15ʻO nāʻano o ka papilloavirus o ke kanaka kiʻekiʻe o ke kanaka kiʻekiʻe
Hua waihona
Hwts-cc005a-15ʻano o ke kanaka kiʻekiʻe o ka papillomavirus e6 / e7 van shancent spit (fluerescence pcr)
Epideminey
ʻO ka maʻi maʻi kolohe hoʻokahi o nāʻano maʻamau o ka maʻi maʻi maʻi maʻi maʻamau, aʻo kona hopena e pili nei i nā papilomavir man (HPV), akā he liʻiliʻi liʻiliʻi o nā maʻi HPV. ʻO nā mea kiʻekiʻe a HPV FORE FERFIT CERVICALIPEL EPITLILL E EPITHELIAL A E hana i nā OCCOProteins, E6 a me E7. Hiki i kēia protein ke pili i nāʻano protein cellular (e like me ka mea kūʻai aku i nā mea kūʻai aku a me ka p53)
Kauwe
| Kauwe | Hui | Ua ho'āʻoʻo Genotype |
| Ka ha | ʻO HPV RUSECACANAM 1 | HPV16,31,33,35,52,52,58 |
| VIC / Hex | Kanaka β-actin ge | |
| Ka ha | ʻO HPV RUSECACACACACACAS 2 | HPV 18,39,45,536,56,66,68 |
| VIC / Hex | Kanaka inty |
Nā poʻoaʻenehana
| Huanui | Wai: ≤-18 ℃ |
| 'Āmaʻomaʻo | 9 mau mahina |
| ʻAno kiko'ī | Crectical Swab |
| Ct | ≤38 |
| CV | <5.0% |
| Lolulu | 500 kope / ML |
| Nā mea pili | E noi i nā biosysteystems 7500 maoli o PCRNoi biosystems 7500 wikiwiki mau'ōnaehana PCR ʻO ka helu helu RETSTATIDO®5 i nā'ōnaehana PCR manawa SLAN-96P mea maoli maoli PCR ʻO Lightcycler®480 Reass-Time Systems PCR Linegene 9600 a me ka'ōnaehanaʻo PCR maoli Ma-6000 maoli-manawa nui ka manawa nui Biorad CFX96 Nā Kūlana Kūlana Lacr Biorad CFX OPus 96 Reass PCR System |
Nā kahe o ka hana
Hoʻonohonohoʻia ka uku uku . ^. ʻO ka nui o ka leo i'ōleloʻia he 50μl. Ināʻaʻole i hoʻokaʻawaleʻia ka hiʻohiʻona, e hoʻihoʻi iā ia i ka hana 4 no ka ulaula. A laila e ho'āʻo e like me nā'ōlelo no ka hoʻohanaʻana.
Hoʻonohonohoʻia ka uku houʻana: RNAPREP BIECE FACS MILSE TISSUE TISS RNA RENICTION SHIT (DP431). Pono e alakaʻiʻia ka extraction e like me nā'ōlelo aʻoaʻo no ka hoʻohana pololeiʻana (ma ka pae hana o ka mokuahi Dnasei (154L) E hoʻohui i 60μl o Rdd Buffer, a hui pū mālie). ʻO ka nui o ka leo i'ōleloʻia he 60μl. Ināʻaʻole i hoʻokaʻawaleʻia kaʻatikala, e hoʻihoʻi iā ia i ka hana 5 no ka ulaula. A laila e ho'āʻo e like me nā'ōlelo no ka hoʻohanaʻana.
