Hepatitis B Virus RNA
Inoa mea kūʻai
HWTS-HP007 Hepatitis B Virus RNA Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
QʻO ka ʻike ʻana i nā pae HBV-RNA i loko o ka serum hiki ke nānā maikaʻi i ke kiʻekiʻe o ka HBV cccDNA i loko o nā hepatocytes, a i ka manawa like e pale aku ai i ka hōʻeha nui ʻana i nā maʻi.He koʻikoʻi nui ia no ka ʻike kōkua o ka maʻi hepatitis B (HBV) a me ka nānā ʻana i ka pono a me ka wehe ʻana i ka lāʻau lapaʻau o NAs antiviral therapy i nā maʻi CHB.
Kanal
| FAM | HBV-RNA |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | serum |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 100 kope/mL |
| kikoo | Hōʻike nā hopena hoʻokolohua interference ʻaʻohe hopena i ka hoʻoholo ʻana o ka HBV RNA i ka wā ʻaʻole ʻoi aku ka nui o ka bilirubin i loko o ka serum ma mua o 168.2μmol/mL, ʻaʻole i ʻoi aku ka nui o ka hemoglobin i hana ʻia e ka hemolysis ma mua o 130g/L, ʻAʻole iʻoi aku ka nui o nā lipids ma mua o 65mmol / mL;ʻAʻohe hopena i ka hoʻoholo ʻana o ka HBV RNA inā ʻaʻole ʻoi aku ka nui o ka IgG i loko o ka serum ma mua o 5mg/mL.Hōʻike nā hopena hoʻāʻo cross-reactivity ʻaʻohe ʻano hoʻololi ma waena o kēia kit a me nā maʻi ʻē aʻe a i ʻole ka bacteria i ʻike ʻia e ka nucleic acid i loko o nā laʻana koko (virus hepatitis C, cytomegalovirus kanaka, virus epstein-barr, virus immunodeficiency kanaka, maʻi hepatitis A, syphilis. , ka ma'i ma'i herpes kanaka type 6, ka ma'i ma'i herpes simplex type 1, ka ma'i ma'i herpes simplex type 2, ka ma'i influenza A, propionibacterium acnes, staphylococcus aureus, candida albicans) a me na genomes kanaka;Ma hope o ka lawe ʻana o nā mea maʻi i ka lamivudine, telbivudine, adefovir dipivoxil a me entecavir, i ka wā i ʻoi aku ka nui o ka viral load o ka maʻi hepatitis B i ke kino ma luna o ka LoD liʻiliʻi o ka pahu, hiki ke ʻike pono ʻia ka pahu. |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoliHoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.) |
Holo Hana
Koho 1.
ʻO Viral DNA/RNA Isolation Kit (NA007-2) na GenMag Biotechnology Co., Ltd. E ʻoluʻolu e hahai i nā ʻōkuhi kikoʻī kikoʻī i ka palapala aʻo.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hoʻokō ʻia ka unuhi e like me ka manual instruction, ʻo 200μL ka nui o ka hāpana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia. 80μL.
