ʻO ka loiloi Traceability mua loa ma muli o ka Genome holoʻokoʻa o Brucella abortus ma ka moku ʻo Zhejiang - ʻO ka hui Bioinformatics mai Macro & Micro-Test e kōkua i ka hoʻopuka hui pū ʻana

—— Haʻawina Hana Pū na Zhejiang CDC, Macro & Micro-Test, a me Kina CDC i Paʻi ʻia ma Frontiers in Cellular and Infection Microbiology

ʻIkepili o ke aʻo ʻana

I Mei 2026, ua paʻi ʻo Frontiers in Cellular and Infection Microbiology (JCR Q1, IF ≈ 4.6) i kahi pepa i alakaʻi ʻia e ke Kikowaena Hoʻomalu a me ka Pale ʻana i nā maʻi o Zhejiang Provincial (Zhejiang CDC), me ka hui bioinformatics mai Beijing Macro & Micro-Test Bio-Tech Co., Ltd. a me ka National Institute for Communicable Disease Control and Prevention (China CDC) ma ke ʻano he mau mea kākau like. ʻO ke poʻo inoa o ke aʻo ʻana:
"Ka ʻike ʻana a me ka loiloi phylogenetic o ʻehiku mau ʻano Brucella abortus ma Zhejiang, Kina."

ʻO kēia haʻawina ke ʻano mua o ka loiloi phylogenetic traceability ʻōnaehana holoʻokoʻa o Brucella abortus (B. abortus) ma ka Panalāʻau ʻo Zhejiang, Kina. Ua kālailai ke kime i ʻehiku mau isolates i hōʻiliʻili ʻia mai 2015 a i 2025 (ʻehā mau ʻano mai ke kanaka a me ʻekolu mau ʻano mai ka bipi mai Jinhua, Quzhou, a me Ningbo). Hāʻawi nā ʻike i nā hōʻike genomic no ke kumu a me nā ala hoʻoili o kēia "ʻano dominant ʻākau" ma kahi ʻāina ahulau hema atypical o ka hikina o Kina.

Ka Moolelo a me ke Koʻikoʻi

He maʻi zoonotic ka Brucellosis i hoʻokumu ʻia e nā bacteria o ka genus Brucella. Hoʻopilikia nui ʻo Brucella abortus i nā bipi akā hiki ke hoʻoulu i nā maʻi i nā kānaka. Ma Kina, hōʻike ka brucellosis i kahi ʻokoʻa ʻāina: ʻo ka nui o ka hanana ma nā panalāʻau ʻākau (e like me Inner Mongolia, Shanxi, Heilongjiang). I ka hoʻohālikelike ʻana, ʻo nā panalāʻau hema, me Zhejiang, ua noho aliʻi ʻia e Brucella melitensis i ka mōʻaukala, me ka liʻiliʻi o nā hihia i hōʻike ʻia o B. abortus. ʻO kēia ʻokoʻa ʻāina e hoʻolilo i ke ʻano genetic a me ka huli ʻana i ke kumu o B. abortus ma Zhejiang i mea nui i ke olakino lehulehu.

Nā Hana a me nā ʻIke Koʻikoʻi

Ua hoʻohana ka hui noiʻi i kahi hoʻolālā multi-pronged e hoʻohui ana i ka biology molekala a me ka bioinformatics:

1.Ka ʻike ʻana i ka pathogen a me ke ʻano maʻamau

Ua hōʻoia ka BCSP-31 gene PCR a me AMOS-PCR ua lilo nā isolates ʻehiku a pau i B. abortus.

Ua hōʻike ka Multilocus sequence typing (MLST) ma muli o ʻeiwa mau genes housekeeping no ke ʻano sequence ST2 nā isolates āpau, e hōʻike ana i ka homogeneity genetic kiʻekiʻe ma waena o nā ʻano B. abortus e kaapuni ana ma Zhejiang.

2.Ka wehewehe ʻana o ka genome holoʻokoʻa

Ua hana ʻia ke kaʻina hana genome holoʻokoʻa ma ka paepae Illumina NovaSeq. Ua hōʻike ka loiloi nucleotide identity awelika (ANI) ua kaʻana like nā isolates Zhejiang a hiki i ka 99.99% like me ke ʻano kuhikuhi B. abortus 544.

