Ka maʻi Hepatitis A
Inoa huahana
Pahu ʻIke Waikawa Nukleika no ka Maʻi Hepatitis A HWTS-HP005 (Fluorescence PCR)
ʻAlapia
ʻO ka maʻi Hepatitis A (HAV) ke kumu nui o ka maʻi hepatitis viral koʻikoʻi. He maʻi RNA kaula hoʻokahi maikaʻi ka maʻi a no ka ʻano Hepadnavirus o ka ʻohana Picornaviridae. ʻO ka maʻi Hepatitis A, i hoʻolaha nui ʻia ma ke ala fecal-oral, kūpaʻa i ka wela, nā waikawa, a me ka hapa nui o nā mea hoʻoheheʻe organik, hiki ke ola no ka manawa lōʻihi i loko o nā iʻa paʻi, ka wai, ka lepo, a i ʻole nā mea hoʻoheheʻe kai [1-3]. Hoʻolaha ʻia ma ka loaʻa ʻana o ka meaʻai a i ʻole ka wai i haumia, a i ʻole i hoʻolaha pololei ʻia mai ke kanaka a i ke kanaka. ʻO nā meaʻai e pili ana me HAV e komo pū me nā oysters a me nā clams, nā strawberries, nā raspberries, nā blueberries, nā lā, nā lau ʻai ʻōmaʻomaʻo, a me nā tōmato maloʻo hapa [4‒6].
Kanal
| ʻOhana | ʻAkika nukleika HAV |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | Wai: 9 mahina, Lyophilized: 12 mahina |
| ʻAno Laʻana | Serum/noho |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 2 Kope/μL |
| Kūlana kikoʻī | E hoʻohana i nā kime e hoʻāʻo i nā maʻi hepatitis ʻē aʻe e like me ka hepatitis B, C, D, E, enterovirus 71, coxsackie virus, Epstein-Barr virus, norovirus, HIV a me ka genome kanaka. ʻAʻohe cross-reactivity. |
| Nā Mea Hana Pili | ʻŌnaehana PCR Manawa Maoli Biosystems 7500 i hoʻopili ʻia Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.) ʻŌnaehana PCR Manawa Maoli LightCycler®480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus (FQD-96A, ʻenehana Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Nā hāpana serum
Koho 1.
ʻO ka Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) a me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Pono e unuhi ʻia e like me nā kuhikuhi. ʻO ka nui o ka elution i ʻōlelo ʻia he 80µL.
Koho 2.
ʻO ka TIANamp Virus DNA/RNA Kit (YDP315-R) i hana ʻia e Tiangen Biotech (Beijing) Co., Ltd. Pono e unuhi ʻia e like me nā kuhikuhi. ʻO ka nui o ka laʻana i unuhi ʻia he 140μL. ʻO ka nui o ka elution i ʻōlelo ʻia he 60µL.
2.Nā Laʻana Noho
ʻO ka Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) a me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Pono e unuhi ʻia e like me nā kuhikuhi. ʻO ka nui o ka elution i ʻōlelo ʻia he 80µL.
