28 ʻAno o ka Human Papilloma Virus kiʻekiʻe (16/18 Typing) Nucleic Acid
Inoa huahana
HWTS-CC006A-28 Nā ʻAno o ka Human Papilloma Virus Kiʻekiʻe (16/18 Typing) Pahu ʻIke Waikawa Nucleic (Fluorescence PCR)
ʻAlapia
ʻO ke kanesa ʻōpū kekahi o nā puʻupuʻu ʻino maʻamau o ka ʻōnaehana hānau wahine. Ua hōʻike ʻia nā haʻawina ʻo nā maʻi mau o ka HPV a me nā maʻi he nui kekahi o nā kumu nui o ke kanesa ʻōpū. I kēia manawa, ʻaʻohe mau lāʻau lapaʻau kūpono i ʻike ʻia no ke kanesa ʻōpū i hoʻokumu ʻia e ka HPV, no laila ʻo ka ʻike mua ʻana a me ka pale ʻana i ka maʻi ʻōpū i hoʻokumu ʻia e ka HPV ke kī i ka pale ʻana i ke kanesa ʻōpū. He mea nui loa ka hoʻokumu ʻana i kahi hoʻāʻo diagnostic etiology maʻalahi, kikoʻī a wikiwiki hoʻi no ka ʻike lapaʻau a me ka mālama ʻana i ke kanesa ʻōpū.
Kanal
| Hoʻohuihui Hana | Kanal | ʻAno |
| PCR-Mix1 | ʻOhana | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Mana Kūloko | |
| PCR-Mix2 | ʻOhana | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | Wai: ≤-18 ℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | ʻUpena Cervical, ʻUpena Vaginal, Mimi |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300 Kope/mL |
| Nā Mea Hana Pili | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P LightCycler®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Mea hoʻohuihui i manaʻo ʻia: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E hoʻohui i 200μL o ka saline maʻamau e hoʻokuʻu hou i ka pellet ma ka hana 2.1, a laila e hana ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana i kēia mea hoʻohuihui. ʻO ka nui o ka elution i manaʻo ʻia he 80μL.
ʻO ka mea hoʻohuihui i ʻōlelo ʻia: QIAamp DNA Mini Kit (51304) a i ʻole Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). E hoʻohui i 200μL o ka saline maʻamau e hoʻokuʻu hou i ka pellet ma ka hana 2.1, a laila pono e hana ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana i kēia mea hoʻohuihui. ʻO ka nui o ka laʻana i unuhi ʻia o nā laʻana he 200μL a pau, a ʻo ka nui o ka elution i ʻōlelo ʻia he 100μL.
