ʻO ka maʻi maʻi Dengue, Zika Virus a me Chikungunya Virus Multiplex
inoa huahana
HWTS-FE040 Dengue Virus, Zika Virus a me Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi maʻi Dengue (DF), ka mea i hoʻoulu ʻia e ka maʻi dengue (DENV), ʻo ia kekahi o nā maʻi maʻi maʻi arbovirus. Loaʻa i kāna mea hoʻouna ʻo Aedes aegypti a me Aedes albopictus. Loaʻa ka DF ma nā wahi wela a me nā subtropical. No flavivirus ka DENV ma lalo o flaviviridae, a hiki ke hoʻokaʻawale ʻia i 4 serotypes e like me ka antigen surface. ʻO nā hōʻike maʻi o ka maʻi DENV ka nui o ka maʻi ʻeha, ke kuni, ka nāwaliwali, ka hoʻonui ʻana o ka puʻupuʻu lymph, ka leukopenia a me nā mea ʻē aʻe, a me ke koko, ka haʻalulu, ka ʻeha ʻeha a i ʻole ka make i nā hihia koʻikoʻi. I nā makahiki i hala iho nei, ua hāʻawi ka hoʻololi ʻana i ke aniau, ka hoʻonui ʻana i ke kūlanakauhale, ka hoʻomohala wikiwiki ʻana o ka mākaʻikaʻi a me nā mea ʻē aʻe i nā kūlana wikiwiki a kūpono hoʻi no ka hoʻouna ʻana a me ka hoʻolaha ʻana o DF, e alakaʻi ana i ka hoʻonui mau ʻana o ka maʻi maʻi o DF.
Kanal
| FAM | DENV nucleic acid |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | -18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | Serum hou |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | 500 kope/mL |
| kikoo | Hōʻike nā hopena hōʻike hoʻopilikia i ka wā ʻaʻole ʻoi aku ka nui o ka bilirubin i ka serum ma mua o 168.2μmol/ml, ʻaʻole i ʻoi aku ka nui o ka hemoglobin i hana ʻia e ka hemolysis ma mua o 130g/L, ʻaʻole ʻoi aku ka nui o ke koko lipid ma mua o 65mmol/ml, ʻaʻole ʻoi aku ka nui o ka IgG i loko o ka serum ma mua o 5mg/mL, ʻaʻohe hopena i ka maʻi maʻi dengue a i ʻole ka maʻi chikunguka. ʻO ka maʻi Hepatitis A, ka maʻi Hepatitis B, ka maʻi Hepatitis C, ka maʻi Herpes, ka maʻi maʻi hikina equine encephalitis, Hantavirus, ka maʻi Bunya, ka maʻi maʻi West Nile a me nā mea hoʻohālike genomic serum kanaka i koho ʻia no ka hoʻāʻo cross-reactivity, a ua hōʻike nā hopena ʻaʻohe ʻano ʻokoʻa ma waena o kēia pahu a me nā pathogens i ʻōlelo ʻia ma luna. |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
ʻO TIANamp Virus DNA/RNA Kit (YDP315-R), a pono e mālama ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO 140μL ka nui o ka laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 60μL.
Koho 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C) &00 ʻO Micro-Test Med-Tech Co., Ltd., a pono e hana ʻia ka ʻili e like me ke aʻo ʻana no ka hoʻohana. ʻO 200μL ka nui o ka laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 80μL.
