EnteRovirus 71 (ev71)
Hua waihona
Hwts-Ev003- Enterovirus 71
Epideminey
ʻO ka maʻi lima lima-wāwae ka maʻi maʻi maʻi i hōʻehaʻia e nā mea e komo ai e nā mea e komo (ev). I kēia manawa, 108 mauʻano o nā seerotypes o nā mea kūʻai aku i loaʻa, i māheleʻia i nā pūʻuluʻehā,ʻo ko lākou mau patamgens nui. Ke hele nui nei ka maʻi ma lalo o nā keiki ma lalo o 5 mau makahiki, a hiki ke hoʻonāukiuki i nā wāwae, nā wāwae a me nā'āpana'ē aʻe. ʻO kahi helu liʻiliʻi e hoʻomohala i nā hoʻopiʻi e like me myocarditis, pulmonary edema, a me kahi mensptic megenoncephalitis.
Kauwe
| Ka ha | E.71 |
| Rox | Kaohi kūloko |
Nā poʻoaʻenehana
| Huanui | ≤-18 ℃ |
| 'Āmaʻomaʻo | 9 mau mahina |
| ʻAno kiko'ī | ʻO Oropharyngeal Swab,ʻO nā mea e kahe ana |
| Ct | Ç35 |
| CV | <5.0% |
| Lolulu | 500copies / ml |
| Nā mea pili | Noi biosysteystems 7500 mau'ōnaehana PCR Noi biosystems 7500 wikiwiki mau'ōnaehana PCR Qutudidio®5 mau'ōnaehana PCR SLAN-96P mea maoli maoli PCR Waiwai ka Lio®480 Nā'ōnaehana PCR manawa Laina jergene 9600 me nā'ōnaehanaʻo Scr Records Ma-6000 maoli-manawa nui ka manawa nui Biorad CFX96 Nā Kūlana Kūlana Lacr Biorad CFX OPus 96 Reass PCR System |
Nā kahe o ka hana
Ke koho 1.
Hoʻonohonohoʻia nā uku uku ʻO nā hiʻohiʻona i kohoʻia i nā swabs oropharyngeal a iʻole nā mea kanu wai i nā mea maʻi mai nā maʻi i hōʻiliʻiliʻia ma ka pūnaewele. E hoʻohui i nā swabs i hōʻiliʻiliʻia i loko o ka macro & micro-sex-micro-misk reving reagent (HWTS-3005-8) o kēlā me kēia hoʻohālike.
Koho 2.
Hoʻonohonohoʻia ka uku uku ʻO nā mea hoʻokūkū nucletic nucletic (HWTS-3006C, HWTS-3006b) Co., LTD., a pono e alakaʻiʻia ka mea hana e like me ke aʻo no ka hoʻohana. ʻO ka nui o ka hua'ōlelo i kohoʻia he 200μl, aʻo ka leo i'ōleloʻiaʻo Elion i'ōleloʻia he 80μl.
Koho3.
Hoʻonohonohoʻia ka uku houʻana: QIAMP VIRL RNA Mini Mi Mini Kit (52904) e Qiagus Virus a me TRDIPT. ʻO ka nui o ka hua'ōlelo i kohoʻia he 140μl, aʻo ka nui o ka leo i'ōleloʻia he 60μl.
