ʻO Zaire a me Sudan Ebolavirus Nucleic Acid i hoʻomaloʻo ʻia
inoa huahana
HWTS-FE035-Hoʻomaloʻo ʻia ʻo Zaire a me Sudan Ebolavirus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
No Filoviridae ka Ebolavirus, he ma'i ma'i ma'i RNA i ho'oka'awale 'ole. ʻO nā maʻi virus he mau filament lōʻihi me ka awelika virion lōʻihi o 1000nm a me ke anawaena o kahi 100nm. ʻO ka genome Ebolavirus he RNA ʻino-ʻole i hoʻokaʻawale ʻia me ka nui o 18.9kb, e hoʻopili ana i 7 mau protein kūkulu ʻia a me 1 protein non-structural. Hiki ke hoʻokaʻawale ʻia ka Ebolavirus i nā ʻano like me Zaire, Sudan, Bundibugyo, Tai Forest a me Reston. Ma waena o lākou, ua hōʻike ʻia ke ʻano Zaire a me ke ʻano Sudan i mea e make ai ka poʻe he nui mai ka maʻi. ʻO EHF (Ebola Hemorrhagic Fever) he maʻi maʻi maʻi hemorrhagic i hoʻokumu ʻia e ka Ebolavirus. Hoʻopili nui ʻia nā kānaka ma ka hoʻopili ʻana me nā wai kino, nā mea huna a me nā excreta o nā mea maʻi a i ʻole nā holoholona maʻi, a ʻo nā hōʻike lapaʻau ka nui o ke kuni, ke koko a me nā pōʻino kino he nui. Loaʻa i ka EHF ka nui o ka make nui o 50%-90%. I kēia manawa, ʻo nā ʻano o ka maʻi Ebolavirus ka nui o nā hoʻokolohua lab, me nā ʻano ʻelua: etiological detection a me serological detection. ʻO ka ʻike etiological e pili ana i ka ʻike ʻana i nā antigen viral i loko o nā laʻana koko e ELISA, ka ʻike ʻana i nā waikawa nucleic ma nā ʻano hoʻonui e like me RT-PCR, etc., a me ka hoʻohana ʻana i nā cell Vero, Hela, etc. Loaʻa ka ʻike serological i ka ʻike ʻana i nā antibodies IgM kikoʻī Serum ma ka hopu ʻana iā ELISA, a me ka ʻike ʻana i nā antibodies IgG kikoʻī Serum e ELISA, immunofluorescence, etc.
Nā ʻāpana ʻenehana
| Waihona | ≤30 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | serum,plasma laʻana |
| CV | ≤5.0% |
| LoD | 500 kope/μL |
| Nā mea hana pili | Hoʻohana ʻia i ka ʻano I detection reagent: Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli, QuantStudio®5 Pūnaehana PCR manawa maoli, SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), ʻO BioRad CFX96 Pūnaehana PCR manawa maoli, BioRad CFX Opus 96 Pūnaehana PCR manawa maoli. E pili ana i ke ʻano II detection reagent: EudemonaTMAIO800 (HWTS-EQ007) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Holo Hana
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Pono e hana ʻia ka unuhi e like me nā ʻōlelo aʻoaʻo, a ʻo ka nui o ka laʻana i unuhi ʻia he 200μL a ʻo ka nui elution i ʻōlelo ʻia he 80μL.
