Hepatitis B Virus Genotyping
inoa huahana
HWTS-HP002-Hepatitis B Virus Genotyping Detection Kit (Fluorescent PCR)
Epidemiology
Epidemiology
Kanal
| KanalInoa | Hoʻopaʻa pānaʻi 1 | Paʻa Paʻa 2 |
| FAM | HBV-C | HBV-D |
| VIC/HEX | HBV-B | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ I ka pōʻeleʻele |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | Serum, Plasma |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 1×102IU/mL |
| kikoo | ʻAʻohe ʻano hoʻopaʻapaʻa me ka maʻi hepatitis C, cytomegalovirus kanaka, Epstein-Barr virus, human immunodeficiency virus, hepatitis A virus, syphilis, herpes virus, influenza A virus, propionibacterium acnes (PA), etc. |
| Nā mea hana pili | Hiki iā ia ke hoʻohālikelike i nā mea hana PCR fluorescent mainstream ma ka mākeke. ABI 7500 Pūnaehana PCR manawa maoli ʻO ABI 7500 ʻO nā Pūnaehana PCR ʻOiaʻiʻo wikiwiki SLAN-96P Pūnaehana PCR Manawa maoli QuantStudio®5 Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Manaʻo ʻia extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.200μL, a ʻo ka nui elution i ʻōlelo ʻia80μL.
Manaʻo ʻia ka mea hoʻohui: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315). Pono e hoʻokō ʻia ka unuhi ʻana e like me nā kuhikuhi. ʻO 200µL ka nui o ka laʻana, a ʻo ka nui o ka elution i ʻōlelo ʻia he 100µL.
