Orientia tsutsugamushi Nucleic Acid
inoa huahana
HWTS-OT002-Orientia tsutsugamushi Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
ʻO ka scrub typhus kahi maʻi febrile koʻikoʻi i hoʻokumu ʻia e ka maʻi Orientia tsutsugamushi (Ot). ʻO ka Orientia scrub typhus kahi mea ʻokoʻa Gram-negative obligate intracellular parasitic microorganism. Aia ka Orientia scrub typhus i ka genus Orientia ma ke kauoha Rickettsiales, ka ʻohana Rickettsiaceae, a me ka genus Orientia. Hoʻouna ʻia ka scrub typhus ma o ka nahu ʻana o nā larvae chigger e lawe ana i nā pathogens. Ua hōʻike ʻia e ke kuni kiʻekiʻe koke, eschar, lymphadenopathy, hepatosplenomegaly, a me ka leukopenia koko peripheral, a me nā mea ʻē aʻe.
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | nā laʻana serum |
| CV | ≤5.0% |
| LoD | 500 kope/μL |
| Nā mea hana pili | Hoʻohana ʻia i ka ʻano I detection reagent: Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli, QuantStudio®5 Pūnaehana PCR manawa maoli, SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Pūnaehana PCR manawa maoli, BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli. E pili ana i ke ʻano II detection reagent: EudemonaTMAIO800 (HWTS-EQ007) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Holo Hana
ʻO Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hana ʻia ka unuhi e like me ke kuhikuhi no ka hoʻohana ʻana i kēia reagent extraction. ʻO 200μL ka nui o ka laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 100μL.
