ʻO ka maʻi nucleic Hantaan
Inoa huahana
Pahu ʻIke Waikawa Nukleika HWTS-FE005 Hantaan Virus (Fluorescence PCR)
ʻAlapia
He ʻano maʻi RNA i hoʻopuni ʻia, ʻāpana ʻia, a kaula maikaʻi ʻole ʻo Hantavirus. Ua māhele ʻia ʻo Hantavirus i ʻelua ʻano: hoʻokahi ke kumu o ka Hantavirus pulmonary syndrome (HPS), a ʻo kekahi ke kumu o ka Hantavirus hemorrhagic fever me ka renal syndrome (HFRS). ʻO ka mea mua ka mea nui ma ʻEulopa a me ʻAmelika Hui Pū ʻIa, a ʻo ka mea hope he hemorrhagic fever me ka renal syndrome i hoʻokumu ʻia e ka maʻi Hantaan, kahi mea maʻamau ma Kina. ʻO nā hōʻailona o ka hantavirus hantaan ʻano nui e hōʻike ʻia ma ke ʻano he hemorrhagic fever me ka renal syndrome, kahi i hōʻike ʻia e ke kuni kiʻekiʻe, hypotension, kahe koko, oliguria a i ʻole polyuria a me nā hemahema hana renal ʻē aʻe. He pathogenic ia i nā kānaka a pono e uku nui ʻia.
Kanal
| ʻOhana | ʻano hantavirus hantaan |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 9 mahina |
| ʻAno Laʻana | serum hou |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Kope/μL |
| Nā Mea Hana Pili | ʻŌnaehana PCR Manawa Maoli Biosystems 7500 i hoʻopili ʻia Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P LightCycler®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Nā mea hoʻohuihui i ʻōlelo ʻia: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hana ʻia ka unuhi ʻana e like me ka IFU. ʻO ka nui o ka laʻana unuhi he 200μL. ʻO ka nui o ka elution i ʻōlelo ʻia he 80μL.
Nā mea hoʻohuihui i ʻōlelo ʻia: Nucleic Acid Extraction a i ʻole Purification Kit (YDP315-R). Pono e hana ʻia ka unuhi ʻana e like me ka IFU. ʻO ka nui o ka laʻana unuhi he 140μL. ʻO ka nui o ka elution i ʻōlelo ʻia he 60μL.
