Herpes Simplex Virus Type 1/2,(HSV1/2) Nucleic Acid
Inoa mea kūʻai
HWTS-UR018A-Herpes simplex mea hoʻomaʻi ʻano 1/2, (HSV1/2) pahu ʻike ʻakika nucleic (Fluorescence PCR)
Epidemiology
ʻO nā maʻi i hoʻouna ʻia i ke kāne (STD) kekahi o nā mea hoʻoweliweli nui i ka palekana olakino o ka honua.ʻO ia mau maʻi hiki ke alakaʻi i ka hānau keiki ʻole, ka hānau ʻana o ka fetal premature, ka ʻōpū a me nā pilikia koʻikoʻi like ʻole.Nui nā ʻano maʻi maʻi STD, ʻo ia hoʻi nā bacteria, viruses, chlamydia, mycoplasma a me spirochetes, ma waena o nā Neisseria gonorrhoeae, Mycoplasma genitalium, Chlamydia trachomatis, HSV1, HSV2, Mycoplasma hominis, a me Ureaplasma urealyticum ka mea maʻamau.
ʻO ka maʻi herpes genital kahi maʻi maʻamau i lawe ʻia e ka HSV2, kahi maʻi maʻi loa.I nā makahiki i hala iho nei, ua piʻi nui ka maʻi o ka herpes genital, a ma muli o ka piʻi ʻana o nā ʻano moe kolohe, ua piʻi ka nui o ka ʻike ʻana no ka HSV1 i ka herpes genital a ua hōʻike ʻia he kiʻekiʻe e like me 20% -30%.ʻO ka maʻi mua me ka maʻi herpes genital ka hapa nui me ka ʻole o nā hōʻailona lapaʻau ʻike ʻia koe wale nō ka herpes kūloko i ka mucosa a i ʻole ka ʻili o kekahi mau maʻi.Ma muli o ka hōʻike ʻana o ka herpes genital i ka hoʻokahe ʻana i ke ola a me ka hoʻi hou ʻana, he mea nui ia e nānā i nā pathogens i ka wā hiki loa a hoʻopaʻa i kāna hoʻouna ʻana.
Kanal
FAM | HSV1 |
CY5 | HSV2 |
VIC(HEX) | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
Waihona | Ka wai: ≤-18 ℃ I ka pōʻeleʻele |
Kau-ola | 12 mahina |
ʻAno Hoʻohālike | urethral secretions, huna huna |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 Kope / pane |
kikoo | ʻAʻohe hoʻololi keʻa me nā mea maʻi STD ʻē aʻe e like me Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, a me Ureaplasma urealyticum. |
Nā mea hana pili | Hiki iā ia ke hoʻohālikelike i nā mea kani PCR fluorescent mainstream ma ka mākeke. Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Manaʻo ʻia ka mea hoʻoheheʻe: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).Pono e hoʻokō pono ʻia ka unuhi ʻana e like me nā kuhikuhi.
Manaʻo ʻia ka hoʻoulu ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test ʻO ka mea hoʻoheheʻe Nucleic Acid Automatic (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e mālama pono ʻia ka unuhi e like me nā kuhikuhi.ʻO ka nui o ka elution i manaʻo ʻia he 80 μL.
Manaʻo ʻia ka mea hoʻoneʻe ʻoki: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315) na Tiangen Biotech(Beijing) Co., Ltd.Pono e hoʻokō pono ʻia ka unuhi ʻana e like me nā kuhikuhi.ʻO ka nui o ka elution i manaʻo ʻia he 80 μL.