ʻO ka nahele encephalitis
Hua waihona
ʻO Hwts-fe006
Epideminey
ʻO ka lāʻau encephalitis (Fel),ʻikeʻiaʻo ia e like me ke poʻoʻo Tick-Broe encerolit ʻO ka lāʻau encephalitis i nā birm no ka flavivirus genus o kaʻohana flaviviridae. ʻO nā'āpana wahine he spirical me ka'āpana o ka nui o 40-50nm. ʻO ka paona molecular e pili ana i 4 × 106Da, a me ka wahine o ka wahine wahine he maikaʻi maikaʻi, hoʻokahi rna[1]. ^. Clinically,ʻo ia ka mea i hōʻikeʻia e ke poʻo kiʻekiʻe, ke poʻo,ʻo Coma, Rapid o ka'ā'ī a me nā manamana lima o ka'ā'ī a me nā wāwae ʻO ke ala maikaʻi a me ka wikiwiki o ka uluʻana o ka uluʻana o ka uluʻana o ka uluʻana o ka uluʻana o ka ulu lāʻau entngalis o ka ulu lāʻau[1,2].
Kauwe
Ka ha | ʻO ka lāʻau encephalit Spine Brus |
Rox | Kaohi kūloko |
Nā poʻoaʻenehana
Huanui | ≤-18 ℃ |
'Āmaʻomaʻo | 9 mau mahina |
ʻAno kiko'ī | ʻoihana hou |
Tt | ≤38 |
CV | ≤5.0% |
Lolulu | 500copies / ml |
Nā mea pili | Noi biosysteystems 7500 mau'ōnaehana PCR Noi biosystems 7500 wikiwiki mau'ōnaehana PCR Qutudidio®5 mau'ōnaehana PCR SLAN-96P mea maoli maoli PCR Waiwai ka Lio®480ʻO ka'ōnaehana PCR manawa Laina jergene 9600 me nā'ōnaehanaʻo Scr Records Ma-6000 maoli-manawa nui ka manawa nui Biorad CFX96 Nā Kūlana Kūlana Lacr Biorad CFX OPus 96 Reass PCR System |
Nā kahe o ka hana
Hoʻonohonohoʻia nā mea hou aku:ʻO ka nui o ka hōʻailona nuclec a iʻole ka hoʻomaʻemaʻeʻana (yd3915-r) e like me keʻano., Ltd. ʻO ka hua'ōlelo i hōʻikeʻia i hōʻikeʻiaʻo ia ka nui o ka helu he 140
Hoʻonohonohoʻia nā uku uku Hwts-3006, HWTS-3006C, HWTS-3006B). Pono e alakaʻiʻia ka extration e like me nā'ōlelo paʻa. ʻO ka hua'ōlelo i kohoʻia i kohoʻia he 200μl a me ka nui o ka leo i'ōleloʻiaʻo ka nui o ke Akua i'ōleloʻia he 80μl.