Zika Virus

ʻO ka wehewehe pōkole:

Hoʻohana ʻia kēia pahu e ʻike pono i ka Zika virus nucleic acid i loko o nā laʻana serum o nā maʻi i manaʻo ʻia he maʻi Zika virus in vitro.


Huahana Huahana

Huahana Huahana

Inoa mea kūʻai

HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)

Palapala

CE

Epidemiology

No ka genus Flaviviridae ka ma'i Zika, he ma'i ma'i RNA i ho'opa'a 'ia me ka anawaena o 40-70nm.He envelop kona, aia 10794 nucleotides, a hoʻopili i 3419 amino acids.Wahi a ka genotype, ua māhele ʻia i ke ʻano ʻApelika a me ke ʻano ʻAsia.ʻO ka maʻi maʻi Zika kahi maʻi maʻi maʻi e hoʻemi ʻia e ka maʻi maʻi Zika, ka mea i lawe nui ʻia ma o ka nahu ʻana o nā makika Aedes aegypti.ʻO nā hiʻohiʻona o ka maʻi he maʻi kuni, ʻūlū, arthralgia a i ʻole conjunctivitis, a ʻaʻohe mea make.Wahi a ka World Health Organization, hiki ke pili i ka microcephaly neonatal a me Guillain-Barre syndrome (Guillain-Barré syndrome) me ka maʻi maʻi Zika.

Kanal

FAM Zika virus nucleic acid
ROX

Hoomalu Kuloko

Nā ʻāpana ʻenehana

Waihona ≤30 ℃ & pale ʻia mai ka mālamalama
Kau-ola 12 mahina
ʻAno Hoʻohālike serum hou
Ct ≤38
CV ≤5.0%
LoD 500 ng/μL
kikoo ʻAʻole pili nā hopena hoʻāʻo i loaʻa i kēia pahu e ka hemoglobin (<800g/L), bilirubin (<700μmol/L), a me nā lipids koko/triglycerides (<7mmol/L) i ke koko.
Nā mea hana pili ABI 7500 Pūnaehana PCR manawa maoli

ʻO ABI 7500 ʻO nā Pūnaehana PCR ʻOiaʻiʻo wikiwiki

QuantStudio®5 Pūnaehana PCR manawa maoli

SLAN-96P Pūnaehana PCR Manawa maoli

LightCycler®480 Pūnaehana PCR manawa maoli

LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli

MA-6000 ʻOiaʻiʻo ka nui o ka wela wela

ʻO BioRad CFX96 Pūnaehana PCR manawa maoli, BioRad CFX Opus 96 Pūnaehana PCR manawa maoli.

Holo Hana

Koho 1.

QIAamp Viral RNA Mini Kit(52904), Nucleic Acid Extraction a i ʻole Purification Reagent(YDP315-R) na Tiangen Biotech(Beijing) Co.,Ltd.ʻO ka unuhiPono e unuhi ʻia e like me nā ʻōlelo aʻoaʻo, a ʻo ka nui o ka unuhi ʻōlelo ʻia he 140 μL a ʻo ka nui elution i ʻōlelo ʻia he 60 μL.

Koho 2.

Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Pono e unuhiʻia ka unuhiʻana e like me nā kuhikuhi.ʻO 200 μL ka nui o ka laʻana, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80μL.


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