Hepatitis A Virus
Inoa mea kūʻai
HWTS-HP005 Hepatitis A Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi Hepatitis A (HAV) ke kumu nui o ka maʻi maʻi maʻi maʻi maʻi.ʻO ka maʻi maʻi he maʻi maʻi RNA kaula hoʻokahi-manaʻo maikaʻi a no ka Hepadnavirus genus o ka ʻohana Picornaviridae.ʻO ka maʻi Hepatitis A, i lawe nui ʻia e ka fecal-oral route, kūpaʻa i ka wela, nā waikawa, a me ka hapa nui o nā mea hoʻoheheʻe kūlohelohe, hiki ke ola no ka manawa lōʻihi i loko o ka iʻa, ka wai, ka lepo, a i ʻole nā ʻili kai [1-3].Hoʻouna ʻia ia ma o ka loaʻa ʻana o ka meaʻai a i ʻole ka wai i hoʻohaumia ʻia, a i ʻole ka lawe pololei ʻia mai ke kanaka a i ke kanaka.ʻO nā meaʻai e pili ana i ka HAV, ʻo ia nō nā ʻoʻo a me nā pā, nā strawberries, nā raspberries, blueberries, nā lā, nā lau lau ʻōmaʻomaʻo, a me nā tōmato semi-dryed [4‒6].
Kanal
| FAM | HAV nucleic acid |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | Ka wai: 9 mahina, Lyophilized: 12 mahina |
| ʻAno Hoʻohālike | Serum/stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 2 Kope/μL |
| kikoo | E hoʻohana i nā kits e hoʻāʻo ai i nā maʻi maʻi hepatitis ʻē aʻe e like me ka hepatitis B, C, D, E, enterovirus 71, virus coxsackie, virus Epstein-Barr, norovirus, HIV a me ka genome kanaka. ʻAʻohe cross-reactivity. |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR manawa maoli(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Nā laʻana serum
Koho 1.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Pono e unuhiʻia e like me nā kuhikuhi.ʻO ka nui o ka elution i manaʻo ʻia he 80µL.
Koho 2.
ʻO TIANamp Virus DNA/RNA Kit (YDP315-R) i hana ʻia e Tiangen Biotech (Beijing) Co., Ltd. Pono e unuhi ʻia e like me nā kuhikuhi.ʻO 140μL ka nui o ka laʻana i unuhi ʻia.ʻO ka nui o ka elution i manaʻo ʻia he 60µL.
2.Nā Laʻana Kūʻai
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Pono e unuhiʻia e like me nā kuhikuhi.ʻO ka nui o ka elution i manaʻo ʻia he 80µL.
