ʻO Enterovirus Universal
Inoa huahana
HWTS-EV001- Pahu ʻIke Waikawa Nuklea Honua o Enterovirus (Fluorescence PCR)
ʻAlapia
ʻO ka maʻi lima-wāwae-waha kahi maʻi lele i hoʻokumu ʻia e nā enteroviruses (EV). I kēia manawa, ua loaʻa he 108 mau ʻano serotypes o nā enteroviruses, i māhele ʻia i ʻehā mau pūʻulu: A, B, C a me D. I waena o lākou, ʻo ka enterovirus EV71 a me CoxA16 nā pathogens nui. Loaʻa pinepine ka maʻi i nā keiki ma lalo o 5 mau makahiki, a hiki ke hoʻoulu i ka herpes ma nā lima, nā wāwae, ka waha a me nā ʻāpana ʻē aʻe. E loaʻa i kahi helu liʻiliʻi o nā keiki nā pilikia e like me ka myocarditis, ka edema pulmonary, a me ka aseptic meningoencephalitis.
Kanal
| ʻOhana | RNA EV |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | ʻO ka ʻūpā oropharyngeal,Wai herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kope/mL |
| Nā Mea Hana Pili | Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500/7500, QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P LightCycler®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Koho 1.
Pahu Hoʻokaʻawale Manaʻo: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), pono e hoʻokaʻawale pono ʻia e like me nā kuhikuhi. ʻO ka nui o ka laʻana he 200 μL, ʻo ka nui o ka elution i manaʻo ʻia he 80µL.
Koho 2.
Pahu hoʻokaʻawale i ʻōlelo ʻia: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), pono e hoʻokaʻawale ʻia e like me nā kuhikuhi.
Koho 3.
Pahu hoʻokaʻawale i ʻōlelo ʻia: QIAamp Viral RNA Mini Kit (52904) a i ʻole Nucleic Acid Extraction a Purification Kit paha (YDP315-R), pono e hoʻokaʻawale ʻia e like me nā kuhikuhi. ʻO ka nui o ka laʻana he 140 μL, ʻo ka nui o ka elution i ʻōlelo ʻia he 60µL.
