17 ʻAno o ka HPV (16/18/6/11/44 Typing)
Inoa huahana
HWTS-CC015 17 ʻAno o ka Human Papillomavirus (16/18/6/11/44 Typing) Pahu ʻIke Waikawa Nucleic (Fluorescence PCR)
ʻAlapia
ʻO ke kanesa ʻōpū kekahi o nā puʻupuʻu ʻino maʻamau i loko o ke ala hānau wahine. Ua hōʻike ʻia ʻo ka maʻi HPV mau a me nā maʻi he nui kekahi o nā kumu nui o ke kanesa ʻōpū. I kēia manawa, ʻaʻohe mau lāʻau lapaʻau kūpono i ʻae ʻia no ke kanesa ʻōpū i hoʻokumu ʻia e HPV. No laila, ʻo ka ʻike mua ʻana a me ka pale ʻana i ka maʻi ʻōpū i hoʻokumu ʻia e HPV nā kī i ka pale ʻana i ke kanesa ʻōpū. ʻO ka hoʻokumu ʻana i nā hoʻokolohua diagnostic maʻalahi, kikoʻī a wikiwiki hoʻi no nā pathogens he mea nui loa ia no ka ʻike lapaʻau o ke kanesa ʻōpū.
Kanal
| PCR-Mix1 | ʻOhana | 18 |
| VIC/HEX | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Ka mana kūloko |
| PCR-Mix2 | ʻOhana | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Ka mana kūloko |
Nā Palena ʻenehana
| Ka Waihona | -18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | Hāʻina mimi, hāʻina swab cervical wahine, hāʻina swab vaginal wahine |
| Ct | ≤28 |
| LoD | 300 Kope/mL |
| Kūlana kikoʻī | ʻAʻohe cross-reactivity me Ureaplasma urealyticum, Chlamydia trachomatis of reproductive tract, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella a me nā ʻano HPV ʻē aʻe i uhi ʻole ʻia e ka pahu. |
| Nā Mea Hana Pili | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P LightCycler®480 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana ʻike PCR manawa maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Mea hoʻohuihui i manaʻo ʻia: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E hoʻohui i 200μL o ka saline maʻamau e hoʻokuʻu hou i ka pellet ma ka hana 2.1, a laila e hana ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana i kēia mea hoʻohuihui. ʻO ka nui o ka elution i manaʻo ʻia he 80μL.
ʻO ka mea hoʻohuihui i ʻōlelo ʻia: QIAamp DNA Mini Kit (51304) a i ʻole Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). E hoʻohui i 200μL o ka saline maʻamau e hoʻokuʻu hou i ka pellet ma ka hana 2.1, a laila pono e hana ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana i kēia mea hoʻohuihui. ʻO ka nui o ka laʻana i unuhi ʻia o nā laʻana he 200μL a pau, a ʻo ka nui o ka elution i ʻōlelo ʻia he 100μL.
ʻO ka mea hoʻoheheʻe i ʻōlelo ʻia: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). E hoʻohui i 200μL o ka mea hoʻoheheʻe laʻana e hoʻokuʻu hou i ka pellet ma ka hana 2.1, a laila e hana ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana i kēia mea hoʻoheheʻe.
