Kanaka Leukocyte Antigen B27 Nucleic Acid
Inoa mea kūʻai
HWTS-GE011A-Human Leukocyte Antigen B27 Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
ʻO Ankylosing spondylitis (AS) kahi maʻi ʻehaʻeha holomua e hoʻopiʻi nui ʻia i ka spine a hiki ke komo i nā hui sacroiliac a me nā hono a puni i nā pae like ʻole.Ua hōʻike ʻia e hōʻike ana ʻo AS i ka hōʻuluʻulu ʻohana maopopo a pili loa i ka antigen leukocyte kanaka HLA-B27.I loko o ke kanaka, ʻoi aku ma mua o 70 mau ʻano o nā ʻano subtypes HLA-B27 i ʻike ʻia a ʻike ʻia, a ʻo lākou, HLA-B*2702, HLA-B*2704 a me HLA-B*2705 nā ʻano subtypes maʻamau e pili ana i ka maʻi.Ma Kina, Singapore, Iapana a me Taiwan ka moku o Kina, ʻo ka subtype maʻamau o HLA-B27 ʻo HLA-B*2704, helu ʻia ma kahi o 54%, a ukali ʻia e HLA-B*2705, ʻo ia ka helu ma kahi o 41%.Hiki i kēia pahu ke ʻike i ka DNA ma nā ʻano subtypes HLA-B*2702, HLA-B*2704 a me HLA-B*2705, akā ʻaʻole ia e hoʻokaʻawale iā lākou mai kekahi i kekahi.
Kanal
| FAM | HLA-B27 |
| VIC/HEX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | Ka wai: ≤-18 ℃ I ka pōʻeleʻele |
| Kau-ola | Ka wai: 18 mahina |
| ʻAno Hoʻohālike | nā laʻana koko holoʻokoʻa |
| Ct | ≤40 |
| CV | ≤5.0% |
| LoD | 1ng/μL |
| kikoo
| ʻAʻole pili nā hopena hoʻāʻo i loaʻa i kēia pahu e ka hemoglobin (<800g/L), bilirubin (<700μmol/L), a me nā lipids koko/triglycerides (<7mmol/L) i ke koko. |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystem StepOne Pūnaehana PCR manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli Pūnaehana Q-PCR Agilent-Stratagene Mx3000P |
