SARS-CoV-2/maʻi palū A/maʻi palū B

Kiʻi Hoʻohanohano ʻia ʻo SARS-CoV-2/influenza A /influenza B

Wehewehe Pōkole:

He kūpono kēia pahu no ka ʻike ʻana i ka SARS-CoV-2, ka maʻi influenza A a me ka maʻi influenza B nucleic acid o nā laʻana swab nasopharyngeal a me nā laʻana swab oropharyngeal o ka poʻe i manaʻo ʻia he maʻi SARS-CoV-2, influenza A a me ka maʻi influenza B. Hiki ke hoʻohana ʻia i nā hihia pneumonia i manaʻo ʻia a me nā hihia cluster i manaʻo ʻia a no ka ʻike ʻana a me ka ʻike ʻana i ka SARS-CoV-2, influenza A a me ka maʻi influenza B nucleic acid i nā laʻana swab nasopharyngeal a me nā laʻana swab oropharyngeal o ka maʻi Coronavirus hou i nā kūlana ʻē aʻe.

E leka uila mai iā mākou

Nā kikoʻī huahana

Nā Lepili Huahana

Inoa huahana

Pahu ʻIke Hui Pū ʻia ʻo HWTS-RT148-SARS-CoV-2/influenza A/influenza B Nucleic Acid (Fluorescence PCR)

Kanal

Inoa Kanal	PCR-Mix 1	PCR-Mix 2
Kanal FAM	ʻO ka gene ORF1ab	ʻIVA
Kahawai VIC/HEX	Ka mana kūloko	Ka mana kūloko
Kanal CY5	ʻInoa N	/
Kanal ROX	ʻO ke kine E	IVB

Nā Palena ʻenehana

Ka Waihona	-18℃
Ola papa	12 mahina
ʻAno Laʻana	nā ʻūpā nasopharyngeal a me nā ʻūpā oropharyngeal
Pahuhopu	ʻEkolu mau pahuhopu SARS-CoV-2 (nā genes Orf1ab, N a me E)/influenza A/influenza B
Ct	≤38
CV	≤10.0%
LoD	SARS-CoV-2：300 Kope/mL maʻi influenza A：500 Kope/mL maʻi influenza B：500 Kope/mL
Kūlana kikoʻī	a) Ua hōʻike ʻia nā hopena hoʻāʻo kea ua kūpono ka pahu me ka coronavirus kanaka SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, ka maʻi respiratory syncytial A a me B, ka maʻi parainfluenza 1, 2 a me 3, rhinovirusA, B a me C, adenovirus 1, 2, 3, 4, 5, 7 a me 55, ka metapneumovirus kanaka, enterovirus A, B, C a me D, ka maʻi pulmonary cytoplasmic kanaka, ka maʻi EB, ka maʻi measles Human cytomegalovirus, rotavirus, norovirus, ka maʻi mumps, ka maʻi varicella zoster, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata ʻAʻohe hopena keʻa ma waena o Pneumocystis yersini lāua ʻo Cryptococcus neoformans. b) Ka hiki ke pale i ke komo ʻana: koho i ka mucin (60mg/mL), 10% (V/ V) koko kanaka, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), sodium chloride (loaʻa ka mea mālama) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisone (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), pramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceprotene (40μg/mL) Meropenem (200mg/mL), levofloxacin (10μg/mL) a me tobramycin (0.6mg/mL). Ua hōʻike nā hopena ʻaʻohe pane hoʻopilikia o nā mea hoʻopilikia ma nā pae i luna i nā hopena ʻike o nā pathogens.
Nā Mea Hana Pili	Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 SLAN^®Nā ʻōnaehana PCR Manawa Maoli -96P Nā ʻōnaehana PCR Manawa Maoli QuantStudio™ 5 LightCycler^®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96