ʻAkika Nucleic B27 Leukocyte kanaka
Inoa huahana
HWTS-GE011A-Human Leukocyte Antigen B27 Nucleic Acid Detection Kit (Fluorescence PCR)
ʻAlapia
ʻO ka Ankylosing spondylitis (AS) kahi maʻi mumū holomua e hoʻouka nui ana i ka iwi kuamoʻo a hiki ke hoʻopili i nā hono sacroiliac a me nā hono a puni i nā kekelē like ʻole. Ua hōʻike ʻia ua hōʻike ʻo AS i ka hōʻuluʻulu ʻohana maopopo a pili loa ia i ka antigen leukocyte kanaka HLA-B27. I loko o ke kanaka, ua ʻike ʻia a ʻike ʻia ma mua o 70 mau ʻano o nā ʻano HLA-B27, a ʻo ia hoʻi, ʻo HLA-B*2702, HLA-B*2704 a me HLA-B*2705 nā ʻano maʻamau e pili ana i ka maʻi. Ma Kina, Singapore, Iapana a me ka ʻāpana ʻo Taiwan o Kina, ʻo ka ʻano maʻamau o HLA-B27 ʻo HLA-B*2704, e helu ana ma kahi o 54%, ukali ʻia e HLA-B*2705, e helu ana ma kahi o 41%. Hiki i kēia pahu hana ke ʻike i ka DNA ma nā ʻano like ʻole HLA-B*2702, HLA-B*2704 a me HLA-B*2705, akā ʻaʻole ia e hoʻokaʻawale iā lākou mai kekahi i kekahi.
Kanal
| ʻOhana | HLA-B27 |
| VIC/HEX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | Wai: ≤-18 ℃ I ka pōʻeleʻele |
| Ola papa | Wai: 18 mahina |
| ʻAno Laʻana | nā hāpana koko holoʻokoʻa |
| Ct | ≤40 |
| CV | ≤5.0% |
| LoD | 1ng/μL |
| Kūlana kikoʻī
| ʻAʻole e hoʻopilikia ʻia nā hopena hoʻāʻo i loaʻa e kēia pahu hana e ka hemoglobin (<800g/L), bilirubin (<700μmol/L), a me nā lipids koko/triglycerides (<7mmol/L) i loko o ke koko. |
| Nā Mea Hana Pili | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana Biosystem i hoʻopili ʻia ʻo StepOne Real-Time PCR Systems ʻŌnaehana PCR Manawa Maoli LightCycler®480 ʻŌnaehana Q-PCR Agilent-Stratagene Mx3000P |
