Chlamydia Trachomatis maloʻo paʻa
inoa huahana
HWTS-UR032C/D-Chlamydia Trachomatis Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)
Epidemiology
ʻO Chlamydia trachomatis (CT) kahi ʻano microorganism prokaryotic i paʻa paʻa i nā cell eukaryotic.[1]. Hoʻokaʻawale ʻia ʻo Chlamydia trachomatis i AK serotypes e like me ke ʻano serotype. ʻO nā maʻi maʻi urogenital ka mea maʻamau e ka trachoma biological variant DK serotypes, a ʻike ʻia nā kāne ma ke ʻano he urethritis, hiki ke hoʻomaha ʻia me ka ʻole o ka mālama ʻana, akā ʻo ka hapa nui o lākou e lilo i mea maʻi, hoʻonui pinepine ʻia, a hiki ke hui pū ʻia me ka epididymitis, proctitis, etc.[2]. Hiki i nā wahine ke loaʻa i ka urethritis, cervicitis, etc., a me nā pilikia koʻikoʻi o ka salpingitis[3].
Kanal
| FAM | Chlamydia trachomatis (CT) |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤30 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | ʻAi ʻāwīwī wahine ʻAhu ʻāʻī kāne Mimi kāne |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | 400 kope/mL |
| kikoo | ʻaʻohe ʻano hana kea ma waena o kēia kit a me nā mea maʻi maʻi maʻi maʻi genitourinary ʻē aʻe e like me ke ʻano kiʻekiʻe o Human papillomavirus type 16, Human papillomavirus type 18, Herpes simplex virus type Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus, Epidermischelis, epidermis. Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei a me DNA genomic kanaka, etc. |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR manawa maoli(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli ʻO BioRad CFX96 Pūnaehana PCR manawa maoli a me BioRad CFX Opus 96 Pūnaehana PCR manawa maoli. Easy Amp Real-time Fluorescence Isothermal Detection System（HWTS-1600）. |
Holo Hana
Koho 1.
Macro & Micro-Test Sample Reagent Reagent (HWTS-3005-8). Pono e hoʻokō ʻia ka unuhi ʻana e like me ka IFU. E hoʻohui i ka DNA hāpana i unuhi ʻia e ka hāpana hoʻokuʻu reagent i loko o ka pahu hoʻonā a hoʻāʻo pololei i ka mea kani, a i ʻole e mālama ʻia nā laʻana i unuhi ʻia ma 2-8 ℃ no ka 24 mau hola.
Koho 2.
ʻO ka Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Pono e hoʻokō ʻia ka unuhi ʻana e like me ka IFU, a ʻo ka nui elution i ʻōlelo ʻia he 80μL. Hoʻomaʻamaʻa ʻia ka DNA hāpana i unuhi ʻia e ke ʻano kuʻi magnetic ma 95°C no 3 mau minuke a laila ʻauʻau koke i ka hau no 2 mau minuke. E hoʻohui i ka DNA hāpana i hana ʻia i loko o ka pahu hoʻoheheʻe a hoʻāʻo i ka mea hana a i ʻole nā mea hoʻohālike i hana ʻia e mālama ʻia ma lalo o -18°C no ka ʻoi aku o 4 mahina. ʻAʻole pono e ʻoi aku ka nui o ka hoʻoheheʻe ʻana a me ka hoʻoheheʻe ʻana ma mua o 4 mau manawa.
