ʻO ka maʻi maʻi Encephalitis nahele
inoa huahana
HWTS-FE006 Nahele Encephalitis Virus Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
ʻO ka ululāʻau encephalitis (FE), ʻike ʻia hoʻi ʻo tick-borne encephalitis (Tick-borne encephalitis, TBE), he maʻi maʻi maʻi ʻeha o ka pūnaeho puʻupuʻu i hoʻokumu ʻia e ka ululāʻau encephalitis virus. No ka genus Flavivirus o ka ohana Flaviviridae. He spherical nā ʻāpana virus me ke anawaena o 40-50nm. ʻO ke kaumaha molekala ma kahi o 4 × 106ʻO ia, a ʻo ka genome virus he manaʻo maikaʻi, hoʻokahi-stranded RNA[1]. ʻO ka maʻi maʻi, ua hōʻike ʻia e ke kuni kiʻekiʻe, ke poʻo, ka coma, ka hoʻomaka koke ʻana o ka maningeal irritation, a me ka maʻi lolo o ka ʻāʻī a me nā lālā, a he kiʻekiʻe ka nui o ka make. ʻO ka ʻike mua a me ka wikiwiki o ka ululāʻau encephalitis virus ke kī i ka mālama ʻana i ka ululāʻau encephalitis, a ʻo ka hoʻokumu ʻana i kahi ala maʻalahi, kikoʻī a me ka wikiwiki o ka etiological etiological he mea koʻikoʻi nui ia i ka hōʻoia ʻana o ka ululāʻau encephalitis.[1,2].
Kanal
| FAM | nahele encephalitis virus nucleic acid |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | serum hou |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kope/mL |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Manaʻo ʻia ka hoʻoulu ʻana: Nucleic Acid Extraction a i ʻole Purification Kit (YDP315-R) e Tiangen Biotech(Beijing) Co., Ltd., pono e hoʻokō ʻia ka unuhi e like me nā ʻōlelo kuhikuhi. ʻO 140μL ka nui o ka laʻana i unuhi ʻia a ʻo ka nui elution i ʻōlelo ʻia he 60μL.
Manaʻo ʻia ka mea hoʻihoʻi hou: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3017-3006) HWTS-3006B). pono e hana 'ia ka unuhi e like me na kuhikuhi. ʻO 200μL ka nui o ka laʻana i unuhi ʻia a ʻo 80μL ka nui o ka elution i manaʻo ʻia.
