ʻO Borrelia Burgdorferi Nucleic Acid
inoa huahana
HWTS-OT076 Borrelia Burgdorferi Nucleic Acid Detection Kit(Fluorescence PCR)
Palapala
CE
Epidemiology
Hoʻokumu ʻia ka maʻi Lyme ma muli o ka maʻi me Borrelia burgdorferi a ua hoʻouna ʻia ma waena o nā pūʻali holoholona, ma waena o nā holoholona hoʻokipa a me nā kānaka e nā maʻi paʻakikī. Hiki i ka pathogen Borrelia burgdorferi ke hoʻoulu i ke kanaka Erythema chronicum migrans, a me nā maʻi e pili ana i nā ʻōnaehana lehulehu e like me ka puʻuwai, ke aʻalolo, a me ka hui, a me nā mea ʻē aʻe, a he ʻokoʻa nā hōʻike lapaʻau. Wahi a ka hoʻomohala ʻana o ka papa maʻi, hiki ke hoʻokaʻawale ʻia i ka maʻi maʻi kūloko mua, ka maʻi hoʻolaha waena a me ka maʻi hoʻomau mau loa, kahi mea e pōʻino nui ai i ke olakino o ka lehulehu. No laila, i ka maʻi maʻi o Borrelia burgdorferi, he mea nui ia e hoʻokumu i kahi ala maʻalahi, kikoʻī a wikiwiki hoʻi no ka maʻi etiological o Borrelia burgdorferi.
Kanal
| FAM | DNA o Borrelia burgdorferi |
| VIC/HEX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | ʻO ke koko holoʻokoʻa |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kope/mL |
| Nā mea hana pili | ABI 7500 Pūnaehana PCR manawa maoli ʻO ABI 7500 ʻO nā Pūnaehana PCR ʻOiaʻiʻo wikiwiki QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
QIAamp DNA Blood Midi Kit na Qiagen (51185).It pono e unuhiiai kulike loai ke aʻo, a ʻo ka nui elution i ʻōlelo ʻia100μL.
Koho 2.
KokoGDNA ʻōpiopioEpahu hoʻokuʻu ʻia (DP318,ʻAʻole.: JingchangMea Hoʻopaʻa Pūnaewele20210062) i hana ʻia e Tiangen Biochemical Technology (Beijing) Co., Ltd.. It pono e unuhiiai kulike loai ke aʻo, a ʻo ka nui elution i ʻōlelo ʻia100μL.
Koho 3.
ʻO Wizard® Genomic DNA Purification Kit (A1120) na Promega.It pono e unuhiiai kulike loai ke aʻo, a ʻo ka nui elution i ʻōlelo ʻia100μL.
