17 Nā ʻano o ka HPV (16/18/6/11/44 Ke kikokiko ʻana)
inoa huahana
HWTS-CC015 17 Nā ʻano o ka Human Papillomavirus (16/18/6/11/44 Type) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi ʻaʻai ʻāʻī kekahi o nā maʻi maʻi maʻamau i ka ʻāpana hānau wahine. Ua hōʻike ʻia ʻo ka maʻi HPV mau a me nā maʻi he nui kekahi o nā kumu nui o ka maʻi maʻi ʻāʻī. I kēia manawa aia ka nele o nā lāʻau lapaʻau maikaʻi i ʻae ʻia no ka maʻi maʻi ʻāʻī ma muli o ka HPV. No laila, ʻo ka ʻike mua ʻana a me ka pale ʻana i ka maʻi ʻāʻī o ka maʻi i hoʻokumu ʻia e ka HPV ʻo ia ke kī i ka pale ʻana i ka maʻi kanesa cervical. ʻO ka hoʻokumu ʻana i nā hoʻokolohua maʻalahi, kikoʻī a wikiwiki hoʻi no nā pathogens he mea koʻikoʻi nui ia no ka hōʻoia ʻana i ka maʻi maʻi cervical.
Kanal
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Ka hooponopono kuloko |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Ka hooponopono kuloko |
Nā ʻāpana ʻenehana
| Waihona | -18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | Laʻana mimi, laʻana ʻaʻahu ʻāʻī o ka ʻōpū o ka wahine, kahi laʻana swab wahine |
| Ct | ≤28 |
| LoD | 300 kope/mL |
| kikoo | ʻAʻohe hoʻololi keʻa me Ureaplasma urealyticum, Chlamydia trachomatis o ka ʻāpana hānau, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold , Gardnerella a me nā ʻano HPV ʻē aʻe ʻaʻole i uhi ʻia e ka kit. |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR Manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Manaʻo ʻia ka hoʻoneʻe ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (000HWC Acid Extractor). HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Hoʻohui i 200μL o ka paʻakai maʻamau e hoʻokuʻu hou i ka pellet ma ka pae 2.1, a laila pono e hana ʻia ka unuhi e like me nā kuhikuhi no ka hoʻohana ʻana i kēia reagent extraction. ʻO ka nui o ka elution i manaʻo ʻia he 80μL.
Manaʻo ʻia ka mea hoʻoheheʻe: QIAamp DNA Mini Kit (51304) a i ʻole Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). E hoʻohui i 200μL o ka paʻakai maʻamau e hoʻokuʻu hou i ka pellet ma ka pae 2.1, a laila pono e hoʻokō ʻia ka unuhi e like me nā kuhikuhi no ka hoʻohana ʻana i kēia reagent. He 200μL ka nui o nā laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 100μL.
Manaʻo ʻia ka mea hoʻoheheʻe: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). E hoʻohui i 200μL o ka laʻana hoʻokuʻu reagent e hoʻokuʻu hou i ka pellet ma ka pae 2.1, a laila pono e hana ʻia ka unuhi e like me nā kuhikuhi no ka hoʻohana ʻana i kēia reagent.
