ʻO kaʻu Tīwī Myscoctiesis Muscoclosis nukle a me RIFIMICIN ke kū'ē

Kākau pōkole:

He kūpono kēia pahu no kaʻike muaʻana o ka myscocactiurium tna i nā misberculos o ka chotro e pili ana i ka codon codon codto.


Huahana kikoʻipiop

Nā Kūlana Huakaʻi

Hua waihona

Hwts-RT074B-MYCECACTIEIMIMSIIIIM TABERCULOSICSIGCUSCUSCIAL TABICLES ACIDE a me RIFIMICIN CUFTORS CURTOR (melting curve)

HAAKAANA

CE

Epideminey

ʻO TRUBCULSIRSETERSIIII, pōkole e like me ka tuppercle bacillus, tb,ʻo ia ka bucterium pathogenic. I kēia manawa, hoʻohana pinepineʻia nā lāʻau anti-line anti-tuberculosis i loko o ka gonimolinid, amikactinit . ^ E Ha yM. Eia naʻe, ma muli o ka hoʻohanaʻoleʻana o ʻO Anti-Tubercloysis nā lāʻau lapaʻau a me nā hiʻohiʻona o ka pā o ka pā o ka pākekeʻo Tycoblosis

Ua hoʻohana nuiʻiaʻo Rifampicin i ka mālamaʻana i nā maʻi pulmonary ma mua o ka lā 1970s, a loaʻa kahi hopena koʻikoʻi. ʻO ia ka koho mua e hoʻokaʻawale i ka chemotherapy o nā mea maʻi plumonary ʻO ka pale kaua Rifamcicin i kumu nuiʻia e ka mutation o ka gpob gen. ʻOiai e hele mau ana nā lāʻau lapaʻau Anti-tubercloysis e hele mau ana, a me ka maʻi lapaʻau o nā lāʻau lapaʻau lāʻau lāʻau, ua kiʻekiʻeʻo ka pelekikenaʻoi ke kiʻekiʻe. ʻIke maopopo,ʻo ka mea maʻi MyCocouclesis i nā maʻi me ka nui o ka maʻi pulmonary,ʻaʻole e hoʻonui i ka pae o ka makeʻana o ka maʻi maʻi.

Kauwe

Kauwe

Channels a me nā flutorophores

ʻO keʻano he mea nui

Reaction buffer b

Reaction buffer c

Hauhana Limu

Hōʻike: Han, Queencher:ʻAʻohe

rpob 507-514

rPob 513-520

38KD a he6110

Ke KanakaʻoL

Hōʻike: Cy5, Queencher:ʻAʻohe

rPob 520-527

RPOB 527-533

/

Hex (VIC) Channel

Hōʻike: Hex (VIC), Queencher:ʻAʻohe

Kaohi kūloko

Kaohi kūloko

Kaohi kūloko

Nā poʻoaʻenehana

Huanui

≤-18 ℃ i ka pōʻeleʻele

'Āmaʻomaʻo

12 mau mahina

ʻAno kiko'ī

Scutumer

CV

≤5.0%

Lolulu

mycpecturitiium trubercolsis 50 bacteria / ml

RIFIMICIN-CORSISAT CHATY TYPE: 2x103Bacteria / ml

HomozyGous mutant: 2x103Bacteria / ml

Kiko'ī

ʻIke ia i nā'ōpala maikaʻiʻole-type mysclusis a me nā pūnaewele o nā mea e kū'ē ai i nā gifigickin

Nā mea pili:

SLAN-96P mea maoli maoli PCR

Biorad CFX96 Nā Kūlana Kūlana Lacr

ʻO ka'ōnaehana uila

Nā kahe o ka hana

Inā e hoʻohana i ka macro & micro-micro-set General DNA / RNA Kit (HWTS-3019-9 Nuckac acid extractor (hwts-3006c, hwts-3006b) a iʻole macro & micro-micro-micro-micro Column (hwts-3022-50) e JiangSU Macro & Micro-text CO. hoʻokaʻawale i loko o ka mana kūloko i loko o ka mana kūpono, ka mana maikaʻi a me ke kaʻina hana i hōʻikeʻia e like me nā mea e like me ka extraction Nā'ōkuhi. ʻO ka nui o ka hua'ōlelo i kohoʻia he 200μl, a me ka leo i'ōleloʻiaʻia he 100μl.


  • MAUI:
  • ANE:

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