ʻO Chlamydia Trachomatis i hoʻomaloʻo ʻia
Inoa huahana
HWTS-UR032C/D-Pahu ʻike waikawa nucleic Chlamydia Trachomatis i hoʻomaloʻo ʻia (Enzymatic Probe Isothermal Amplification)
ʻAlapia
ʻO Chlamydia trachomatis (CT) kahi ʻano microorganism prokaryotic i parasitic wale nō i loko o nā cell eukaryotic[1]Ua māhele ʻia ʻo Chlamydia trachomatis i nā serotypes AK e like me ke ʻano serotype. ʻO nā maʻi ʻōpū Urogenital ka mea i hoʻokumu nui ʻia e nā trachoma biological variant DK serotypes, a ʻo nā kāne e hōʻike pinepine ʻia ma ke ʻano he urethritis, hiki ke hoʻomaha ʻia me ka ʻole o ka lāʻau lapaʻau, akā ʻo ka hapa nui o lākou e lilo i mau loa, hoʻonui pinepine ʻia, a hiki ke hui pū ʻia me ka epididymitis, proctitis, a pēlā aku.[2]Hiki ke hana ʻia nā wahine me ka urethritis, cervicitis, a me nā mea ʻē aʻe, a me nā pilikia koʻikoʻi o ka salpingitis.[3].
Kanal
| ʻOhana | ʻO Chlamydia trachomatis (CT) |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤30 ℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | ʻO ka swab cervical wahine ʻO ka swab urethral kāne Mimi kāne |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | 400 Kope/mL |
| Kūlana kikoʻī | ʻaʻohe keʻa-reactivity ma waena o kēia pahu a me nā pathogens maʻi ʻāpana genitourinary ʻē aʻe e like me ke kiʻekiʻe-pilikia Kanaka papillomavirus type 16, Kanaka papillomavirus type 18, Herpes simplex virus type Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus epidermidis, Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Kanaka immunodeficiency virus, Lactobacillus casei a me ke kanaka genomic DNA, etc. |
| Nā Mea Hana Pili | ʻŌnaehana PCR Manawa Maoli Biosystems 7500 i hoʻopili ʻia Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 a me ka ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 ʻŌnaehana ʻIke Isothermal Fluorescence manawa maoli maʻalahi Amp（HWTS-1600）. |
Kahe Hana
Koho 1.
Mea Hoʻokuʻu Laʻana Macro & Micro-Test (HWTS-3005-8). Pono e hoʻokō ʻia ka unuhi ʻana e like me ka IFU. E hoʻohui i ka DNA laʻana i unuhi ʻia e ka mea hoʻokuʻu laʻana i loko o ka buffer reaction a hoʻāʻo pololei ma ka mea hana, a i ʻole e mālama ʻia nā laʻana i unuhi ʻia ma 2-8 ℃ no ka ʻoi aku ma mua o 24 mau hola.
Koho 2.
Pahu DNA/RNA Laulā no ka Hoʻāʻo Macro a me Micro (HWTS-301)7-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) a me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Pono e hoʻokō ʻia ka unuhi ʻana e like me ka IFU, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80μL. Hoʻomehana ʻia ka DNA laʻana i unuhi ʻia e ke ʻano bead magnetic ma 95°C no 3 mau minuke a laila holoi koke ʻia i ka hau no 2 mau minuke. E hoʻohui i ka DNA laʻana i hana ʻia i loko o ka buffer reaction a hoʻāʻo ma ka mea hana a i ʻole e mālama ʻia nā laʻana i hana ʻia ma lalo o -18°C no ka ʻoi aku ma mua o 4 mau mahina. ʻAʻole pono e ʻoi aku ka nui o ka hoʻopaʻa ʻana a me ka hoʻoheheʻe ʻana ma mua o 4 mau pōʻaiapuni.
