Kāleka Kālepaʻo Carbapenem
Hua waihona
HWTS-OT045 CarbapexM off (kpc / NDM / NDM / NDM 48 / VIM / Bluerescence PCR)
Epideminey
ʻO ka Carbapenem antibiotika he Ayypitinis β-Lactam antibiotics me keʻano antibacterial a me ka ikaika antibacterial. Ma muli o kona kūpaʻa i β-lactamase a me ka haʻahaʻa haʻahaʻa, ua lilo i mea nui o nā lāʻau antibacterial no ka mālamaʻana i nā maʻi nui. Ua paʻa loaʻo Carbapenems i ka plasmid-mediad i hoʻonuiʻia-cooctrum β-lactamoss (esbls)
Kauwe
PCR-Mix 1 | PCR-Mix 2 | |
Ka ha | Ip | Vim |
VIC / Hex | Kaohi kūloko | Kaohi kūloko |
Kūpono Kakau | Ndm | Kpc |
Rox | Paula48
| Paula23 |
Nā poʻoaʻenehana
Huanui | ≤-18 ℃ |
'Āmaʻomaʻo | 12 mau mahina |
ʻAno kiko'ī | ʻO Sputum, nā colinies maʻemaʻe, rectal swab |
Ct | ≤36 |
CV | ≤5.0% |
Lolulu | 103CFU / ML |
Kiko'ī | a) Uaʻike ka pahu i kaʻikepili maikaʻi loa i nā'ōlelo maikaʻiʻole, a hoʻokō nā hopena i nā koi o nā mea e pili ana i nāʻikepili. b)ʻO nā hopena o ka hōʻike hōʻike hōʻoia e hōʻike ana e hōʻike anaʻaʻole kēia i keʻano o ke kīleʻaleʻaleʻa, acadomellar Hanae nuemophilusanienzae, Acuetobacor Jukai, Aciaobicorie haemophiola, ka psenovitors he fliestant genx cenes cenx, Meca, sm, shv, shv, etc. c) Anti-interference: micin, micyclinem, clickomycin, callithromycin, cefrofloxacin i ka ike o Kāleka Carbapenem Reistsman gens kpc, NDM, Oxa48,ʻo Oxa23, VIM, a me ke ahiahi. |
Nā mea pili | Noi biosysteystems 7500 mau'ōnaehana PCR ʻO ka helu helu RETSTATIDO®5 i nā'ōnaehana PCR manawa SLAN-96P mea maoli-manawa PCR Systems (Hongshi lāʻau lapaʻauʻenehana co., Ltd.) ʻO Lightcycler®480 Reass-Time Systems PCR Laina linegene 9600 me ka'ōnaehana o PCR i ka manawaFqd -_a,Hangzhou'Techlelo Mīlani) Ma-6000 maoli-manawa nui i ka manawa nui Biorad CFX96 Nā Kūlana Kūlana Lacr Biorad CFX OPus 96 Reass PCR System |
Nā kahe o ka hana
Ke koho 1.
Hoʻonohonohoʻia ka uku houʻana: Macro & Micro-TRIC-miction General DNA / RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96. ka palaualelo thallus. Pono nā hana ma hope e hahai i nā'ōlelo no ka hoʻoiliʻana, a me ka leo i'ōleloʻia100μL.
Koho 2.
Hoʻonohonohoʻia ka uku houʻana:ʻO ka helu ACID ACID Active Excking a iʻole uku uku (YDD32) , E hoʻohana i ka wai RNAS / DNESE / DNESETER FREE NO EUTER, aʻo ka nui e hoʻoneʻeʻia ka nui o ka nui o ka EVOMME
Koho 3.
Hoʻonohonohoʻia ka uku uku Pono ka simple simple e holoiʻia e ka hoʻohuiʻana i ka 1ml o ka paʻakai maʻamau i nā minuke i hōʻikeʻia ma nā makahiki he 5000-. No ka colony bely