ʻO ka maʻi Monkeypox a me ka Typing Nucleic Acid
Inoa huahana
HWTS-OT202-Pahu ʻIke no ka Maʻi Monkeypox a me ke ʻAno Nucleic Acid (Fluorescence PCR)
Palapala hōʻoia
CE
ʻAlapia
He maʻi lele zoonotic ʻoi ka Monkeypox (Mpox) i hoʻokumu ʻia e ka Monkeypox Virus (MPXV). He ʻano poepoe a oval paha ka MPXV, a he maʻi DNA kaula pālua ia me ka lōʻihi o kahi o 197Kb.[1]. Hoʻolaha nui ʻia ka maʻi e nā holoholona, a hiki i nā kānaka ke loaʻa i ka maʻi ma ke nahu ʻia ʻana e nā holoholona i loaʻa i ka maʻi a i ʻole ma ka pili pololei ʻana me ke koko, nā wai kino a me ka ʻulaʻula o nā holoholona i loaʻa i ka maʻi. Hiki nō hoʻi ke hoʻolaha ʻia ka maʻi ma waena o nā kānaka, ma o nā kulu hanu i ka wā o ka launa pū ʻana me ke alo a i ʻole ma o ka pili pololei ʻana me nā wai kino o ka mea maʻi a i ʻole nā mea i haumia ʻia.[2-3]Ua hōʻike ʻia nā haʻawina ua hoʻokumu ʻo MPXV i ʻelua mau clades like ʻole: clade I (i ʻike mua ʻia ʻo ka clade Central African a i ʻole ka clade Congo Basin) a me ka clade II (i kapa ʻia ʻo ka clade West African). Ua hōʻike maopopo ʻia ka mpox o ka clade Congo Basin e hiki ke hoʻoili ma waena o nā kānaka a hiki ke make, ʻoiai ʻo ka mpox o ka clade West African ke kumu o nā hōʻailona ʻoluʻolu a he haʻahaʻa ka nui o ka hoʻoili ʻana mai ke kanaka a i ke kanaka.[4].
ʻAʻole i manaʻo ʻia nā hopena hoʻāʻo o kēia pahu e lilo i mea hōʻailona hoʻokahi no ka ʻike ʻana i ka maʻi MPXV i nā maʻi, pono e hui pū ʻia me nā ʻano lapaʻau o ka mea maʻi a me nā ʻikepili hoʻāʻo hale hana ʻē aʻe e hoʻoholo pololei i ka maʻi o ka pathogen a hoʻokumu i kahi hoʻolālā lapaʻau kūpono e hōʻoia i ka mālama palekana a me ka maikaʻi.
Kanal
| ʻOhana | ʻĀpana MPXV II |
| ROX | ʻO ka waikawa nucleic honua MPXV |
| VIC/HEX | ʻĀpana MPXV I |
| CY5 | ka mana kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | wai ʻili kanaka, nā ʻūpā oropharyngeal a me ke serum |
| Ct | ≤38 (FAM, VIC/HEX, ROX), ≤35（IC) |
| LoD | 200 Kope/mL |
| Nā Mea Hana Pili | Mea hoʻohui ʻike ʻano I: Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®ʻŌnaehana PCR Manawa Maoli 480 Nā ʻōnaehana ʻike PCR manawa maoli ʻo LineGene 9600 Plus (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻopololei Wela Manawa Maoli (Suzhou Molarray Co., Ltd.) ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 Mea hoʻohui ʻike ʻano II: ʻEudemonaTMʻO AIO800 (HWTS-EQ007)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Kahe Hana
