4 Nā ʻano o nā maʻi hoʻomake hanu
inoa huahana
HWTS-RT099- 4 ʻano o nā mea hoʻomaʻi hanu nucleic acid detection kits (Fluorescence PCR)-内参是NED-ABI 7500 Pūnaehana PCR manawa maoli./ABI 7500 ʻO nā Pūnaehana PCR ʻOiaʻiʻo wikiwiki/QuantStudio®5 Pūnaehana PCR manawa maoli
HWTS-RT158-4 Nā ʻano o nā mea hoʻomaʻi hanu Nucleic Acid Detection Kit (Fluorescence PCR)-内参Quasar 705
Epidemiology
ʻO ka maʻi maʻi ʻo Corona Virus 2019, i kapa ʻia ʻo "COVID-19", e pili ana i ka maʻi pneumonia i hoʻokumu ʻia e2019-nCoVmaʻi maʻi.2019-nCoVhe coronavirus no ka β genus. ʻO COVID-19 kahi maʻi maʻi ʻeha hanu, a maʻalahi ka heluna kanaka. I kēia manawa, ʻo ke kumu o ka maʻi ka nui o nā maʻi i loaʻa i ka maʻi2019-nCoV, a ʻo ka poʻe maʻi asymptomatic hiki ke lilo i kumu o ka maʻi. Ma muli o ka noiʻi epidemiological o kēia manawa, ʻo ka manawa incubation he 1-14 mau lā, ʻo ka hapa nui he 3-7 mau lā. ʻO ke kuni, ka ʻū maloʻo a me ka luhi nā hōʻike nui. Ua loaʻa i kekahi mau maʻi ka hōʻailonas like meka ʻāʻī o ka ihu, ka ihu kahe, ka ʻeha ʻāʻī, ka myalgia a me ka ʻōpū, etc.
Kanal
| FAM | 2019-nʻO CoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | -18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | ʻOropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300 kope/mLʻO ka maʻi maʻi Influenza A/Maʻi maʻi influenza B/Maʻi hoʻomaʻamaʻa hanu: 500 Kopi/mL |
| kikoo | a) Hōʻike nā hualoaʻa cross-reactivity ʻaʻohe hopena keʻa ma waena o ka kit a me ka coronavirus kanaka SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, humanydia me ka maʻi pneumonia, humanydia enterovirus. B, C, D, ma'i pulmonary kanaka, ma'i ma'i epstein-barr, ma'i ma'i 'a'ai, ma'i ma'i cytomegalo kanaka, rotavirus, norovirus, ma'i parotitis, ma'i varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus, pnemonia pnemonia, tuberculosis. uahi aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci a me cryptococcus hānau hou a me ke kanaka genomic nucleic acid. b) Hiki ke hoʻopilikia: koho i ka mucin (60mg / mL), 10% (v / v) o ke koko a me ka phenylephrine (2mg / mL), oxymetazoline (2mg / mL), sodium chloride (me nā preservatives) (20 mg / mL), beclomethasone (20mg / mL), dexanisg/mL (20mg / mL), dexamgolimethasone (2) triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), ribavirin (10mg/mL) (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) a me ka ho'āʻo. Hōʻike nā hualoaʻa ʻaʻohe hopena hoʻopilikia i nā hopena hoʻāʻo o nā pathogens. |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoliHoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
ʻO Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) i hana ʻia e Jiangsu Macro & Micro-Test Med-Tech 0μlu ka laʻana ʻo ia ka volume. ʻO 80μL ka nui.
Koho 2.
ʻO QIAamp Viral RNA Mini Kit (52904) i hana ʻia e QIAGEN a i ʻole Nucleic Acid Extraction a i ʻole Purification Kit (YDP315-R) i hana ʻia e Tiangen Biotech (Beijing) Co., Ltd. ʻO ka nui o ka laʻana i unuhi ʻia he 140μL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 60μL.
