ʻO Enterovirus Universal, EV71 a me CoxA16
Inoa Huahana
Pahu ʻike waikawa nucleic HWTS-EV026B-Enterovirus Universal, EV71 a me CoxA16 (Fluorescence PCR)
HWTS-EV020Y/Z-Pahu ʻike ʻana i ka Enterovirus Universal, EV71 a me CoxA16 Nucleic Acid (Fluorescence PCR) i hoʻomaloʻo ʻia i ka hau
Palapala hōʻoia
CE/MDA（HWTS-EV026）
ʻAlapia
ʻO ka maʻi lima-wāwae-waha (HFMD) kahi maʻi lele maʻamau i nā keiki. Loaʻa pinepine ia i nā keiki ma lalo o 5 mau makahiki, a hiki ke hana i ka herpes ma nā lima, nā wāwae, ka waha a me nā ʻāpana ʻē aʻe, a hiki i kahi helu liʻiliʻi o nā keiki ke hana i nā pilikia e like me ka myocarditis, pulmonary edema, aseptic meningoencephalitis, a pēlā aku. ʻO kēlā me kēia keiki me nā maʻi koʻikoʻi e hōʻino koke a make koke lākou inā ʻaʻole mālama koke ʻia.
I kēia manawa, ua loaʻa he 108 mau serotypes o nā enteroviruses, i māhele ʻia i ʻehā mau pūʻulu: A, B, C a me D. He ʻokoʻa nā Enteroviruses e hoʻoulu ai i ka HFMD, akā ʻo ka enterovirus 71 (EV71) a me ka coxsackievirus A16 (CoxA16) nā mea maʻamau a ma waho aʻe o ka HFMD, hiki ke hoʻoulu i nā pilikia koʻikoʻi o ka ʻōnaehana nerve waena e like me ka meningitis, encephalitis, a me ka paralysis flaccid acute.
Kanal
| ʻOhana | ʻO ka maʻi ʻōpū |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Ka mana kūloko |
Nā Palena ʻenehana
| Ka Waihona | Wai: ≤-18 ℃ I ka pōʻeleʻeleHoʻopili ʻana: ≤30 ℃ |
| Ola papa | Wai: 9 mahinaHoʻopaʻa ʻana i ka Lyophilization: 12 mahina |
| ʻAno Laʻana | Laʻana ʻūpā ʻāʻī, wai Herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kope/mL |
| Nā Mea Hana Pili | Hiki iā ia ke hoʻokūkū i nā mea hana PCR fluorescent nui ma ka mākeke.Nā ʻōnaehana PCR Manawa Maoli ABI 7500 Nā ʻōnaehana PCR Manawa Maoli wikiwiki ABI 7500 Nā ʻōnaehana PCR Manawa Maoli SLAN-96P Nā ʻōnaehana PCR Manawa Maoli QuantStudio®5 Nā ʻōnaehana PCR Manawa Maoli LightCycler®480 Nā ʻōnaehana ʻike PCR manawa maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Hoʻonā PCR holoʻokoʻa
Mea hoʻohuihui i manaʻo ʻia: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hoʻokō ʻia ka hoʻohuihui e like me ke kuhikuhi ʻana i ka puke kuhikuhi. ʻO ka nui o ka laʻana i unuhi ʻia he 200μL, a ʻo ka nui o ka elution i manaʻo ʻia he 80μL.
ʻO ka mea hoʻoheheʻe i ʻōlelo ʻia: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Pono e hoʻokō ʻia ka hoʻoheheʻe ʻana e like me ke kuhikuhi. ʻO nā laʻana hoʻoheheʻe he mau swab oropharyngeal a i ʻole nā laʻana wai herpes o nā maʻi i hōʻiliʻili ʻia ma kahi. E hoʻohui pololei i nā swabs i hōʻiliʻili ʻia i ka Macro & Micro-Test Sample Release Reagent, vortex a kāwili maikaʻi, e kau ma ka mahana o ka lumi no 5 mau minuke, e lawe i waho a laila e hoʻohuli a kāwili maikaʻi e loaʻa ai ka RNA o kēlā me kēia laʻana.
ʻO ka mea hoʻoheheʻe i ʻōlelo ʻia: QIAamp Viral RNA Mini Kit (52904) e QIAGEN a i ʻole Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R). Pono e hoʻokō ʻia ka hoʻoheheʻe ʻana e like me ka manual kuhikuhi.
