ʻO Adenovirus Universal
Inoa huahana
Pahu ʻIke Waikawa Nukleika Honua HWTS-RT017A Adenovirus (Fluorescence PCR)
ʻAlapia
No ka genus Mammalian adenovirus ka adenovirus kanaka (HAdV), he virus DNA kaula pālua me ka ʻole o ka envelope. ʻO nā adenovirus i loaʻa a hiki i kēia manawa he 7 mau subgroups (AG) a me 67 mau ʻano, nona he 55 serotypes he pathogenic i nā kānaka. I waena o lākou, ʻo ka mea hiki ke alakaʻi i nā maʻi ʻōpū hanu ʻo ia ka hui B (ʻAno 3, 7, 11, 14, 16, 21, 50, 55), Hui C (ʻAno 1, 2, 5, 6, 57) a me Hui E (ʻAno 4), a ʻo ka mea hiki ke alakaʻi i ka maʻi diarrhea ʻōpū ʻo ia ka Hui F (ʻAno 40 a me 41) [1-8]. Loaʻa i nā ʻano like ʻole nā hōʻailona lapaʻau like ʻole, akā ʻo ka mea nui nā maʻi ʻōpū hanu. ʻO nā maʻi hanu i hoʻokumu ʻia e nā maʻi ʻōpū hanu o ke kino kanaka no 5% ~ 15% o nā maʻi hanu honua, a me 5% -7% o nā maʻi hanu kamaliʻi honua [9]. He laha ka Adenovirus ma nā wahi like ʻole a hiki ke loaʻa i ka maʻi i ka makahiki holoʻokoʻa, ʻoi aku hoʻi ma nā wahi i piha i ka poʻe, kahi i maʻalahi i nā maʻi ahulau kūloko, ʻoiai ma nā kula a me nā kahua hoʻomoana koa.
Kanal
| ʻOhana | adenovirus ākeawaikawa nucleic |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | ʻUwi nasopharyngeal，ʻUhi ʻāʻī |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 Kope/mL |
| Kūlana kikoʻī | a) E hoʻāʻo i nā kuhikuhi maikaʻi ʻole o ka ʻoihana maʻamau e ka pahu hana, a hoʻokō ka hopena hoʻāʻo i nā koi. b) E hoʻohana i kēia pahu hana e ʻike ai a ʻaʻohe cross-reactivity me nā pathogens hanu ʻē aʻe (e like me ka maʻi Influenza A, ka maʻi Influenza B, ka maʻi Respiratory syncytial, ka maʻi Parainfluenza, Rhinovirus, Human metapneumovirus, etc.) a i ʻole bacteria (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, etc.). |
| Nā Mea Hana Pili | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli QuantStudio®5 Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.) ʻŌnaehana PCR Manawa Maoli LightCycler®480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plusnā (FQD-96A, Hangzhouʻenehana Bioer) MA-6000 Mea Hoʻopololei Wela Manawa Maoli (Suzhou Molarray Co., Ltd.) Nā ʻōnaehana PCR Manawa Maoli BioRad CFX96, nā ʻōnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
(1) Mea hoʻohuihui i manaʻo ʻia:ʻO ka Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Pono e hana ʻia ka unuhi ʻana e like me nā kuhikuhi. ʻO ka hāpana i unuhi ʻia nā mea maʻi'nā laʻana swab nasopharyngeal a i ʻole ka swab ʻāʻī i hōʻiliʻili ʻia ma kahi. E hoʻohui i nā laʻana i loko o ka reagent hoʻokuʻu laʻana e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex e hui maikaʻi, e kau ma ka mahana o ka lumi no 5 mau minuke, e lawe i waho a laila e hoʻohuli a hui maikaʻi e loaʻa ai ka DNA o kēlā me kēia laʻana.
(2) Mea hoʻohuihui i manaʻo ʻia:Hoʻāʻo Macro & Micro Hoʻolaha maʻi Pahu DNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).pono e hana ʻia ka hana e like me nā kuhikuhi. ʻO ka nui o ka laʻana i unuhi ʻia he 200µL, a me kaka nui o ka elution i ʻōlelo ʻiais80μL.
(3) Mea hoʻohui i manaʻo ʻia: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP)315) na Tiangen Biotech (Beijing) Co.,Ltd., kapono e hana ʻia ka hana e like me nā kuhikuhi. ʻO ka nui o ka laʻana i unuhi ʻia he 200µL, a me kaka nui o ka elution i ʻōlelo ʻiais80μL.