Ua hōʻike ka loiloi pan-genome i kahi heluna kanaka i mālama nui ʻia: ua ʻike ʻia he 3,084 mau genes koʻikoʻi, me 10 mau genes pūpū wale nō, a ʻaʻohe genes koʻikoʻi palupalu a i ʻole nā ​​​​genes ao i ʻike ʻia.

3.Nā ʻikepili gene virulence a me ke kū'ē antimicrobial

Ua wānana ʻia he 68 mau mea e pili ana i ka virulence, e uhi ana i nā ala kuʻuna e like me ka biosynthesis LPS, ka ʻōnaehana huna T4SS, a me ka ʻōnaehana hoʻoponopono ʻelua-ʻāpana BvrR-BvrS. ʻO ka mea nui, ʻaʻohe o nā isolates āpau i nā genes adhesin bmaA a me btaF. Ua ʻike wale ka loiloi gene kū'ē i ka gene mprF i loko o ka waihona ʻikepili CARD, me ka ʻole o nā mea hoʻoholo kū'ē ʻē aʻe i ʻike ʻia.

4. Ke kūkulu hou ʻana o ka phylogenetic a me ka hahai ʻana i ka hoʻoili ʻana

Ua kau ka loiloi Core-genome single-nucleotide polymorphism (cgSNP) i nā isolates Zhejiang ma kahi kūlana kikoʻī ma ka lāʻau phylogenetic honua. Ua hōʻike nā hopena ua hoʻokumu nā ʻano Zhejiang i kahi hui monophyletic me nā ʻano mai Rusia, Mongolia, a me kekahi mau panalāʻau ʻākau o Kina (Ningxia, Heilongjiang, Inner Mongolia, Hebei, Gansu, Beijing). Ua māhele hou ʻia kēia hui i ʻekolu mau sub clades like ʻole (Clade 1-3), e hōʻike ana i nā hanana hoʻolauna kūʻokoʻa he nui.

Nā Hopena a me nā Manaʻo

Hāʻawi kēia haʻawina i ka ʻikepili genomic mua loa o B. abortus ma ka moku ʻo Zhejiang a loaʻa mai kekahi mau hopena koʻikoʻi:

1. ʻOlear ʻikepili hoʻoilina– No ST2 nā ʻano B. abortus e hoʻopuni ana ma Zhejiang, ua mālama nui ʻia ka genomics, a ke hōʻike nei i kahi moʻokūʻauhau brucellosis bovine maʻamau.

2. ʻEvika paʻa o ka hoʻoili ʻana ma waena o nā ʻāpana– ʻAʻole kākoʻo ka loiloi phylogenetic i ke ola ʻana o kahi moʻokūʻauhau kūʻokoʻa ma Zhejiang. Akā, hōʻike ikaika ka ʻikepili ua puka mai kēia mau ʻano mai ka ʻākau o Kina a hiki ke kaʻana like i kahi ʻano evolutionary like me nā ʻano mai Rusia a me Mongolia. ʻO ke alo o ʻekolu mau sub clades e hōʻike ana i nā hanana hoʻolauna kaʻawale he nui.

3. Nā hopena olakino lehulehu– Hōʻike nā ʻike i ka waiwai o ka nānā ʻana i ka genomic no ka brucellosis ma nā wahi i ʻole endemic maʻamau e like me Zhejiang. ʻOiai he haʻahaʻa ka helu o nā hihia i kēia manawa, hiki i nā mea hana hoʻonā kiʻekiʻe e like me cgSNP ke ʻimi pono i ke kumu o nā maʻi ahulau i lawe ʻia mai a hāʻawi i nā hōʻike ʻepekema e hoʻopau i nā kaulahao hoʻoili e pili ana me ka lawe ʻana i nā holoholona ma waena o nā panalāʻau.

ʻAʻole wale kēia hana e hoʻopiha i kahi hakahaka noiʻi ma ka Panalāʻau ʻo Zhejiang akā hāʻawi pū kekahi i ka ʻikepili kumu hou no ka nānā ʻana i nā pathogen a me ka loiloi pilikia o ka brucellosis ma ka ʻāpana ʻo Yangtze River Delta.

ʻIke Pepa:
Yang, Y., Shi, X., Chen, J., Wang, L., Wu, Z., Yao, W., … & Wu, B. (2026). Ka ʻike ʻana a me ka loiloi phylogenetic o ʻehiku mau ʻano Brucella abortus ma Zhejiang, Kina. Frontiers in Cellular and Infection Microbiology, 16, 1758965.