ʻEhā ʻano o nā mea hoʻomaʻi hanu nucleic acid
Inoa mea kūʻai
HWTS-RT075-Hoʻomaloʻo-hoʻohuʻu 4 ʻano o nā mea hoʻomaʻi hanu nucleic acid Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi maʻi ʻo Corona Virus 2019, i kapa ʻia ʻo "COVID-19", e pili ana i ka maʻi pneumonia i hoʻokumu ʻia e ka maʻi SARS-CoV-2.ʻO SARS-CoV-2 kahi coronavirus pili i ka genus β.ʻO COVID-19 kahi maʻi maʻi ʻeha hanu, a maʻalahi ka heluna kanaka.I kēia manawa, ʻo ke kumu o ka maʻi ka nui o nā maʻi i loaʻa i ka maʻi e SARS-CoV-2, a ʻo ka poʻe maʻi asymptomatic paha ke kumu o ka maʻi.Ma muli o ka noiʻi epidemiological o kēia manawa, ʻo ka manawa incubation he 1-14 mau lā, ʻo ka hapa nui he 3-7 mau lā.ʻO ke kuni, ka ʻū maloʻo a me ka luhi nā hōʻike nui.Ua loaʻa i kekahi mau maʻi nā hōʻailona e like me ka nasal congestion, runny ihu, ʻeha ʻāʻī, myalgia a me ka diarrhea, etc.
ʻO ka maʻi influenza, i ʻike nui ʻia ʻo "flu", he maʻi ʻeha ʻeha i hoʻokumu ʻia e ka maʻi maʻi influenza.He ma'i lele loa.Hoʻouna nui ʻia ia e ka ʻu ʻana a me ke kihe ʻana.He mea maʻamau i ka pūnāwai a me ka hoʻoilo.Hoʻokaʻawale ʻia nā maʻi maʻi maʻi influenza A (IFV A), influenza B (IFV B), a me ka Influenza C (IFV C) ʻekolu ʻano, no ka maʻi maʻi sticky, kumu nui o ke kanaka no nā maʻi influenza A a me B, hoʻokahi ia. - stranded, ʻāpana RNA virus.ʻO ka maʻi Influenza A he maʻi hanu nui, e like me H1N1, H3N2 a me nā ʻano subtype ʻē aʻe, hiki ke hoʻololi a maʻi i ka honua holoʻokoʻa.ʻO ka "Shift" e pili ana i ka hoʻololi ʻana o ka maʻi maʻi influenza A, ka hopena i ka puka ʻana mai o kahi maʻi hou "subtype".Ua māhele ʻia nā maʻi maʻi influenza B i ʻelua laina, ʻo Yamagata a me Victoria.ʻO ka maʻi Influenza B wale nō ka antigenic drift, a pale aku i ka nānā ʻana a me ka hoʻopau ʻana o ka ʻōnaehana pale kanaka ma o kona hoʻololi ʻana.Eia naʻe, ʻoi aku ka lohi o ka hoʻololi ʻana o ka maʻi maʻi influenza B ma mua o ka maʻi influenza A kanaka.Hiki i ka ma'i Influenza B ke ho'oulu i ka ma'i hanu kanaka a alaka'i i ka ma'i ahulau.
ʻO ka maʻi maʻi hanu hanu (RSV) he maʻi RNA, no ka ʻohana paramyxoviridae.Hoʻouna ʻia ia e nā kulu lewa a me ka pilina pili a ʻo ia ka pathogen nui o ka maʻi ʻeha haʻahaʻa o ka ʻōpū o nā pēpē.Hiki i nā kamaliʻi i loaʻa i ka RSV ke hoʻomohala i ka bronchiolitis koʻikoʻi a me ka maʻi pneumonia, pili i ka hānō i nā keiki.Loaʻa i nā pēpē nā hōʻailona koʻikoʻi, e like me ke kuni kiʻekiʻe, rhinitis, pharyngitis a me ka laryngitis, a laila bronchiolitis a me ka maʻi pneumonia.Hiki ke paʻakikī kekahi mau keiki maʻi me ka otitis media, pleurisy a me ka myocarditis, a me nā mea ʻē aʻe.
Kanal
FAM | SARS-CoV-2 |
VIC(HEX) | RSV |
CY5 | IFV A |
ROX | IFV B |
NED | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
Waihona | 2-8°C |
Kau-ola | 12 mahina |
ʻAno Hoʻohālike | ʻOropharyngeal swab |
Ct | ≤38 |
LoD | SARS-CoV-2: 150 Kope/mL ʻO ka maʻi maʻi Influenza A/Maʻi maʻi influenza B/Maʻi hoʻomaʻamaʻa hanu: 300 Kopi/mL |
kikoo | ʻAʻohe ʻano hoʻopaʻapaʻa me ka coronavirus kanaka SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus kanaka, enterovirus A, B, C, D. staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci a me cryptococcus hānau hou a me ke kanaka genomic nucleic acid. |
Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli, Pūnaehana PCR i hoʻohana ʻia 7500 wikiwiki loa, ʻo QuantStudio®5 Pūnaehana PCR manawa maoli. |
Holo Hana
Koho 1.
Manaʻo ʻia ka hoʻoneʻe ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Koho 2.
Manaʻo ʻia ka mea hoʻoneʻe ʻoki: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP302) na Tiangen Biotech(Beijing) Co., Ltd.