Monkeypox Virus a me ke kikokiko Nucleic Acid
inoa huahana
HWTS-OT202-Monkeypox Virus a me ke kikokiko ʻana i ka pahu ʻike ʻakika nucleic (Fluorescence PCR)
Palapala
CE
Epidemiology
ʻO ka Monkeypox (Mpox) kahi maʻi maʻi maʻi zoonotic i hoʻokumu ʻia e ka Monkeypox Virus (MPXV). ʻO ka MPXV he pōhaku pōʻai a oval paha ke ʻano, a he maʻi maʻi DNA kaulua me ka lōʻihi o 197Kb.[1]. Hoʻouna ʻia ka maʻi e nā holoholona, a hiki i ke kanaka ke loaʻa i ka nahu ʻia e nā holoholona i maʻi ʻia a i ʻole ma ka hoʻopili pololei ʻana i ke koko, ka wai kino a me ka maʻi o nā holoholona i maʻi ʻia. Hiki ke hoʻoili ʻia ka maʻi ma waena o nā kānaka, ma o nā kulu hanu i ka wā lōʻihi, pili pololei i ke alo a i ʻole ma o ka hoʻopili pololei ʻana me nā wai kino o ka mea maʻi a i ʻole nā mea haumia.[2-3]. Ua hōʻike ʻia nā haʻawina e hoʻokumu ʻia ana ka MPXV i ʻelua clade ʻokoʻa: clade I (i ʻike mua ʻia ʻo Central African clade a i ʻole Congo Basin clade) a me clade II (kapa ʻia ma mua ʻo West Africa clade). Ua hōʻike maopopo ʻia ka mpox o Congo Basin clade e hiki ke lawe ʻia ma waena o nā kānaka a hiki ke make, ʻoiai ʻo ka mpox o West Africa clade e hōʻeha i nā hōʻailona maʻalahi a he haʻahaʻa haʻahaʻa o ka hoʻouna ʻana i ke kanaka-i-kanaka.[4].
ʻAʻole i manaʻo ʻia nā hopena hoʻāʻo o kēia kit e lilo i mea hōʻailona hoʻokahi no ka hōʻike ʻana i ka maʻi MPXV i nā maʻi, pono e hui pū ʻia me nā ʻano maʻi o ka mea maʻi a me nā ʻikepili hoʻokolohua ʻē aʻe e hoʻoponopono pono i ka maʻi o ka pathogen a hoʻokumu i kahi hoʻolālā lapaʻau kūpono e hōʻoia i ka mālama palekana a me ka maikaʻi.
Kanal
| FAM | MPXV clade II |
| ROX | MPXV honua nucleic acid |
| VIC/HEX | MPXV papa I |
| CY5 | hoʻomalu kūloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | ʻO ka wai o ke kanaka, nā ʻōpala oropharyngeal a me ka serum |
| Ct | ≤38 (FAM, VIC/HEX, ROX), ≤35（IC) |
| LoD | 200 Kope/mL |
| Nā mea hana pili | ʻAno I ʻike reagent: Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻokele Thermal Cycler Manawa maoli (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli ʻAno ʻike ʻike II: EudemonaTMAIO800 (HWTS-EQ007)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Holo Hana
