Hepatitis E Virus
inoa huahana
HWTS-HP006 Hepatitis E Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi Hepatitis E (HEV) he maʻi RNA e hoʻopilikia i ke olakino honua. Loaʻa iā ia kahi ākea ākea a loaʻa iā ia ka waiwai o ka hele ʻana i nā pale interspecies. ʻO ia kekahi o nā pathogens zoonotic koʻikoʻi a hoʻopilikia nui i ke kanaka a me nā holoholona. Hoʻouna ʻia ʻo HEV ma o ka fecal-oral transmission, a hiki ke hoʻouna ʻia ma ke kū pololei ma o nā embryos a i ʻole ke koko. Ma waena o lākou, ma ke ala hoʻouna fecal-waha, hoʻolaha nui ka wai a me ka meaʻai i hoʻohaumia ʻia e HEV, a he kiʻekiʻe ka pilikia o ka maʻi HEV i nā kānaka a me nā holoholona [1-2].
Kanal
| FAM | HEV nucleic acid |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | Puʻu ʻāʻī |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kope/μL |
| kikoo | ʻO ka maʻi Hepatitis E (HEV) he maʻi RNA e hoʻopilikia i ke olakino honua. Loaʻa iā ia kahi ākea ākea a loaʻa iā ia ka waiwai o ka hele ʻana i nā pale interspecies. ʻO ia kekahi o nā pathogens zoonotic koʻikoʻi a hoʻopilikia nui i ke kanaka a me nā holoholona. Hoʻouna ʻia ʻo HEV ma o ka fecal-oral transmission, a hiki ke hoʻouna ʻia ma ke kū pololei ma o nā embryos a i ʻole ke koko. Ma waena o lākou, ma ke ala hoʻouna fecal-waha, hoʻolaha nui ka wai a me ka meaʻai i hoʻohaumia ʻia e HEV, a he kiʻekiʻe ka pilikia o ka maʻi HEV i nā kānaka a me nā holoholona [1-2]. |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR System QuantStudio®5 Pūnaehana PCR Manawa maoli SLAN-96P Pūnaehana PCR manawa maoli(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻokele Thermal Cycler Manawa maoli (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006C). Pono e unuhiʻia e like me nā kuhikuhi. ʻO ka nui o ka elution i manaʻo ʻia he 80µL.
Koho 2
ʻO TIANamp Virus DNA/RNA Kit (YDP315-R) i hana ʻia e Tiangen Biotech (Beijing) Co., Ltd. Pono e unuhi ʻia e like me nā kuhikuhi. ʻO 140μL ka nui o ka laʻana i unuhi ʻia. ʻO ka nui o ka elution i manaʻo ʻia he 60µL.v
