Ka Hoʻololi ʻana o ka Gene BRAF Kanaka V600E
Inoa huahana
HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit (Fluorescence PCR)
Palapala hōʻoia
CE/TFDA
ʻAlapia
Ua ʻike ʻia ma mua o 30 mau ʻano o nā hoʻololi BRAF, ma kahi o 90% o ia mau mea aia ma ka exon 15, kahi i manaʻo ʻia ai ka hoʻololi ʻana o V600E ʻo ia ka hoʻololi maʻamau, ʻo ia hoʻi, ua hoʻololi ʻia ka thymine(T) ma ke kūlana 1799 ma ka exon 15 i adenine (A), e hopena ana i ka hoʻololi ʻana o ka valine (V) ma ke kūlana 600 e ka glutamic acid (E) i loko o ka huahana protein. Loaʻa pinepine nā hoʻololi BRAF i nā ʻōpū ʻino e like me ka melanoma, ke kanesa colorectal, ke kanesa thyroid, a me ke kanesa māmā. ʻO ka hoʻomaopopo ʻana i ka hoʻololi ʻana o ka gene BRAF ua lilo ia i mea e pono ai ke nānā i nā lāʻau EGFR-TKI a me nā lāʻau lapaʻau i hoʻopili ʻia i ka hoʻololi ʻana o ka gene BRAF i ka lāʻau lapaʻau i hoʻopili ʻia no nā maʻi e hiki ke pōmaikaʻi.
Kanal
| ʻOhana | Hoʻololi V600E, ka mana kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 9 mahina |
| ʻAno Laʻana | nā laʻana ʻiʻo pathological i hoʻokomo ʻia i ka paraffin |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | E hoʻohana i nā kime e ʻike i ka mana maikaʻi LoD e pili ana. a) ma lalo o 3ng/μL wild-type background, hiki ke ʻike ʻia ka 1% mutation rate i loko o ka reaction buffer me ke kūpaʻa; b) ma lalo o 1% mutation rate, ka mutation o 1 × 103Nā kope/mL ma ke kua ʻano hihiu o 1 × 105Hiki ke ʻike paʻa ʻia nā kope/mL i loko o ka buffer reaction; c) hiki i ka IC Reaction Buffer ke ʻike i ka palena ʻike haʻahaʻa loa o ka mana hoʻomalu maikaʻi SW3 o ka mana kūloko o ka hui. |
| Nā Mea Hana Pili: | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa MaoliʻO ka Biosystems i hoʻopili ʻia 7300 PCR Manawa Maoli Nā ʻōnaehana, QuantStudio® 5 Nā ʻōnaehana PCR Manawa Maoli ʻŌnaehana PCR Manawa Maoli LightCycler®480 ʻŌnaehana PCR Manawa Maoli BioRad CFX96 |
Kahe Hana
Nā mea hoʻohuihui i ʻōlelo ʻia: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) i hana ʻia e Tiangen Biotech (Beijing) Co., Ltd.
