ʻO Chlamydia Trachomatis, Neisseria Gonorrhoeae a me Trichomonas vaginalis
Inoa mea kūʻai
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae a me Trichomonas vaginalis Mea ʻImi ʻAka Nucleic (Fluorescence PCR)
Epidemiology
ʻO Chlamydia trachomatis (CT) kahi ʻano microorganism prokaryotic i paʻa paʻa i loko o nā cell eukaryotic.Hoʻokaʻawale ʻia ʻo Chlamydia trachomatis i AK serotypes e like me ke ʻano serotype.ʻO nā maʻi maʻi urogenital ka hapa nui o ka trachoma biological variant DK serotypes, a ʻike ʻia nā kāne ma ke ʻano he urethritis, hiki ke hoʻomaha ʻia me ka ʻole o ka mālama ʻana, akā ʻo ka hapa nui o lākou e lilo i ka maʻi maʻi, hoʻonui pinepine ʻia, a hiki ke hui pū ʻia me ka epididymitis, proctitis, etc.
Kanal
| FAM | ʻO Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Trichomonal vaginitis |
| VIC/HEX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | -18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | ʻO ka pulupulu wahine,Wahine vaginal swabʻAhu ʻāʻī kāne |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Nā kope/mL |
| kikoo | ʻAʻohe ʻano hoʻololi ʻē aʻe me nā pathogens maʻi STD ʻē aʻe ma waho o ka pae ʻike o ka pahu hoʻāʻo, e like me Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, etc. |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
Manaʻo ʻia ka mea hoʻihoʻi: Pipette 1mL o ka hāpana e hoʻāʻo ʻia i kahi 1.5mL o DNase/RNase-free centrifuge tube, centrifuge ma 12000rpm no 3 mau minuke, e hoʻolei i ka supernatant a mālama i ka precipitate.E hoʻohui i 200µL o ka paʻakai maʻamau i ka precipitate e hoʻomaha hou.Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hana ʻia ka unuhi e like me ke kuhikuhi no ka hoʻohana ʻana.ʻO 200µL ka nui o ka laʻana, a ʻo ka nui o ka elution i manaʻo ʻia he 80µL.
Koho 2.
Manaʻo ʻia ka mea hoʻoneʻe hoʻoneʻe: Nucleic Acid Extraction a i ʻole Cleaning Kit (YDP302).Pono e hoʻokō pono ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana.ʻO ka nui o ka elution i manaʻo ʻia he 80µL.
