28 Nā ʻano o ka maʻi maʻi papilloma kanaka kiʻekiʻe (16/18 paʻi) ʻakika nucleic
inoa huahana
HWTS-CC006A-28 Nā ʻano o ka maʻi maʻi papilloma kanaka kiʻekiʻe (16/18 paʻi ʻana) pahu ʻike ʻakika nucleic (Fluorescence PCR)
Epidemiology
ʻO ka maʻi ʻaʻai ʻāʻī kekahi o nā maʻi maʻi maʻamau o ka ʻāpana hānau wahine. Ua hōʻike ʻia nā haʻawina ʻo nā maʻi hoʻomau HPV a me nā maʻi he nui kekahi o nā kumu nui o ka maʻi kanesa cervical. I kēia manawa, ʻaʻole i ʻike ʻia nā lāʻau lapaʻau maikaʻi no ka maʻi maʻi ʻāʻī ma muli o ka HPV, no laila, ʻo ka ʻike mua ʻana a me ka pale ʻana i ka maʻi ʻāʻī i hoʻokumu ʻia e ka HPV ʻo ia ke kī i ka pale ʻana i ka maʻi maʻi cervical. He mea koʻikoʻi ka hoʻokumu ʻana i kahi hōʻike diagnostic etiology maʻalahi, kikoʻī a wikiwiki hoʻi no ka maʻi maʻi a me ka mālama ʻana i ka maʻi maʻi cervical.
Kanal
| Hui Hana | Kanal | ʻAno |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Hoomalu Kuloko | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | Ka wai: ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | ʻO ka ʻauʻau ʻāʻī, ka ʻāwīwīwī, ka mimi |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300 kope/mL |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Manaʻo ʻia ka hoʻoneʻe ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (000HWC Acid Extractor). HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Hoʻohui i 200μL o ka paʻakai maʻamau e hoʻokuʻu hou i ka pellet ma ka pae 2.1, a laila pono e hana ʻia ka unuhi e like me nā kuhikuhi no ka hoʻohana ʻana i kēia reagent extraction. ʻO ka nui o ka elution i manaʻo ʻia he 80μL.
Manaʻo ʻia ka mea hoʻoheheʻe: QIAamp DNA Mini Kit (51304) a i ʻole Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). E hoʻohui i 200μL o ka paʻakai maʻamau e hoʻokuʻu hou i ka pellet ma ka pae 2.1, a laila pono e hoʻokō ʻia ka unuhi e like me nā kuhikuhi no ka hoʻohana ʻana i kēia reagent. He 200μL ka nui o nā laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 100μL.
