15 Nā ʻano o ke kanaka Papillomavirus E6/E7 Gene mRNA
Inoa mea kūʻai
HWTS-CC005A-15 Nā ʻano o ke kanaka Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi ʻaʻai ʻāʻī kekahi o nā ʻano maʻi maʻi maʻamau o ka wahine ma ka honua holoʻokoʻa, a ua pili loa kona hanana ʻana me nā papillomavirus kanaka (HPV), akā hiki i kahi hapa liʻiliʻi o nā maʻi HPV ke ulu i ka maʻi kanesa.Hoʻopilikia ka HPV i ka maʻi epithelial cervical a hoʻopuka i ʻelua oncoproteins, E6 a me E7.Hiki i kēia pūmua ke hoʻopili i nā ʻano protein cellular like ʻole (e like me ka tumor suppressor proteins pRB a me p53), hoʻolōʻihi i ke kaʻina cell, pili i ka DNA synthesis a me ka genome stability, a keakea i nā pane antiviral a me antitumor immune.
Kanal
| Kanal | ʻāpana | Ua hoʻāʻo ʻia ka genotype |
| FAM | Hoʻopaʻa Paʻa HPV 1 | HPV16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Gene β-actin kanaka | |
| FAM | HPV Reaction Buffer 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Gene INS kanaka |
Nā ʻāpana ʻenehana
| Waihona | Ka wai: ≤-18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | ʻauʻau ʻāʻī |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 kope/mL |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoliHoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR Manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Manaʻo ʻia ka hoʻoneʻe ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e mālama pono ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana. .ʻO ka nui o ka elution i manaʻo ʻia he 50μL.Inā ʻaʻole ʻeli loa ka hāpana, e hoʻihoʻi i ka ʻanuʻu 4 no ka hoʻoheheʻe hou ʻana.A laila e ho'āʻo e like me nā kuhikuhi no ka hoʻohana.
Manaʻo ʻia ka mea hoʻohui: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Pono e hana ʻia ka unuhi ʻana e like me nā ʻōlelo aʻoaʻo no ka hoʻohana pono ʻana (Ma ka ʻanuʻu 5, pāpālua i ka neʻe ʻana o ka hopena hana DNaseI, ʻo ia hoʻi, e lawe i 20μL o RNase-Free DNaseI (1500U) stock solution i loko o kahi pahu centrifuge RNase-Free hou, hoʻohui i 60μL o RDD buffer, a hui mālie).ʻO ka nui o ka elution i manaʻo ʻia he 60μL.Inā ʻaʻole ʻeli loa ka hāpana, e hoʻihoʻi iā ia i ka ʻanuʻu 5 no ka hoʻoheheʻe hou ʻana.A laila e ho'āʻo e like me nā kuhikuhi no ka hoʻohana.
