ʻO Klebsiella Pneumoniae, Acinetobacter Baumannii a me Pseudomonas Aeruginosa a me nā Genes Resistance Drug (KPC, NDM, OXA48 a me IMP) Multiplex

Wehewehe Pōkole:

Hoʻohana ʻia kēia pahu hana no ka ʻike ʻana i ka Klebsiella pneumoniae (KPN), Acinetobacter baumannii (Aba), Pseudomonas aeruginosa (PA) a me ʻehā mau genes kūʻē carbapenem (e komo pū ana me KPC, NDM, OXA48 a me IMP) i loko o nā laʻana sputum kanaka, e hāʻawi i ke kumu o ke alakaʻi ʻana i ka hōʻailona lapaʻau, ka mālama ʻana a me ka lāʻau lapaʻau no nā poʻe maʻi me ka maʻi bacteria i manaʻo ʻia.

E leka uila mai iā mākou

Nā kikoʻī huahana

Nā Lepili Huahana

Inoa huahana

ʻO HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii a me Pseudomonas Aeruginosa a me nā Genes Resistance Drug (KPC, NDM, OXA48 a me IMP) Multiplex Detection Kit (Fluorescence PCR)

Palapala hōʻoia

ʻAlapia

ʻO Klebsiella pneumoniae kahi pathogen opportunistic maʻamau a ʻo ia kekahi o nā bacteria pathogenic koʻikoʻi e hoʻoulu ai i nā maʻi nosocomial. Ke emi ka pale ʻana o ke kino, komo nā bacteria i loko o nā māmā mai ke ala hanu, e hoʻoulu ai i ka maʻi ma nā ʻāpana he nui o ke kino, a ʻo ka hoʻohana mua ʻana i nā antibiotics ke kī i ka ho'ōla.^[1].

ʻO ke kahua maʻamau o ka maʻi Acinetobacter baumannii ʻo ia nā māmā, he pathogen koʻikoʻi ia no ka maʻi pneumonia i loaʻa i ka haukapila (HAP), ʻoiai ʻo ka Ventilator associated pneumonia (VAP). Hoʻopili pinepine ʻia me nā maʻi bacteria a me nā fungal ʻē aʻe, me nā ʻano o ka nui o ka maʻi a me ka nui o ka make.

ʻO Pseudomonas aeruginosa ka bacilli gram-negative non-fermentative maʻamau i ka hana lapaʻau, a he pathogen opportunistic koʻikoʻi no ka maʻi i loaʻa i ka haukapila, me nā ʻano o ka colonization maʻalahi, ka loli maʻalahi a me ke kū'ē ʻana i nā lāʻau he nui.

Kanal

Inoa	PCR-Mix 1	PCR-Mix 2
Kanal FAM	ʻAba	IMP
Kahawai VIC/HEX	Mana Kūloko	KPC
Kanal CY5	PA	NDM
Kanal ROX	KPN	OXA48

Nā Palena ʻenehana

Ka Waihona	≤-18℃
Ola papa	12 mahina
ʻAno Laʻana	ʻŌhū
Ct	≤36
CV	≤10.0%
LoD	1000 CFU/mL
Kūlana kikoʻī	a) Hōʻike ka hoʻāʻo cross-reactivity ʻaʻohe cross reactivity o kēia pahu me nā pathogens hanu ʻē aʻe, e like me Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus a me nā laʻana sputum me nā pahuhopu ʻole, a pēlā aku. b) Ka hiki ke kū'ē i ke komo ʻana: E koho i ka mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, a me roxithromycin, a pēlā aku no ka hoʻāʻo komo ʻana, a hōʻike nā hopena ʻaʻole i komo nā mea komo i ʻōlelo ʻia ma luna nei i ka ʻike ʻana i nā genes kū'ē Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa a me carbapenem KPC, NDM, OXA48 a me IMP.
Nā Mea Hana Pili	Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio^®5 mau ʻōnaehana PCR Manawa Maoli LightCycler^®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus (FQD-96A, ʻenehana Bioer) MA-6000 Mea Hoʻopololei Wela Manawa Maoli (Suzhou Molarray Co., Ltd.) ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96