Mycobacterium Tuberculosis Nucleic Acid a me Rifampicin(RIF)，Isoniazid Resistance(INH)

ʻO ka wehewehe pōkole:

He kūpono kēia huahana no ka ʻike qualitative o Mycobacterium tuberculosis DNA i loko o nā ʻāpana sputum kanaka i loko o ka vitro, a me ka homozygous mutation ma ka 507-533 amino acid codon region (81bp, rifampicin resistance determining region) o ka rpoB gene e kumu ai ka Mycobacterium tuberculosis kū'ē rifampicin.

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

Inoa mea kūʻai

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), Isoniazid Resistance (INH) Detection Kit (Melting Curve)

Epidemiology

ʻO ka Mycobacterium tuberculosis, i kapa koke ʻia ʻo Tubercle bacillus (TB), ʻo ia ka maʻi maʻi pathogenic e hoʻoulu ai i ka maʻi maʻi.I kēia manawa, ʻo nā lāʻau anti-tuberculosis laina mua i hoʻohana pinepine ʻia me isoniazid, rifampicin a me ethambutol, a me nā mea ʻē aʻe. Eia nō naʻe, ma muli o ka hoʻohana hewa ʻana i nā lāʻau anti-tuberculosis a me nā ʻano o ke ʻano o ka pā hale o ka mycobacterium tuberculosis, ua hoʻomohala ka mycobacterium tuberculosis i ka lāʻau kūʻē i nā lāʻau anti-tuberculosis, kahi e lawe mai ai i nā pilikia koʻikoʻi i ka pale ʻana a me ka mālama ʻana i ka maʻi maʻi.

Kanal

Inoa Pahuhi			Mea hoolaha	Quencher
Paʻa PaʻaA	Paʻa PaʻaB	Paʻa PaʻaC	Mea hoolaha	Quencher
rpoB 507-514	rpoB 513-520	IS6110	FAM	ʻAʻohe
rpoB 520-527	rpoB 527-533	/	CY5	ʻAʻohe
/	/	Ka hooponopono kuloko	HEX(VIC)	ʻAʻohe

Paʻa PaʻaD	Mea hoolaha	Quencher
Māhele hoʻolaha InhA -15C>T, -8T>A, -8T>C	FAM	ʻAʻohe
KatG 315 codon 315G>A、315G>C	CY5	ʻAʻohe
ʻĀpana hoʻolaha AhpC -12C>T, -6G>A	ROX	ʻAʻohe

Nā ʻāpana ʻenehana

Waihona	≤-18 ℃
Kau-ola	12 mahina
ʻAno Hoʻohālike	ʻūhā
CV	≤5.0%
LoD	He 50 bacteria/mL ka LoD o ka mycobacterium tuberculosis.ʻO ka LoD o ka rifampicin-resistant wild type national reference he 2×10³bacteria/mL, a ʻo ka LoD o ke ʻano mutant he 2×10³bacteria/mL.He 2x10 ka LoD o ka ma'i bacteria pale isoniazid-ano hihiu³bacteria/mL, a he 2x10 ka LoD o ka bacteria mutant³bacteria/mL.
kikoo	Ua hōʻike ʻia nā hopena hoʻāʻo keʻa ʻaʻohe hopena keʻa i ka ʻike ʻana i ka genome kanaka, nā mycobacteria non tuberculosis a me nā pathogens pneumonia me kēia pahu;ʻAʻole i ʻike ʻia ka hopena kea ma nā wahi hoʻololi o nā genes kūʻē i ka lāʻau i loko o ka Mycobacterium tuberculosis ʻano hihiu.
Nā mea hana pili	SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Pūnaehana PCR manawa maoli, ʻenehana Hangzhou Bioer QuantGene 9600 Pūnaehana PCR manawa maoli, QuantStudio^®5 Pūnaehana PCR manawa maoli.