15 ʻAno o ka Human Papillomavirus E6/E7 Gene mRNA kiʻekiʻe-pilikia
Inoa huahana
HWTS-CC005A-15 Nā ʻAno o ke Kinohi Kiʻekiʻe o ka Human Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
ʻAlapia
ʻO ke kanesa ʻōpū kekahi o nā ʻano maʻamau o ke kanesa wahine ma ka honua holoʻokoʻa, a pili loa kona hanana ʻana i nā human papillomaviruses (HPV), akā he hapa liʻiliʻi wale nō o nā maʻi HPV e hiki ke ulu i ke kanesa. Hoʻopilikia ka HPV kiʻekiʻe i nā cell epithelial cervical a hana i ʻelua oncoproteins, ʻo E6 a me E7. Hiki i kēia protein ke hoʻopilikia i nā ʻano protein cellular like ʻole (e like me nā protein suppressor tumor pRB a me p53), hoʻolōʻihi i ke kaʻina hana cell, hoʻopilikia i ka synthesis DNA a me ke kūpaʻa genome, a hoʻopilikia i nā pane pale antiviral a me antitumor.
Kanal
| Kanal | ʻĀpana | Ua hoʻāʻo ʻia ka Genotype |
| ʻOhana | Palekana Hoʻihoʻi HPV 1 | HPV16,31,33,35,51,52,58 |
| VIC/HEX | ʻO ke kine β-actin kanaka | |
| ʻOhana | Hoʻopaʻa Hana HPV 2 | HPV 18,39,45,53,56,59,66,68 |
| VIC/HEX | ʻO ke kini INS kanaka |
Nā Palena ʻenehana
| Ka Waihona | Wai: ≤-18 ℃ |
| Ola papa | 9 mahina |
| ʻAno Laʻana | ʻŌpala cervical |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Kope/mL |
| Nā Mea Hana Pili | ʻŌnaehana PCR Manawa Maoli Biosystems 7500 i hoʻopili ʻiaNā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli QuantStudio®5 Nā ʻōnaehana PCR Manawa Maoli SLAN-96P ʻŌnaehana PCR Manawa Maoli LightCycler®480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
ʻO ka mea hoʻohuihui i ʻōlelo ʻia: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hana pololei ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka elution i ʻōlelo ʻia he 50μL. Inā ʻaʻole i ʻeli piha ʻia ka hāpana, e hoʻihoʻi iā ia i ka pae 4 no ka ʻeli hou ʻana. A laila e hoʻāʻo e like me nā kuhikuhi no ka hoʻohana ʻana.
ʻO ka mea hoʻohuihui i ʻōlelo ʻia: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Pono e hana ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana pono ʻana (Ma ke kaʻina hana 5, e pāpālua i ka nui o ka hopena hana DNaseI, ʻo ia hoʻi, e lawe i 20μL o ka hopena waihona RNase-Free DNaseI (1500U) i loko o kahi paipu centrifuge RNase-Free hou, e hoʻohui i 60μL o ka buffer RDD, a kāwili mālie). ʻO ka nui o ka elution i ʻōlelo ʻia he 60μL. Inā ʻaʻole i ʻeli piha ʻia ka hāpana, e hoʻihoʻi iā ia i ke kaʻina hana 5 no ka ʻeli hou ʻana. A laila e hoʻāʻo e like me nā kuhikuhi no ka hoʻohana ʻana.
