ʻO ka maʻi Enterovirus 71 (EV71)
Inoa huahana
HWTS-EV003- Enterovirus 71 (EV71) Pahu ʻIke Waikawa Nukleika (Fluorescence PCR)
ʻAlapia
ʻO ka maʻi lima-wāwae-waha kahi maʻi lele i hoʻokumu ʻia e nā enteroviruses (EV). I kēia manawa, ua loaʻa he 108 mau ʻano serotypes o nā enteroviruses, i māhele ʻia i ʻehā mau pūʻulu: A, B, C a me D. I waena o lākou, ʻo ka enterovirus EV71 a me CoxA16 nā pathogens nui. Loaʻa pinepine ka maʻi i nā keiki ma lalo o 5 mau makahiki, a hiki ke hoʻoulu i ka herpes ma nā lima, nā wāwae, ka waha a me nā ʻāpana ʻē aʻe. E loaʻa i kahi helu liʻiliʻi o nā keiki nā pilikia e like me ka myocarditis, ka edema pulmonary, a me ka aseptic meningoencephalitis.
Kanal
| ʻOhana | EV71 |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 9 mahina |
| ʻAno Laʻana | ʻO ka ʻūpā oropharyngeal,Wai herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 Kope/mL |
| Nā Mea Hana Pili | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P LightCycler®480 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana ʻike PCR manawa maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Koho 1.
ʻO ka mea hoʻoheheʻe i ʻōlelo ʻia: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), a pono e hana ʻia ka hoʻoheheʻe ʻana e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO nā laʻana i unuhi ʻia, ʻo ia nā swabs oropharyngeal a i ʻole nā laʻana wai herpes mai nā maʻi i hōʻiliʻili ʻia ma kahi. E hoʻohui pololei i nā swabs i hōʻiliʻili ʻia i loko o ka Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), vortex a hui maikaʻi, e kau i ka mahana o ka lumi no 5 mau minuke, e lawe i waho a laila e hoʻohuli e hui maikaʻi, e loaʻa ai ka RNA o kēlā me kēia laʻana.
Koho 2.
Mea hoʻohuihui i ʻōlelo ʻia: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., a pono e hana ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka laʻana i unuhi ʻia he 200µL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80µL.
Koho 3.
ʻO ka mea hoʻohuihui i ʻōlelo ʻia: QIAamp Viral RNA Mini Kit (52904) e QIAGEN a i ʻole TIANamp Virus DNA/RNA Kit (YDP315-R), a pono e hana ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka laʻana i unuhi ʻia he 140μL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 60µL.
