Enterovirus Universal
Inoa mea kūʻai
HWTS-EV001- Enterovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi lima-wāwae-waha he maʻi lele ia e nā enteroviruses (EV).I kēia manawa, ua loaʻa nā ʻano serotypes o enteroviruses 108, i māhele ʻia i ʻehā mau pūʻulu: A, B, C a me D. I waena o lākou, enterovirus EV71 a me CoxA16 nā pathogens nui.Loaʻa ka maʻi i nā keiki ma lalo o 5 mau makahiki, a hiki ke hoʻoulu i ka herpes ma nā lima, nā wāwae, ka waha a me nā ʻāpana ʻē aʻe.E ulu ana kekahi helu liʻiliʻi o nā keiki i nā pilikia e like me ka myocarditis, ka edema pulmonary, a me ka maningoencephalitis aseptic.
Kanal
| FAM | EV RNA |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | Oropharyngeal swabʻO ka wai herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kope/mL |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500/7500 Fast Real-Time PCR Systems, QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
Manaʻo i ka pahu hopu: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor ( HWTS-3006B, HWTS-3006C), pono e unuhi ʻia e like me nā kuhikuhi.ʻO ka nui o ka laʻana he 200 μL, ʻo ka nui elution i ʻōlelo ʻia he 80μL.
Koho 2.
Manaʻo ʻia ka pahu hoʻoiho: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), pono e unuhi ʻia e like me nā kuhikuhi.
Koho3.
Paipai ʻia ka pahu pahu: QIAamp Viral RNA Mini Kit (52904) a i ʻole Nucleic Acid Extraction a Purification Kit (YDP315-R), pono e unuhi ʻia e like me nā kuhikuhi.ʻO ka nui o ka laʻana he 140 μL, ʻo ka nui elution i ʻōlelo ʻia he 60μL.
