ʻO ka maʻi maʻi Dengue, Zika Virus a me Chikungunya Virus Multiplex
Inoa mea kūʻai
HWTS-FE040 Dengue Virus, Zika Virus a me Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi maʻi Dengue (DF), ka mea i hoʻoulu ʻia e ka maʻi dengue (DENV), ʻo ia kekahi o nā maʻi maʻi maʻi arbovirus.Loaʻa i kāna mea hoʻouna ʻo Aedes aegypti a me Aedes albopictus.Loaʻa ka DF ma nā wahi wela a me nā subtropical.No flavivirus ka DENV ma lalo o flaviviridae, a hiki ke hoʻokaʻawale ʻia i 4 serotypes e like me ka antigen surface.ʻO nā hōʻike maʻi o ka maʻi DENV ka nui o ka maʻi poʻo, ke kuni, ka nāwaliwali, ka hoʻonui ʻana o ka lymph node, ka leukopenia a me nā mea ʻē aʻe, a me ke koko, ka haʻalulu, ka ʻeha ʻeha a i ʻole ka make i nā hihia koʻikoʻi.I nā makahiki i hala iho nei, ʻo ka hoʻololi ʻana o ke aniau, ka hoʻonui ʻana i ke kūlanakauhale, ka hoʻomohala wikiwiki ʻana o ka mākaʻikaʻi a me nā mea ʻē aʻe i hāʻawi i nā kūlana wikiwiki a maʻalahi no ka hoʻouna ʻana a me ka hoʻolaha ʻana o DF, e alakaʻi ana i ka hoʻonui mau ʻana o ka maʻi maʻi o DF.
Kanal
| FAM | ʻakika nucleic MP |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | -18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | Serum hou |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kope/mL |
| kikoo | Hōʻike nā hopena hōʻike hoʻopilikia i ka wā ʻaʻole i ʻoi aku ka nui o ka bilirubin i loko o ka serum ma mua o 168.2μmol/ml, ʻaʻole ʻoi aku ka nui o ka hemoglobin i hana ʻia e ka hemolysis ma mua o 130g/L, ʻaʻole ʻoi aku ka nui o ka lipid koko ma mua o 65mmol/ml, ka huina IgG. ʻAʻole ʻoi aku ka nui o ka ʻike ma ka serum ma mua o 5mg/mL, ʻaʻohe hopena i ka maʻi dengue, Zika virus a i ʻole chikungunya virus detection.ʻO ka maʻi Hepatitis A, ka maʻi Hepatitis B, ka maʻi Hepatitis C, ka maʻi Herpes, ka maʻi hikina equine encephalitis, ka maʻi virus Hantavirus, ka maʻi Bunya, ka maʻi maʻi o West Nile a me nā hōʻailona serum genomic kanaka i kohoʻia no ka ho'āʻo cross-reactivity, a ua hōʻike nā hualoaʻaʻaʻohe ka hopena kea ma waena o kēia kit a me nā pathogens i ʻōlelo ʻia ma luna. |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR Manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
ʻO TIANamp Virus DNA/RNA Kit (YDP315-R), a pono e mālama ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana.ʻO 140μL ka nui o ka laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 60μL.
Koho 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., a pono e hana ʻia ka unuhi e like me ke aʻo ʻana no ka hoʻohana ʻana.ʻO 200μL ka nui o ka laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 80μL.
