Kanaka Braf Gene Gene v600e Muttation
Hua waihona
Hwts-TM007-Human Braf Gene Gury Gene V600E MUTERE EMINA ORTORS ENT (Fluorscence PCR)
HAAKAANA
Ce / tfda
Epideminey
ʻOi aku ka loaʻa o nā mea he 30 ma mua o nāʻano o nāʻano braf braf, kahi e pili ana i ka 90% i loaʻa ma ka manawa 15 adentine (a), hopena i ka hoʻololiʻana o ka valine (v) ma ke kūlana 600 e glutamic acid (e) i ka huahana protein. ʻIke pinepineʻia nā hui Braf ma nā'āpana helgnant e like me ka melanoma, colcer cancer, a me ka maʻi kanesar. ʻO ka hoʻomaopopoʻana i ka hāʻawiʻana o keʻano o Braf Gene i lilo i mea pono e pale ai i nā lāʻau lapaʻau i hoʻopaʻaʻia i ka lāʻau lapaʻau i hiki ke loaʻa.
Kauwe
Ka ha | V600E MUTation, ka mana kūloko |
Nā poʻoaʻenehana
Huanui | ≤-18 ℃ |
'Āmaʻomaʻo | 9 mau mahina |
ʻAno kiko'ī | paraffin-improdded pathological patister |
CV | <5.0% |
Ct | ≤38 |
Lolulu | E hoʻohana i nā kī eʻike ai i ka mana kūpono o ka pā. a) Ma lalo o 3ng / μl wild-type-type-typed, 1% helu helu i hiki keʻikeʻia ma keʻano o ka hopena b) ma lalo o 1% helu helu helu, ka heluʻana o ka 1 × 103Copies / ml i ka hihiu hihiu o 1 × 105Hiki i nā kope / ML keʻikeʻia i loko o keʻano o ka waiwai; c) hiki i ka mea ke nānā aku i keʻano o keʻano o keʻano o keʻano he haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa sw i ka mana o kaʻoihana kūloko. |
Nā mea pili: | Noi biosysteystems 7500 mau'ōnaehana PCRHoʻopili i ka biosystems 7300 maoli o PCR Nā'ōnaehana,ʻo ka helu helu helu 5 mau manawa hana maoli ʻO Lightcycler®480 Reass-Time Systems PCR Biorad CFX96 Nā Kūlana Kūlana Lacr |
Nā kahe o ka hana
Hoʻonohonohoʻia nā uku uku: Qiagen's Qiamp DNA FFPE Tissie TISSUS (DIAPECE-ENJEVED ACSUND BILES (DIPPING) CO., LTD.