ʻO ka maʻi Dengue, ka maʻi Zika a me ka maʻi Chikungunya Multiplex
Inoa huahana
HWTS-FE040 Ka maʻi Dengue, ka maʻi Zika a me ka maʻi Chikungunya Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
ʻAlapia
ʻO ke kuni Dengue (DF), i hoʻoulu ʻia e ka maʻi dengue virus (DENV), ʻo ia kekahi o nā maʻi laha loa o ka arbovirus. ʻO kāna mea hoʻolaha e komo pū me Aedes aegypti lāua ʻo Aedes albopictus. ʻO ka DF ka mea nui ma nā wahi wela a me nā wahi subtropical. No ka flavivirus ma lalo o flaviviridae ʻo DENV, a hiki ke hoʻokaʻawale ʻia i 4 serotypes e like me ka antigen o ka ʻili. ʻO nā hōʻike lapaʻau o ka maʻi DENV e komo pū ana me ka ʻeha poʻo, ke kuni, ka nāwaliwali, ka hoʻonui ʻia ʻana o ka lymph node, leukopenia a me nā mea ʻē aʻe, a me ke kahe koko, ka haʻalulu, ka ʻeha ate a i ʻole ka make i nā hihia koʻikoʻi. I nā makahiki i hala iho nei, ua hāʻawi ka loli o ke aniau, ke kūlanakauhale, ka hoʻomohala wikiwiki ʻana o ka mākaʻikaʻi a me nā mea ʻē aʻe i nā kūlana wikiwiki a maʻalahi no ka hoʻouna ʻana a me ka hoʻolaha ʻana o DF, e alakaʻi ana i ka hoʻonui mau ʻana o ka wahi ahulau o DF.
Kanal
| ʻOhana | ʻAkika nukleika DENV |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | -18℃ |
| Ola papa | 9 mahina |
| ʻAno Laʻana | Serum hou |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | 500 Kope/mL |
| Kūlana kikoʻī | Hōʻike nā hopena hoʻāʻo hoʻopilikia i ka wā ʻaʻole i ʻoi aku ka nui o ka bilirubin i loko o ke koko ma mua o 168.2μmol/ml, ʻaʻole i ʻoi aku ka nui o ka hemoglobin i hana ʻia e ka hemolysis ma mua o 130g/L, ʻaʻole i ʻoi aku ka nui o ka lipid koko ma mua o 65mmol/ml, ʻaʻole i ʻoi aku ka nui o ka IgG i loko o ke koko ma mua o 5mg/mL, ʻaʻohe hopena i ka maʻi dengue, ka maʻi Zika a i ʻole ka ʻike ʻana i ka maʻi chikungunya. Ua koho ʻia nā maʻi Hepatitis A, ka maʻi Hepatitis B, ka maʻi Hepatitis C, ka maʻi Herpes, ka maʻi Eastern equine encephalitis, Hantavirus, ka maʻi Bunya, ka maʻi West Nile a me nā laʻana serum genomic kanaka no ka hoʻāʻo cross-reactivity, a hōʻike nā hopena ʻaʻohe hopena cross ma waena o kēia kit a me nā pathogens i ʻōlelo ʻia ma luna. |
| Nā Mea Hana Pili | ʻŌnaehana PCR Manawa Maoli Biosystems 7500 i hoʻopili ʻia Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli LightCycler®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Koho 1.
ʻO ka TIANamp Virus DNA/RNA Kit (YDP315-R), a pono e hana ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka laʻana i unuhi ʻia he 140μL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 60μL.
Koho 2.
ʻO ka Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., a pono e hana ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka laʻana i unuhi ʻia he 200μL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80μL.
