Klebsiella Pneumoniae, Acinetobacter Baumannii a me Pseudomonas Aeruginosa a me Drug Resistance Genes (KPC, NDM, OXA48 a me IMP) Multiplex

ʻO ka wehewehe pōkole:

Hoʻohana ʻia kēia kit no ka ʻike in vitro qualitative o Klebsiella pneumoniae (KPN), Acinetobacter baumannii (Aba), Pseudomonas aeruginosa (PA) a me ʻehā carbapenem resistance genes (ʻo ia hoʻi ʻo KPC, NDM, OXA48 a me IMP) i nā laʻana sputum kanaka, e hāʻawi. ke kumu o ke alakaʻi ʻana i ka maʻi maʻi, ka mālama ʻana a me ka lāʻau lapaʻau no nā maʻi i manaʻo ʻia he maʻi bacterial.

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

Inoa mea kūʻai

HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii a me Pseudomonas Aeruginosa a me nā Genes Resistance Drug (KPC, NDM, OXA48 a me IMP) Multiplex Detection Kit(Fluorescence PCR)

Palapala

Epidemiology

ʻO Klebsiella pneumoniae kahi pathogen opportunistic maʻamau maʻamau a ʻo kekahi o nā maʻi pathogenic koʻikoʻi e hoʻoulu ai i nā maʻi nosocomial.Ke emi mai ke kū'ē o ke kino, komo ka bacteria i loko o ka māmā mai ka ʻōpū hanu, e hoʻoulu ai i ka maʻi ma nā wahi he nui o ke kino, a ʻo ka hoʻohana mua ʻana i nā lāʻau antibiotic ke kī e ola ai.^[1].

ʻO ka wahi maʻamau o ka maʻi Acinetobacter baumannii ʻo ia ka māmā, he pathogen koʻikoʻi ia no ka maʻi pneumonia i loaʻa i ka Halemai (HAP), ʻoi aku ka maʻi pneumonia pili Ventilator (VAP).Hoʻopili pinepine ʻia me nā maʻi bacterial a me nā fungal ʻē aʻe, me nā hiʻohiʻona o ka nui o ka maʻi maʻi a me ka nui o ka make.

ʻO Pseudomonas aeruginosa ka maʻamau maʻamau o ka gram-negative bacilli non-fermentative i ka hana lapaʻau, a he mea koʻikoʻi opportunistic pathogen no ka maʻi i loaʻa i ka haukapila, me nā ʻano o ka colonization maʻalahi, ka hoʻololi maʻalahi a me ka pale ʻana i nā lāʻau he nui.

Kanal

Inoa	PCR-Mix 1	PCR-Mix 2
Kanal FAM	Aba	IMP
Kaila VIC/HEX	Hoomalu Kuloko	KPC
CY5 Channel	PA	NDM
Kanal ROX	KPN	OXA48

Nā ʻāpana ʻenehana

Waihona	≤-18 ℃
Kau-ola	12 mahina
ʻAno Hoʻohālike	Sputum
Ct	≤36
CV	≤10.0%
LoD	1000 CFU/mL
kikoo	a) Hōʻike ka hoʻāʻo cross-reactivity ʻaʻohe o kēia kit reactivity me nā pathogens hanu ʻē aʻe, e like me Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella Escheudomophila. nā fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus a me ka sputum samples me ka ʻole o nā pahu hopu, etc. b) Hiki ke hoʻopilikia: E koho i ka mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, a me roxithromycin, etc. mai hoʻopilikia i ka ʻike ʻana o Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa a me ka carbapenem resistance genes KPC, NDM, OXA48 a me IMP.
Nā mea hana pili	Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio^®5 Pūnaehana PCR manawa maoli LightCycler^®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Bioer) MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli