahine hāpai e hānau i loko o 1-2h mau hola ma hope o ka haki ʻana o nā membrane, a i ʻole 1-2 mau hola).
ʻAʻole hiki i nā ʻano hana ʻike i kēia manawa ke hoʻokō piha i nā koi o ka manawa kūpono (< 1h), ka pololei a me ka ʻike ʻana o GBS ma ke kelepona i ka wā e hāʻawi ʻia ai.
| Nā mea hana ʻike | Moʻomeheu bacteria | Ka manawa moʻomeheu: 18-24hInā ʻo ka hoʻāʻo ʻana i ka ʻike o ka lāʻau lapaʻau: hoʻonui i ka 8-16h | 60% ka nui o ka ʻike ʻana; i ka wā o ke kaʻina hana laʻana, hiki ke maʻalahi i nā bacteria e like me Enterococcus faecalis a puni ka vagina a me ka anus, e hopena ana i nā hopena maikaʻi ʻole / maikaʻi ʻole. |
| ʻIkepili Immunochromatography | Ka manawa ʻike: 15min. | Haʻahaʻa ka ʻike, a maʻalahi ke hala ʻole ka ʻike ʻana, ʻoiai inā he liʻiliʻi ka nui o ka bacteria, paʻakikī ke ʻike, a ʻaʻole i ʻōlelo nui ʻia nā alakaʻi. | |
| PCR | Ka manawa ʻike: 2-3h | ʻOi aku ka manawa ʻike ma mua o 2 mau hola, a pono e hoʻāʻo ʻia ka mea hana PCR ma nā ʻāpana, a ʻaʻole hiki ke hahai i ka hoʻāʻo. |
3. Nā mea koʻikoʻi o ka huahana Macro & Micro-Test
ʻIke wikiwiki: Me ka hoʻohana ʻana i ke ʻano hoʻonui mahana mau o ka ʻōnaehana hoʻoheheʻe enzyme i hoʻopaʻa ʻia, hiki i nā mea maʻi maikaʻi ke ʻike i ka hopena i ka wā e 5 mau minuke.
ʻIke ʻia i kēlā me kēia manawa, ʻaʻohe pono e kali: ua lako pū me kahi mea loiloi hoʻonui waikawa nucleic mahana mau Easy Amp, a holo kūʻokoʻa nā modula ʻehā, a nānā ʻia nā laʻana i ko lākou hiki ʻana mai, no laila ʻaʻohe pono e kali no nā laʻana.
ʻAno Multi-laʻana: hiki ke ʻike ʻia ka swab vaginal, ka swab rectal a i ʻole ka swab vaginal i hui pū ʻia, kahi e hoʻokō ai i nā ʻōlelo aʻoaʻo o nā alakaʻi GBS, hoʻomaikaʻi i ka helu ʻike maikaʻi a hoʻemi i ka helu kuhi hewa.
Hana maikaʻi loa: hōʻoia lapaʻau laʻana nui multi-center (> 1000 mau hihia), ʻike 100%, kikoʻī 100%.
ʻO ka reagent hāmama: kūlike me ka mea hana PCR fluorescence quantitative mainstream o kēia manawa.
4. ʻIke huahana
| Helu Huahana | Inoa Huahana | Nā kikoʻī | Helu palapala hōʻoia hoʻopaʻa inoa |
| HWTS-UR033C | Pahu ʻike waikawa nucleic Streptococcus Group B (Hoʻonui ʻia ʻana o ka Enzymatic Probe Isothermal) | 50 mau hoʻāʻo/kit | Kakau Inoa Mīkini Kina 20243400248 |
| HWTS-EQ008 | ʻŌnaehana ʻIke Isothermal Fluorescence manawa maoli maʻalahi Amp | HWTS-1600P (4-kahawai) | Kakau Inoa Mīkini Kina 20233222059 |
| HWTS-1600S（2-kahawai） | |||
| HWTS-EQ009 |
Ka manawa hoʻouna: Malaki-07-2024