Coxsackie Virus Type A16 Nucleic Acid

Hoʻohana kēia kit i ka Enzymatic Probe Isothermal Amplification (EPIA), a hoʻolālā i nā primers kikoʻī a me RNA base-containing probes (rProbe) no ka wahi i mālama nui ʻia ʻo Cox A16, a hoʻohui i ka Bst enzyme a me ka enzyme RNaseH i ka manawa like, kahi i ka hema a ʻO nā kihi ʻākau o ke kumu RNA o rProbe ua hoʻopaʻa inoa ʻia me nā pūʻulu fluorescent a me ka quencher, kēlā me kēia.E hoʻohana i ka hana DNA polymerase a me ka hana hoʻoneʻe strand o ka enzyme Bst e hoʻonui ai i ka pahuhopu e hoʻāʻo ʻia ma kahi wela mau, hiki i ka enzyme RNaseH ke hoʻokaʻawale i nā kumu RNA ma ke kaulahao hybrid target-probe, i mea e hoʻonui ai ka hui fluorescent a me ka quencher o rProbe. hoʻokaʻawale ʻia ma laila e fluorescing.Eia kekahi, hiki ke hoʻohana ʻia ka ʻāpana hema o ke koena rProbe RNA base ma ke ʻano he kumu mua e hoʻonui hou aku i kahi huahana, kahi e hōʻiliʻili hou ai i ka huahana.Hoʻonui mau ʻia ka hōʻailona fluorescent me ka hoʻokumu ʻana o ka huahana, no laila e ʻike ai i ka ʻike ʻana o ka waikawa nucleic i manaʻo ʻia.