ʻO ke Gene Kū'ē Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Inoa huahana
Pahu ʻIke ʻo HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) (Fluorescence PCR)
ʻAlapia
ʻO nā lāʻau antibiotic Carbapenem he mau lāʻau antibiotic β-lactam atypical me ka spectrum antibacterial ākea loa a me ka hana antibacterial ikaika loa. Ma muli o kona kūpaʻa i ka β-lactamase a me ka haʻahaʻa o ka toxicity, ua lilo ia i hoʻokahi o nā lāʻau antibacterial koʻikoʻi no ka mālama ʻana i nā maʻi bacteria koʻikoʻi. He kūpaʻa loa nā Carbapenems i nā plasmid-mediated extended-spectrum β-lactamases (ESBLs), chromosomes, a me nā plasmid-mediated cephalosporinases (AmpC enzymes).
Kanal
| PCR-Mix 1 | PCR-Mix 2 | |
| ʻOhana | IMP | VIM |
| VIC/HEX | Mana Kūloko | Mana Kūloko |
| CY5 | NDM | KPC |
| ROX | OXA48
| ʻOXA23 |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | ʻUha, kolone maʻemaʻe, ʻūpā rectal |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Kūlana kikoʻī | a) ʻIke ka pahu i nā kuhikuhi maikaʻi ʻole o ka ʻoihana maʻamau, a hoʻokō nā hopena i nā koi o nā kuhikuhi pili. b) Hōʻike nā hopena o ka hoʻāʻo cross-reactivity ʻaʻohe hopena cross o kēia pahu me nā pathogens hanu ʻē aʻe, e like me Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, a i ʻole nā laʻana i loaʻa nā genes pale lāʻau ʻē aʻe CTX, mecA, SME, SHV, TEM, etc. c) Kū'ē i ke komo ʻana: Ua koho ʻia ʻo Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin no ka hoʻāʻo komo ʻana, a hōʻike nā hopena ʻaʻohe hopena keakea o nā mea keakea i ʻōlelo ʻia ma luna nei i ka ʻike ʻana i nā genes kū'ē carbapenem ʻo KPC, NDM, OXA48, OXA23, VIM, a me IMP. |
| Nā Mea Hana Pili | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli QuantStudio®5 Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.) ʻŌnaehana PCR Manawa Maoli LightCycler®480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus (FQD-96A，Hangzhouʻenehana Bioer) MA-6000 Mea Hoʻopololei Wela Manawa Maoli (Suzhou Molarray Co., Ltd.) ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Koho 1.
Mea hoʻohuihui i manaʻo ʻia: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. E hoʻohui i 200μL o ka saline maʻamau i ka thallus precipitate. Pono nā ʻanuʻu ma hope e hahai i nā kuhikuhi no ka unuhi ʻana, a ʻo ka nui o ka elution i ʻōlelo ʻia100μL.
Koho 2.
ʻO ka mea hoʻohuihui i ʻōlelo ʻia: Nucleic Acid Extraction or Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd. Pono e hoʻomaka ka hoʻohuihui e like me ke kaʻina hana 2 o ke kuhikuhi no ka hoʻohana ʻana (e hoʻohui i 200μL o ka buffer GA i ka thallus precipitate, a lulu a hiki i ka wā e hoʻokuʻu loa ʻia ai nā thallus). E hoʻohana i ka wai manuahi RNase/DNase no ka elution, a ʻo ka nui o ka elution i ʻōlelo ʻia he 100μL.
Koho 3.
ʻO ka mea hoʻoheheʻe i ʻōlelo ʻia: Macro & Micro-Test Sample Release Reagent. Pono e holoi ʻia ka hāpana sputum ma ka hoʻohui ʻana i 1mL o ka saline maʻamau i ka thallus precipitate i mālama ʻia ma luna, centrifuged ma 13000r/min no 5 mau minuke, a hoʻolei ʻia ka supernatant (e mālama i 10-20µL o ka supernatant). No ka kolone maʻemaʻe a me ka swab rectal, e hoʻohui i 50μL o ka mea hoʻoheheʻe hāpana pololei i ka thallus precipitate i mālama ʻia ma luna, a pono e unuhi ʻia nā ʻanuʻu ma hope e like me ke kuhikuhi no ka hoʻohana ʻana.
