ʻO kaʻu Tīwī Myscoctiesis Muscoclosis nukle a me RIFIMICIN ke kū'ē
Hua waihona
Hwts-RT074B-MYCECACTIEIMIMSIIIIM TABERCULOSICSIGCUSCUSCIAL TABICLES ACIDE a me RIFIMICIN CUFTORS CURTOR (melting curve)
HAAKAANA
CE
Epideminey
ʻO TRUBCULSIRSETERSIIII, pōkole e like me ka tuppercle bacillus, tb,ʻo ia ka bucterium pathogenic. I kēia manawa, hoʻohana pinepineʻia nā lāʻau anti-line anti-tuberculosis i loko o ka gonimolinid, amikactinit . ^ E Ha yM. Eia naʻe, ma muli o ka hoʻohanaʻoleʻana o ʻO Anti-Tubercloysis nā lāʻau lapaʻau a me nā hiʻohiʻona o ka pā o ka pā o ka pākekeʻo Tycoblosis
Ua hoʻohana nuiʻiaʻo Rifampicin i ka mālamaʻana i nā maʻi pulmonary ma mua o ka lā 1970s, a loaʻa kahi hopena koʻikoʻi. ʻO ia ka koho mua e hoʻokaʻawale i ka chemotherapy o nā mea maʻi plumonary ʻO ka pale kaua Rifamcicin i kumu nuiʻia e ka mutation o ka gpob gen. ʻOiai e hele mau ana nā lāʻau lapaʻau Anti-tubercloysis e hele mau ana, a me ka maʻi lapaʻau o nā lāʻau lapaʻau lāʻau lāʻau, ua kiʻekiʻeʻo ka pelekikenaʻoi ke kiʻekiʻe. ʻIke maopopo,ʻo ka mea maʻi MyCocouclesis i nā maʻi me ka nui o ka maʻi pulmonary,ʻaʻole e hoʻonui i ka pae o ka makeʻana o ka maʻi maʻi.
Kauwe
Kauwe | Channels a me nā flutorophores | ʻO keʻano he mea nui | Reaction buffer b | Reaction buffer c |
Hauhana Limu | Hōʻike: Han, Queencher:ʻAʻohe | rpob 507-514 | rPob 513-520 | 38KD a he6110 |
Ke KanakaʻoL | Hōʻike: Cy5, Queencher:ʻAʻohe | rPob 520-527 | RPOB 527-533 | / |
Hex (VIC) Channel | Hōʻike: Hex (VIC), Queencher:ʻAʻohe | Kaohi kūloko | Kaohi kūloko | Kaohi kūloko |
Nā poʻoaʻenehana
Huanui | ≤-18 ℃ i ka pōʻeleʻele |
'Āmaʻomaʻo | 12 mau mahina |
ʻAno kiko'ī | Scutumer |
CV | ≤5.0% |
Lolulu | mycpecturitiium trubercolsis 50 bacteria / ml RIFIMICIN-CORSISAT CHATY TYPE: 2x103Bacteria / ml HomozyGous mutant: 2x103Bacteria / ml |
Kiko'ī | ʻIke ia i nā'ōpala maikaʻiʻole-type mysclusis a me nā pūnaewele o nā mea e kū'ē ai i nā gifigickin |
Nā mea pili: | SLAN-96P mea maoli maoli PCR Biorad CFX96 Nā Kūlana Kūlana Lacr ʻO ka'ōnaehana uila |
Nā kahe o ka hana
Inā e hoʻohana i ka macro & micro-micro-set General DNA / RNA Kit (HWTS-3019-9 Nuckac acid extractor (hwts-3006c, hwts-3006b) a iʻole macro & micro-micro-micro-micro Column (hwts-3022-50) e JiangSU Macro & Micro-text CO. hoʻokaʻawale i loko o ka mana kūloko i loko o ka mana kūpono, ka mana maikaʻi a me ke kaʻina hana i hōʻikeʻia e like me nā mea e like me ka extraction Nā'ōkuhi. ʻO ka nui o ka hua'ōlelo i kohoʻia he 200μl, a me ka leo i'ōleloʻiaʻia he 100μl.