ʻO ke kanaka PML-RARA Fusion Gene Mutation
inoa huahana
HWTS-TM017APML-RARA Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi maʻi maʻi promyelocytic acute (APL) kahi ʻano kūikawā o ka maʻi leukemia myeloid acute (AML). Ma kahi o 95% o nā poʻe maʻi APL e hele pū ʻia me kahi hoʻololi cytogenetic kūikawā, ʻo ia hoʻi ʻo t(15;17)(q22;q21), ka mea e hana ai i ka gene PML ma ka chromosome 15 a me ka retinoic acid receptor α gene (RARA) ma ka chromosome 17 i hoʻohui ʻia e hana i ka PML-RARA fusion gene. Ma muli o nā haʻihaʻi like ʻole o ka gene PML, hiki ke hoʻokaʻawale ʻia ka gene fusion PML-RARA i ke ʻano lōʻihi (ʻano L), ʻano pōkole (ʻano S) a me nā ʻano ʻano like ʻole (ʻano V), helu ʻia ma kahi o 55%, 40% a me 5% pakahi.
Kanal
| FAM | PML-RARA fusion gene |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ I ka pōʻeleʻele |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | iwi iwi |
| CV | <5.0％ |
| LoD | 1000 kope/mL. |
| kikoo | ʻAʻohe ʻano hoʻololi keʻa me nā genes hui ʻē aʻe BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, a me TEL-AML1 fusion genes |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Manaʻo ʻia ka mea hoʻoheheʻe: RNAprep Pure Blood Total RNA Extraction Kit (DP433). Pono e hana ʻia ka ʻili e like me ka IFU.
