Enterovirus 71 (EV71)
Inoa mea kūʻai
HWTS-EV003- Enterovirus 71 (EV71) Kiko ʻImi ʻAiki Nucleic (Fluorescence PCR)
Epidemiology
ʻO ka maʻi lima-wāwae-waha he maʻi lele ia e nā enteroviruses (EV).I kēia manawa, ua loaʻa nā ʻano serotypes o enteroviruses 108, i māhele ʻia i ʻehā mau pūʻulu: A, B, C a me D. I waena o lākou, enterovirus EV71 a me CoxA16 nā pathogens nui.Loaʻa ka maʻi i nā keiki ma lalo o 5 mau makahiki, a hiki ke hoʻoulu i ka herpes ma nā lima, nā wāwae, ka waha a me nā ʻāpana ʻē aʻe.E ulu ana kekahi helu liʻiliʻi o nā keiki i nā pilikia e like me ka myocarditis, ka edema pulmonary, a me ka maningoencephalitis aseptic.
Kanal
| FAM | EV71 |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | Oropharyngeal swabʻO ka wai herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 kope/mL |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR Manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
Manaʻo ʻia ka unuhi ʻana: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), a pono e mālama ʻia ka unuhi e like me ke kuhikuhi no ka hoʻohana ʻana.ʻO nā laʻana i unuhi ʻia, ʻo ia nā oropharyngeal swabs a i ʻole nā palapala wai herpes mai nā maʻi i hōʻiliʻili ma ka pūnaewele.E hoʻohui i nā swabs i hōʻiliʻili ʻia i loko o ka Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) pololei, vortex a hui maikaʻi, waiho i ka lumi wela no 5 mau minuke, lawe i waho a laila hoʻohuli e hui maikaʻi, e loaʻa ai ka RNA. o kēlā me kēia hāpana.
Koho 2.
Manaʻo ʻia ka mea hoʻihoʻi hou: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test ʻO ka mea hoʻoheheʻe Nucleic Acid Automatic (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., a pono e hoʻokō ʻia ka unuhi e like me ke kuhikuhi no ka hoʻohana ʻana.ʻO 200µL ka nui o ka laʻana, a ʻo ka nui o ka elution i manaʻo ʻia he 80µL.
Koho3.
Manaʻo ʻia ka unuhi ʻana: QIAamp Viral RNA Mini Kit (52904) na QIAGEN a i ʻole TIANamp Virus DNA/RNA Kit (YDP315-R), a pono e mālama ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana.ʻO 140μL ka nui o ka laʻana, a ʻo ka nui o ka elution i ʻōlelo ʻia he 60μL.
