Staphylococcus Aureus a me Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)

ʻO ka wehewehe pōkole:

Hoʻohana ʻia kēia pahu no ka ʻike qualitative o staphylococcus aureus a me nā methicillin-resistant staphylococcus aureus nucleic acids i loko o nā laʻana sputum kanaka, nasal swab samples a me ka ʻili a me nā mea maʻi palupalu palupalu i loko o vitro.


Huahana Huahana

Huahana Huahana

Inoa mea kūʻai

HWTS-OT062 Staphylococcus Aureus a me Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit(Fluorescence PCR)

Palapala

CE

Epidemiology

ʻO Staphylococcus aureus kekahi o nā maʻi pathogenic koʻikoʻi o ka maʻi nosocomial.No ka staphylococcus 'o Staphylococcus aureus (SA) a he 'elele ia no ka ma'i bacteria Gram-positive, hiki ke ho'opuka i nā 'ano mea 'awa'awa a me nā enzyme invasive.Loaʻa i ka bacteria nā hiʻohiʻona o ka hoʻolaha ākea, ka pathogenicity ikaika a me ka nui o ke kū'ē kiʻekiʻe.ʻO ke ʻano nuclease Thermotable (nuc) kahi gene i mālama nui ʻia o staphylococcus aureus.

Kanal

FAM gene mecA kū'ē i ka methicillin
ROX

Hoomalu Kuloko

CY5 staphylococcus aureus nuc gene

Nā ʻāpana ʻenehana

Waihona ≤-18 ℃ & pale ʻia mai ka mālamalama
Kau-ola 12 mahina
ʻAno Hoʻohālike sputum, ʻili a me ka ʻiʻo palupalu nā mea maʻi, a me nā laʻana nasal swab
Ct ≤36
CV ≤5.0%
LoD 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL bacteria kū'ē i ka methicillin.Ke ʻike ka pahu i ka ʻōlelo LoD aupuni, hiki ke ʻike ʻia ka 1000/mL staphylococcus aureus.
kikoo Hōʻike ka hoʻāʻo cross-reactivity ʻaʻohe o kēia kit me nā mea maʻi hanu ʻē aʻe e like me ka methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiumaniella protein. mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae.
Nā mea hana pili Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli

QuantStudio®5 Pūnaehana PCR manawa maoli

SLAN-96P Pūnaehana PCR Manawa maoli

LightCycler®480 Pūnaehana PCR manawa maoli

LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli

MA-6000 ʻOiaʻiʻo ka nui o ka wela wela

BioRad CFX96 Pūnaehana PCR Manawa maoli

BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli

Holo Hana

Koho 1.

Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. hiki ke hoʻohana me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).E hoʻohui i 200µL o ka paʻakai maʻamau i ka precipitate i hana ʻia, a pono e unuhi ʻia nā ʻanuʻu ma hope e like me nā kuhikuhi, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80µL.

Koho 2.

ʻO Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E hoʻohui i 1mL o ka paʻakai maʻamau i ka precipitate ma hope o ka holoi ʻana me ka saline maʻamau, a laila hui maikaʻi.ʻO Centrifuge ma 13,000r/min no 5 mau minuke, e wehe i ka supernatant (mālama ʻia 10-20µL o supernatant), a hahai i nā kuhikuhi no ka unuhi ʻana ma hope.

Manaʻo ʻia ka mea hoʻoheheʻe ʻoki: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd.Pono e hoʻokō pono ʻia ka unuhi ʻana e like me ka ʻanuʻu 2 o ka papa kuhikuhi.Manaʻo ʻia e hoʻohana i ka wai RNase a me DNase-free no ka elution me ka nui o 100µL.

 


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