Ka Hoʻololi ʻana o ka Gene Fusion PML-RARA kanaka
Inoa huahana
HWTS-TM017APahu ʻIke Hoʻololi Gene PML-RARA Fusion Kanaka (Fluorescence PCR)
ʻAlapia
ʻO ka leukemia promyelocytic acute (APL) kahi ʻano kūikawā o ka leukemia myeloid acute (AML). Ma kahi o 95% o nā maʻi APL e hele pū ʻia me kahi loli cytogenetic kūikawā, ʻo ia hoʻi ʻo t(15;17)(q22;q21), ka mea e hoʻohui ai i ka gene PML ma ka chromosome 15 a me ka retinoic acid receptor α gene (RARA) ma ka chromosome 17 e hana i ka gene fusion PML-RARA. Ma muli o nā wahi haki like ʻole o ka gene PML, hiki ke hoʻokaʻawale ʻia ka gene fusion PML-RARA i ke ʻano lōʻihi (ʻano L), ke ʻano pōkole (ʻano S) a me ke ʻano ʻokoʻa (ʻano V), e helu ana ma kahi o 55%, 40% a me 5% pakahi.
Kanal
| ʻOhana | ʻO ke kine hoʻohuihui PML-RARA |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18 ℃ I ka pōʻeleʻele |
| Ola papa | 9 mahina |
| ʻAno Laʻana | iwi ʻiʻo |
| CV | <5.0% |
| LoD | 1000 Kope/mL. |
| Kūlana kikoʻī | ʻAʻohe cross-reactivity me nā genes fusion ʻē aʻe ʻo BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, a me TEL-AML1 fusion genes |
| Nā Mea Hana Pili | ʻŌnaehana PCR Manawa Maoli Biosystems 7500 i hoʻopili ʻia Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻopololei Wela Manawa Maoli (Suzhou Molarray Co., Ltd.) ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Mea hoʻohuihui i manaʻo ʻia: RNAprep Pure Blood Total RNA Extraction Kit (DP433). Pono e hana ʻia ka unuhi ʻana e like me ka IFU.
