Influenza A/B
Inoa mea kūʻai
HWTS-RT003A Influenza A/B Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi Influenza A he maʻi maʻi ʻeha o ka hanu, me nā ʻano ʻano like ʻole e like me H1N1 a me H3N2, hiki ke hoʻololi a puka i ka honua.ʻO ka hoʻololi antigenic e pili ana i ka hoʻololi ʻana o ka maʻi maʻi influenza A, ka hopena i ka puka ʻana mai o kahi subtype hou.Ua māhele ʻia nā maʻi maʻi Influenza B i ʻelua mau laina nui, ʻo Yamagata a me Victoria.He antigenic drift wale nō nā maʻi maʻi Influenza B, a pale lākou i ka nānā ʻana a me ka hoʻomaʻemaʻe ʻana o ka ʻōnaehana pale kanaka ma o kā lākou hoʻololi ʻana.Eia nō naʻe, ʻoi aku ka lohi o ka ulu ʻana o ka maʻi maʻi influenza B ma mua o ka maʻi maʻi influenza A, a hiki i ka maʻi maʻi influenza B ke hoʻoulu i ka maʻi hanu kanaka a alakaʻi i nā maʻi maʻi.
Kanal
| FAM | IFV A |
| ROX | Hoomalu Kuloko |
| VIC/HEX | IFV B |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | oropharyngeal swab |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | IFV A:500 kope/mL，IFV B:500 kope/mL |
| kikoo | 1. Cross-reactivity: ʻaʻohe hana kea ma waena o kēia pahu a me ka adenovirus type 3, 7, ka coronavirus kanaka SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, a me HCoV-NL63, cytomegalovirus, enterovirus, ma'i ma'i parainfluenza, ma'i ma'i 'a'ai, ma'i metapneumovirus kanaka, ma'i mumps, ma'i ma'i respiratory syncytial type B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacillus, moraxella catarrhalis, myoplasma catarrhalis, myoplasma catarrhalis, myoplasma catarrhalis. , neisseria gonorrhoeae, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, streptococcus salivarius a me DNA genomic kanaka. 2. Ho'āʻo Interference: Ua koho ʻia nā mea hoʻopili e like me mucin (60mg/mL), koko kanaka, oxymetazoline (2mg/mL), sulfur (10%), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide ( 20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL). ), azithromycin (1mg / L), cephalosporin (40μg / mL), mupirocin (20mg / mL), tobramycin (0.6mg / mL), oseltamivir phosphate (60ng / mL), ribavirin (10mg / L), a me nā hopena e hōʻike ana. ʻaʻohe hopena keʻakeʻa i ka ʻike ʻana o ke kit i nā mea hoʻopilikia me nā ʻike ma luna. |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR manawa maoli(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli (Suzhou Molarray Co., Ltd.) ʻO BioRad CFX96 Pūnaehana PCR manawa maoli a me BioRad CFX Opus 96 Pūnaehana PCR manawa maoli. |
Holo Hana
Koho 1.
Manaʻo ʻia nā reagents unuhi: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test ʻO ka mea hoʻoheheʻe Nucleic Acid Automatic (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hana ʻia ka unuhi e like me ka IFU.ʻO ka nui o ka laʻana unuhi200μL.ʻO ka nui o ka elution i manaʻo ʻia he 80μL.
Koho 2.
Manaʻo ʻia nā mea hoʻoulu hou: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Pono e hana ʻia ka ʻili e like me ka IFU.
Koho 3.
Manaʻo ʻia nā mea hoʻoulu hou: Nucleic Acid Extraction or Purification Kit (YDP315-R).Pono e hana ʻia ka ʻili e like me ka IFU.ʻO 140μL ka nui o ka laʻana.ʻO ka nui o ka elution i manaʻo ʻia he 60μL.
