Pahu ʻike waikawa nucleic virus Ebola Bundybugyo (Fluorescence PCR)
Inoa huahana
Pahu ʻike waikawa nucleic virus Ebola Bundybugyo (Fluorescence PCR)
ʻAlapia
ʻO Filoviridae. ʻO ia ke kolu o ka maʻi Ebola hiki ke hoʻoulu i nā maʻi ahulau nui kanaka,ma hope o Zaire ebolavirus (EBOV) a me Sudan ebolavirus (SUDV). He hoʻokahi wale nō ʻo BDBVhe maʻi RNA kaula maikaʻi ʻole me ka genome o kahi 19 kb, e hoʻopili ana iā 7kūkulu ʻananā protein a me 2 mau protein ʻaʻole i kūkulu ʻia. ʻO ka manawa hoʻoulu o ka maʻi BDBV he 2-21nā lā (ʻawelika 6.3 mau lā). Ua like loa kona mau hōʻailona me nā maʻi Ebola ʻē aʻea ʻaʻole hiki ke hoʻokaʻawale ʻia e nā hōʻike lapaʻau wale nō. I ʻAukake 2007, heua kū mai ka maʻi ahulau viral i wehewehe ʻole ʻia ma ka ʻĀpana ʻo Bundibugyo okomohana o Uganda. Ua ʻike ʻia ʻo ia he maʻi Ebola hou a ua kapa ʻia ʻo Bundibugyoebolavirus (BDBV). He 93 mau hihia i manaʻo ʻia, 56 mau hihia i hōʻoia ʻia, a me 37 mau make(ka nui o ka make ʻana o ka hihia he 25%). I ka makahiki 2012, ua loaʻa hou kahi maʻi ahulau ma ka Panalāʻau ʻo Orientale oLepupalika Demokarata o Kongo, me 38 mau hihia i hōʻoia ʻia a me 13 mau make (make o ka hihiahelu 34%). I Mei 2026, ua hoʻolaha ka WHO i ka maʻi ahulau BDBV o kēia manawa maHe pilikia olakino lehulehu ka Repubalika Demokarata o Kongo a me UgandaHopohopo Honua (PHEIC). Ma ka lā 20 o Mei, aia he 246 mau hihia i kānalua ʻia a me 80nā make.
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 12nā mahina |
| ʻAno Laʻana | koko holoʻokoʻa, serum/plasma,oʻūpā ral |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 Kope/mL |
| Nā Mea Hana Pili | ʻAno I:ʻŌnaehana PCR Manawa Maoli wikiwiki ABI 7500/7500, ʻŌnaehana PCR Manawa Maoli QuantStudio®5, ʻŌnaehana PCR Manawa Maoli QuantStudio®7, ʻŌnaehana Nānā PCR Lapaʻau piha ʻakomi ʻo Hongshi SLAN-96P/S, ʻŌnaehana PCR Manawa Maoli LightCycler®480, ʻŌnaehana PCR Manawa Maoli ʻo Hangzhou Bioer FQD-96A, ʻŌnaehana PCR Manawa Maoli ʻo Yarui MA-6000, ʻŌnaehana PCR Manawa Maoli BioRad CFX96, ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96. ʻAno II: ʻŌnaehana ʻIke Molekala ʻakomi ʻo Eudemon AIO800 (HWTS-AIO800)
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Kahe hana:
ʻAno I:
ʻAno II：
Nā Reagent i hoʻomākaukau ponoʻī ʻiaNā mea hoʻohuihui saline physiological, nā mea hoʻoheheʻe waikawa nucleic
Nā mea i hoʻomākaukau ponoʻī ʻiaNā wēlau pipette ʻole DNase/RNase, nā mīkina lima hoʻolei, 1.5 mL
Nā ʻōmole centrifuge ʻole DNase/RNase, centrifuge
