ʻO ka waikawa nukleika Borrelia Burgdorferi
Inoa huahana
HWTS-OT076 Borrelia Burgdorferi Nucleic Acid Detection Kit (Fluorescence PCR)
Palapala hōʻoia
CE
ʻAlapia
Hoʻokumu ʻia ka maʻi Lyme e ka maʻi Borrelia burgdorferi a laha nui ʻia ma waena o nā holoholona hoʻokipa, ma waena o nā holoholona hoʻokipa a me nā kānaka e nā ʻūpā paʻakikī. Hiki i ka pathogen Borrelia burgdorferi ke hana i ka Erythema chronicum migrans kanaka, a me nā maʻi e pili ana i nā ʻōnaehana he nui e like me ka puʻuwai, ke aʻalolo, a me nā hono, a pēlā aku, a he ʻokoʻa nā hōʻike lapaʻau. Wahi a ka ulu ʻana o ke ʻano o nā maʻi, hiki ke hoʻokaʻawale ʻia i ka maʻi kūloko mua, ka maʻi hoʻolaha waena a me ka maʻi mau loa, he mea hōʻeha nui ia i ke olakino o ka heluna kanaka. No laila, i ka hōʻoia lapaʻau o Borrelia burgdorferi, he mea nui loa ia e hoʻokumu i kahi ʻano maʻalahi, kikoʻī a wikiwiki hoʻi no ka hōʻoia etiological o Borrelia burgdorferi.
Kanal
| ʻOhana | ʻO ka DNA o Borrelia burgdorferi |
| VIC/HEX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | Hāpana koko holoʻokoʻa |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kope/mL |
| Nā Mea Hana Pili | Nā ʻōnaehana PCR Manawa Maoli ABI 7500 Nā ʻōnaehana PCR Manawa Maoli wikiwiki ABI 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P LightCycler®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Koho 1.
ʻO QIAamp DNA Blood Midi Kit na Qiagen (51185).It pono e unuhi ʻiame ke kūlike ponoi ke kuhikuhi, a ʻo ka nui o ka elution i ʻōlelo ʻia100μL.
Koho 2.
KokoGDNA ʻenomiaEpahu huki (DP318,ʻAʻole.: JingchangMoʻolelo Hāmeʻa20210062) i hana ʻia e Tiangen Biochemical Technology (Beijing) Co., Ltd.. It pono e unuhi ʻiame ke kūlike ponoi ke kuhikuhi, a ʻo ka nui o ka elution i ʻōlelo ʻia100μL.
Koho 3.
Pahu Hoʻomaʻemaʻe DNA Genomic Wizard® (A1120) na Promega.It pono e unuhi ʻiame ke kūlike ponoi ke kuhikuhi, a ʻo ka nui o ka elution i ʻōlelo ʻia100μL.
