ʻŌnaehana Kūʻē Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Inoa mea kūʻai
HWTS-OT045 Carbapenem Kū'ē Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Kiko ʻIke (Fluorescence PCR)
Epidemiology
ʻO nā lāʻau lapaʻau Carbapenem he mau antibiotic β-lactam atypical me ka laulā antibacterial spectrum a me ka hana antibacterial ikaika loa.Ma muli o kona kūpaʻa i ka β-lactamase a me ka haʻahaʻa haʻahaʻa, ua lilo ia i kekahi o nā lāʻau antibacterial koʻikoʻi no ka mālama ʻana i nā maʻi bacterial koʻikoʻi.Paʻa loa nā Carbapenems i nā plasmid-mediated extended-spectrum β-lactamases (ESBLs), chromosomes, a me plasmid-mediated cephalosporinases (AmpC enzymes).
Kanal
| PCR-Mix 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Hoomalu Kuloko | Hoomalu Kuloko |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | ʻO ka sputum, nā kolone maʻemaʻe, ka swab rectal |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| kikoo | a) ʻIke ka kit i nā ʻōlelo hōʻike maikaʻi ʻole o ka hui, a ua kūpono nā hopena i nā koi o nā kuhikuhi pili. b) Hōʻike nā hopena o ka hoʻāʻo cross-reactivity ʻaʻohe pane kea o kēia kit me nā pathogens hanu ʻē aʻe, e like me Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenza. junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, a i ʻole nā laʻana i loaʻa nā genes kūʻē i ka lāʻau CTX, mecA, SME, SHV, TEM. c) Anti-interference: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin i koho ʻia no ka hoʻāʻo interference, a hōʻike nā hopena ʻaʻohe mea hoʻopilikia i nā mea i ʻōlelo ʻia ma luna. i ka ʻike ʻana i nā genes kūʻē carbapenem KPC, NDM, OXA48, OXA23, VIM, a me IMP. |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli QuantStudio®5 Pūnaehana PCR Manawa maoli SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd. ) LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli (FQD-96A，Hangzhouʻenehana bioer) MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
Manaʻo ʻia ka mea hoʻoheheʻe: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Hoʻohui i 200μL o ka saline maʻamau i ka thallus precipitate.Pono nā ʻanuʻu ma hope e hahai i nā ʻōlelo aʻoaʻo no ka unuhi ʻana, a ʻo ka nui o ka elution i ʻōlelo ʻia100μL.
Koho 2.
Manaʻo ʻia ka hoʻoneʻe ʻana: Nucleic Acid Extraction or Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd. Pono e hoʻomaka ka unuhi ʻana e like me ka pae 2 o ke aʻo ʻana no ka hoʻohana ʻana (e hoʻohui i 200μL o ka buffer GA i ka thallus precipitate , a lulu a hiki i ka pau ana o ka thallus).E hoʻohana i ka wai manuahi RNase/DNase no ka elution, a ʻo ka nui o ka elution i manaʻo ʻia he 100μL.
Koho 3.
Manaʻo ʻia ka mea hoʻoheheʻe: Macro & Micro-Test Sample Release Reagent.Pono e holoi ʻia ka laʻana sputum ma ka hoʻohui ʻana i 1mL o ka paʻakai maʻamau i ka thallus precipitate i ʻōlelo ʻia ma luna, centrifuged ma 13000r/min no 5 mau minuke, a hoʻolei ʻia ka supernatant (mālama i 10-20µL o supernatant).No ka colony maʻemaʻe a me ka rectal swab, hoʻohui i 50μL o ka laʻana hoʻokuʻu reagent pololei i ka thallus precipitate i ʻōlelo ʻia ma luna, a pono e unuhi ʻia nā ʻanuʻu e like me ke aʻo ʻana no ka hoʻohana ʻana.
