14 Nā ʻano o ka maʻi papilloma kanaka kiʻekiʻe (16/18/52 kikokiko)
Inoa mea kūʻai
HWTS-CC019A-14 Nā ʻano o ka maʻi papilloma kanaka kiʻekiʻe (16/18/52 ʻano) pahu ʻike ʻakika nucleic (Fluorescence PCR)
Epidemiology
Ua hōʻike ʻia nā haʻawina ʻo nā maʻi hoʻomau HPV a me nā maʻi he nui kekahi o nā kumu nui o ka maʻi kanesa cervical.I kēia manawa, ʻaʻole i ʻike ʻia nā lāʻau lapaʻau maikaʻi no ka maʻi maʻi ʻāʻī i hoʻokumu ʻia e ka HPV.He mea koʻikoʻi ka hoʻokumu ʻana i kahi hoʻāʻo diagnostic etiology maʻalahi, kikoʻī a wikiwiki hoʻi no ka maʻi maʻi a me ka mālama ʻana i ka maʻi maʻi cervical.
Kanal
| Kanal | ʻAno |
| FAM | HPV 18 |
| VIC/HEX | HPV 16 |
| ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY5 | HPV 52 |
| Quasar 705/CY5.5 | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | ʻUrine, ʻAka Puʻupuʻu, ʻAka Puka |
| Ct | ≤28 |
| LoD | 300 kope/mL |
| kikoo | ʻAʻohe hoʻoikaika ʻana me nā laʻana hanu ʻē aʻe e like me Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumovirus A1/A2 B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. a me DNA genomic kanaka. |
| Nā mea hana pili | MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli (Suzhou Molarray Co., Ltd.) ʻO BioRad CFX96 Pūnaehana PCR manawa maoli a me BioRad CFX Opus 96 Pūnaehana PCR manawa maoli. |
Holo Hana
1.Laʻana mimi
A: Lawe1.4mL o ka mimi e ho'āʻo a centrifuge ma 12000rpm no 5 minuke;e hoʻolei i ka supernatant (pono e mālama i ka 10-20μL supernatant mai ka lalo o ka centrifuge tube), e hoʻohui i 200μL o ka hāpana hoʻokuʻu reagent, a pono e alakaʻi ʻia ka unuhi hope e like me nā kuhikuhi no ka hoʻohana ʻana i ka Macro & Micro-Test Sample Release Mea hoʻoponopono (HWTS-3005-8).
B: Lawe1.4mL o ka mimi e ho'āʻo a centrifuge ma 12,000rpm no 5 mau minuke;e hoʻolei i ka supernatant (ua paipai ʻia e mālama i ka 10-20μL o ka supernatant mai ka lalo o ka pahu centrifuge), a hoʻohui i 200μL o ka saline maʻamau e hoʻomaha hou, e like me ka hāpana e hoʻāʻo ʻia.Hiki ke alakaʻi ʻia ka unuhi ʻana me ka Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & ʻO ka Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. e like me ka ʻōlelo aʻo.s no ka hoohana ana.ʻO ka nui o ka elution i manaʻo ʻia he 80μL.
C: Lawe1.4mL o ka mimi e ho'āʻo a centrifuge ma 12,000rpm no 5 mau minuke;e hoʻolei i ka supernatant (ua paipai ʻia e mālama i ka 10-20μL o ka supernatant mai ka lalo o ka pahu centrifuge), a hoʻohui i 200μL o ka saline maʻamau e hoʻomaha hou, e like me ka hāpana e hoʻāʻo ʻia.Hiki ke hoʻokō ʻia ka unuhi ʻana ma hope meQIAamp DNA Mini Kit(51304) na QIAGEN a i ʻole Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Pono e hana ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana.ʻO 200 ka nui o ka laʻanaμL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80μL.
2. He laʻana swab cervical/vaginal swab
A: E lawe i 1mL o ka hāpana e hoʻāʻo ʻia ma kahi 1.5mLof paipu centrifuge,acentrifuge ma 12000rpm no 5 mau minuke. Discard ka supernatant (manaʻo ʻia e mālama i ka 10-20μL o ka supernatant mai ka lalo o ka pahu centrifuge), e hoʻohui i ka 100μL o ka mea hoʻokuʻu hāpana, a laila unuhi e like me nā kuhikuhi no ka hoʻohana ʻana i ka Macro & Micro-Test Sample Release Reagent ( HWTS-3005-8).
B: Hiki ke alakaʻi ʻia ka unuhi me Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. e like me nā kuhikuhi no ka hoʻohana.ʻO 200μL ka nui o ka laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 80μL.
C: Hiki ke hana ʻia ka unuhi me QIAamp DNA Mini Kit(51304) e QIAGEN a i ʻole Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Pono e hana ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana.ʻO 200 μL ka nui o ka laʻana unuhi, a ʻo ka nui elution i ʻōlelo ʻia80 μL.
3、Ka Puka Puka/ ʻAi ʻApe
Ma mua o ka laʻana, e hoʻohana i ka pulupulu pulupulu e holoi mālie i nā mea huna mai ka pūpū, a e hoʻohana i kahi pulupulu ʻē aʻe i hoʻokomo ʻia me ka hopena mālama cell a i ʻole ka cervical exfoliated cell sampling brush e pili i ka mucosa cervical a huli i ka manawa 3-5 puni e loaʻa ai. pūpū exfoliated pūpū.Wehe mālie i ka pulupulu a i ʻole ka pulupulu,ae hoʻokomo i loko o kahi pahu hāpana me 1mL o ka paʻakai maʻamau sterile. Ama hope o ka holoi ʻana, e ʻomi i ka pulupulu pulupulu a i ʻole ka pulupulu i ka paia o ka paipu a e hoʻolei, e hoʻopaʻa i ka pāpale paipu, a e kaha i ka inoa hāpana (a i ʻole ka helu) a paʻi ma ka pahu hāpana.
