Ka maʻi Hepatitis E
Inoa huahana
Pahu ʻIke Waikawa Nukleika no ka Maʻi Hepatitis E HWTS-HP006 (Fluorescence PCR)
ʻAlapia
ʻO ka maʻi Hepatitis E (HEV) he maʻi RNA e hoʻoulu ai i nā pilikia olakino honua. Loaʻa iā ia kahi laulā ākea a loaʻa iā ia ka waiwai o ke keʻa ʻana i nā pale interspecies. ʻO ia kekahi o nā pathogens zoonotic koʻikoʻi a hōʻeha nui i ke kanaka a me nā holoholona. Hoʻolaha nui ʻia ka HEV ma o ka hoʻoili ʻana o ka fecal-oral, a hiki ke hoʻoili ʻia ma ke ʻano kū pololei ma o nā embryos a i ʻole ke koko. I waena o lākou, ma ke ala hoʻoili fecal-oral, ua pālahalaha nui ka wai a me ka meaʻai i haumia ʻia e HEV, a kiʻekiʻe ka pilikia o ka maʻi HEV i nā kānaka a me nā holoholona [1-2].
Kanal
| ʻOhana | ʻAkika nukleika HEV |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | ʻUhi ʻāʻī |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kope/μL |
| Kūlana kikoʻī | ʻO ka maʻi Hepatitis E (HEV) he maʻi RNA e hoʻoulu ai i nā pilikia olakino honua. Loaʻa iā ia kahi laulā ākea a loaʻa iā ia ka waiwai o ke keʻa ʻana i nā pale interspecies. ʻO ia kekahi o nā pathogens zoonotic koʻikoʻi a hōʻeha nui i ke kanaka a me nā holoholona. Hoʻolaha nui ʻia ka HEV ma o ka hoʻoili ʻana o ka fecal-oral, a hiki ke hoʻoili ʻia ma ke ʻano kū pololei ma o nā embryos a i ʻole ke koko. I waena o lākou, ma ke ala hoʻoili fecal-oral, ua pālahalaha nui ka wai a me ka meaʻai i haumia ʻia e HEV, a kiʻekiʻe ka pilikia o ka maʻi HEV i nā kānaka a me nā holoholona [1-2]. |
| Nā Mea Hana Pili | ʻŌnaehana PCR Manawa Maoli Biosystems 7500 i hoʻopili ʻia ʻŌnaehana PCR Manawa Maoli Wikiwiki Biosystems 7500 i hoʻopili ʻia Nā ʻōnaehana PCR Manawa Maoli QuantStudio®5 Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.) ʻŌnaehana PCR Manawa Maoli LightCycler®480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻopololei Wela Manawa Maoli (Suzhou Molarray Co., Ltd.) ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Koho 1
ʻO ka Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) a me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Pono e unuhi ʻia e like me nā kuhikuhi. ʻO ka nui o ka elution i ʻōlelo ʻia he 80µL.
Koho 2
ʻO ka TIANamp Virus DNA/RNA Kit (YDP315-R) i hana ʻia e Tiangen Biotech (Beijing) Co., Ltd. Pono e unuhi pono ʻia e like me nā kuhikuhi. ʻO ka nui o ka laʻana i unuhi ʻia he 140μL. ʻO ka nui o ka elution i ʻōlelo ʻia he 60µL.v
