14 Nā ʻano maʻi hanu i hui ʻia
inoa huahana
HWTS-RT159B 14 mau ʻano o ka hanu ʻana i hui pū ʻia me ka ʻakika Nucleic Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi hanu ka maʻi maʻamau i nā kānaka, hiki ke loaʻa i kēlā me kēia kāne, makahiki a me ka ʻāina. ʻO ia kekahi o nā kumu nui o ka maʻi a me ka make i ka heluna kanaka o ka honua[1]. ʻO nā maʻi maʻi hanu maʻamau e pili ana i ka coronavirus novel, ka maʻi Influenza A, ka maʻi Influenza B, ka maʻi syncytial Respiratory, Adenovirus, metapneumovirus kanaka, rhinovirus, Parainfluenza maʻi ʻano I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, a me Streptococcus pneumoniae, etc.[2,3].
Kanal
| Kūlana maikaʻi | Inoa Haʻina Hana | E ʻike ʻia nā maʻi maʻi |
| 1 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 2 | Kumu Mix B | Adv, hMPV, MP, Cpn |
| 3 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, ka mana o loko |
| 5 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 6 | Kumu Mix B | Adv, hMPV, MP, Cpn |
| 7 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, ka mana o loko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | Oropharyngeal swab、Nasopharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Kope/mL |
| kikoo | Ua hōʻike ʻia nā hopena hoʻāʻo cross-reactivity ʻaʻohe hopena keʻa ma waena o kēia kit a me Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophilalisted strains. Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Corstriatum, Corstriatum, Corstriatum, No. Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii a me ke kanaka genomic nuclei. |
| Nā mea hana pili | SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.)Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻokele Thermal Cycler Manawa maoli (Suzhou Molarray Co., Ltd.) ʻO BioRad CFX96 Pūnaehana PCR manawa maoli, BioRad CFX Opus 96 Pūnaehana PCR manawa maoli. |
Holo Hana
Manaʻo ʻia ka hoʻoulu ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hana ʻia nā hana ma hope e like me nā ʻōlelo aʻoaʻo no ka hoʻohana ʻana i kēia reagent extraction. ʻO ka nui elution i manaʻo ʻia80µL.
