Enterovirus Universal, EV71 a me CoxA16
Inoa Huahana
HWTS-EV026B-Enterovirus Universal, EV71 a me CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
HWTS-EV020Y/Z-Enterovirus Universal, EV71 a me CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
Palapala
CE/MDA（HWTS-EV026）
Epidemiology
ʻO ka maʻi lima wāwae-waha (HFMD) he maʻi lele maʻamau i nā keiki. Loaʻa ia i nā keiki ma lalo o 5 mau makahiki, a hiki ke hoʻoulu i ka herpes ma nā lima, nā wāwae, ka waha a me nā ʻāpana ʻē aʻe, a hiki i kahi helu liʻiliʻi o nā keiki ke hana i nā pilikia e like me ka myocarditis, pulmonary edema, aseptic meningoencephalitis, etc.
I kēia manawa, ua loaʻa nā 108 serotypes o enteroviruses, i māhele ʻia i ʻehā mau pūʻulu: A, B, C a me D. ʻO nā Enteroviruses e hoʻopiʻi ai i ka HFMD he ʻano like ʻole, akā ʻo enterovirus 71 (EV71) a me coxsackievirus A16 (CoxA16) ka mea maʻamau a me ka HFMD, hiki ke hoʻoulu i ka maʻi maʻi maʻi maʻi, a me ka maʻi maʻi maʻi maʻi.
Kanal
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Ka hooponopono kuloko |
Nā ʻāpana ʻenehana
| Waihona | Ka wai: ≤-18 ℃ I ka pōʻeleʻeleLyophilization: ≤30 ℃ |
| Kau-ola | Ka wai: 9 mahinaLyophilization: 12 mahina |
| ʻAno Hoʻohālike | ʻO ka laʻana o ka puʻu, ka wai Herpes |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 500 kope/mL |
| Nā mea hana pili | Hiki iā ia ke hoʻohālikelike i nā mea hana PCR fluorescent mainstream ma ka mākeke.ABI 7500 Pūnaehana PCR manawa maoli ʻO ABI 7500 ʻO nā Pūnaehana PCR ʻOiaʻiʻo wikiwiki SLAN-96P Pūnaehana PCR Manawa maoli QuantStudio®5 Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Huina PCR
Manaʻo ʻia ka unuhi ʻana: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hoʻokō ʻia ka unuhi e like me ka manual ʻO 200μL ka nui o ka laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 80μL.
Manaʻo ʻia ka mea hoʻoheheʻe: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Pono e hoʻokō ʻia ka unuhi e like me ka manual instruction. ʻO nā laʻana hoʻoheheʻe ʻia he oropharyngeal swabs a i ʻole nā palapala wai herpes o nā maʻi i hōʻiliʻili ʻia ma ka pūnaewele. Hoʻohui pololei i nā swabs i hōʻiliʻili ʻia i ka Macro & Micro-Test Sample Release Reagent, vortex a hui maikaʻi, waiho i ka lumi wela no 5 mau minuke, lawe i waho a laila hoʻohuli a hui maikaʻi e loaʻa ka RNA o kēlā me kēia laʻana.
Manaʻo ʻia ka mea hoʻoneʻe: QIAamp Viral RNA Mini Kit (52904) na QIAGEN a i ʻole Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R). Pono e hoʻokō ʻia ka unuhi ʻana e like me ka manual instruction.
