Kanaka EGFR Gene 29 Mutations
Inoa mea kūʻai
HWTS-TM001A-Human EGFR Gene 29 Mutations Detection Kit (Fluorescence PCR)
Epidemiology
Ua lilo ka ma'i 'a'ai 'o ke kumu nui o ka make 'ana o ka ma'i 'a'ai ma ka honua holo'oko'a, e ho'oweliweli nui ana i ke ola kanaka.ʻO ka maʻi maʻi ʻaʻai liʻiliʻi liʻiliʻi ma kahi o 80% o nā maʻi maʻi maʻi maʻi.ʻO EGFR i kēia manawa ka pahuhopu molekala nui loa no ka mālama ʻana i ka maʻi maʻi ʻaʻai liʻiliʻi liʻiliʻi.Hiki i ka phosphorylation o EGFR ke hoʻoikaika i ka ulu ʻana o ka cell tumor, ka ʻokoʻa, ka hoʻouka ʻana, ka metastasis, ka anti-apoptosis, a me ka hoʻoikaika ʻana i ka angiogenesis tumor.Hiki i ka EGFR tyrosine kinase inhibitors (TKI) ke ālai i ke ala hōʻailona EGFR ma ke kāohi ʻana i ka autophosphorylation EGFR, ma laila e pale ai i ka hoʻonui a me ka hoʻokaʻawale ʻana o nā cell tumor, e hāpai ana i ka apoptosis cell tumor, e hōʻemi ana i ka angiogenesis tumor, etc.Ua hōʻike ʻia kahi helu nui o nā haʻawina e pili pono ana ka therapeutic efficacy o EGFR-TKI i ke kūlana o ka mutation gene EGFR, a hiki ke pale pono i ka ulu ʻana o nā pūnaewele tumora me ka mutation gene EGFR.Aia ka gene EGFR ma ka lima pōkole o ka chromosome 7 (7p12), me ka lōʻihi piha o 200Kb a he 28 exons.Aia ka ʻāina mutated i nā exons 18 a i 21, codons 746 a 753 hoʻopau ʻana i ka mutation ma exon 19 mau helu no kahi 45% a me ka L858R mutation ma exon 21 mau helu no kahi 40% a 45%.ʻO ka NCCN Guidelines for the Diagnosis and Treatment of Non-Small Cell Lung Cancer e hōʻike maopopo ana he pono ka hoʻāʻo ʻana o ka gene mutation EGFR ma mua o ka hoʻokele EGFR-TKI.Hoʻohana ʻia kēia pahu hoʻāʻo e alakaʻi i ka hoʻokele ʻana i nā lāʻau epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), a hāʻawi i ke kumu no ka lāʻau lapaʻau pilikino no nā poʻe maʻi me ka maʻi maʻi ʻaʻa ʻole liʻiliʻi.Hoʻohana wale ʻia kēia pahu no ka ʻike ʻana i nā hoʻololi maʻamau i ka gene EGFR i nā poʻe maʻi me ka maʻi maʻi ʻaʻai liʻiliʻi liʻiliʻi.ʻO nā hopena hōʻike no ka hōʻike lapaʻau wale nō a ʻaʻole pono e hoʻohana ʻia ma ke kumu hoʻokahi no ka mālama ʻana i nā maʻi.Pono nā kauka lapaʻau e noʻonoʻo i ke kūlana o ka mea maʻi, nā hōʻailona lāʻau, a me ka mālama ʻana.
Kanal
| FAM | Hoʻopaʻa IC, L858R Reaction Buffer, 19del Reaction Buffer, T790M Reaction Buffer, G719X Reaction Buffer, 3Ins20 Reaction Buffer, L861Q Reaction Buffer, S768I Reaction Buffer |
Nā ʻāpana ʻenehana
| Waihona | Ka wai: ≤-18 ℃ I ka pōʻeleʻele;Lyophilized: ≤30 ℃ I ka pōʻeleʻele |
| Kau-ola | Ka wai: 9 mahina;Lyophilized: 12 mahina |
| ʻAno Hoʻohālike | ʻiʻo maʻi maʻi hou, ʻāpana pathological maloʻo, ʻiʻo pathological i hoʻokomo ʻia i ka paraffin a ʻāpana paha, plasma a i ʻole serum |
| CV | <5.0% |
| LoD | ka ʻike ʻana i ka hopena hopena nucleic acid ma lalo o ke kāʻei o 3ng/μL wild-type, hiki ke ʻike paʻa i ka 1% mutation rate |
| kikoo | ʻAʻohe hana keʻa me ka DNA genomic kanaka ʻano hihiu a me nā ʻano mutant ʻē aʻe |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoliHoʻohana ʻia Biosystems 7300 Pūnaehana PCR manawa maoli QuantStudio® 5 Pūnaehana PCR Manawa maoli LightCycler® 480 Pūnaehana PCR manawa maoli BioRad CFX96 Pūnaehana PCR Manawa maoli |
Holo Hana
