Influenza A Virus/ Influenza B Virus
inoa huahana
HWTS-RT174-Influenza A Virus/ Influenza B Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ma muli o nā ʻokoʻa antigenic ma waena o NP gene a me M gene, hiki ke hoʻokaʻawale ʻia nā maʻi maʻi influenza i ʻehā mau ʻano: ka maʻi maʻi influenza A (IFV A), ka maʻi maʻi influenza B (IFV B), ka maʻi influenza C (IFV C) a me ka maʻi influenza D (IFV D)[1]. ʻO ka maʻi maʻi Influenza A he nui nā pūʻali a me nā serotypes paʻakikī, a hiki ke loaʻa ka hiki ke hoʻolaha ma waena o nā pūʻali ma o ka hoʻohui hou ʻana a me ka hoʻololi ʻana. Loaʻa i nā kānaka ka palekana mau loa i ka maʻi maʻi influenza A, no laila hiki ke maʻalahi ka poʻe o nā makahiki āpau. ʻO ka maʻi maʻi Influenza A ke kumu nui o ka maʻi maʻi influenza[2]. Loaʻa ka maʻi maʻi Influenza B ma kahi liʻiliʻi a ʻaʻohe ʻano subtypes i kēia manawa. ʻO nā mea nui e hoʻoulu ai i ka maʻi o ke kanaka ʻo ka laina B/Yamagata a i ʻole ka laina B/Victoria. Ma waena o nā hihia i hoʻopaʻa ʻia o ka maʻi maʻi maʻi ma 15 mau ʻāina ma ka ʻāina ʻo ʻAsia-Pākīpika i kēlā me kēia mahina, ʻo ka helu o ka maʻi influenza B ʻo 0-92%[3]. ʻAʻole like me ka maʻi maʻi influenza A, hiki i nā hui kikoʻī e like me nā keiki a me ka poʻe ʻelemakule ke maʻalahi i ka maʻi maʻi influenza B a hiki ke hoʻopilikia i nā pilikia, kahi e kau ai i ke kaumaha nui ma mua o ka maʻi influenza A.[4].
Kanal
| FAM | ʻakika nucleic MP |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | ʻOropharyngeal swab sample |
| Ct | Flu A, Flu BCt≤35 |
| CV | <5.0% |
| LoD | Flu A a me Flu Bhe 200 kope/mL |
| kikoo | Cross-reactivity: ʻAʻohe hopena kea ma waena o ka pahu a me Bocavirus, rhinovirus, cytomegalovirus, respiratory syncytial virus, parainfluenza virus, Epstein-Barr virus, herpes simplex virus, Varicella-zoster virus, mumps virus, enterovirus, measles virus, metapneumovirus kanaka, adenovirus, coronavirus kanaka, coronavirus rotavirus, MERSrovirus, coronavirus novel, ʻaʻohe coronavirus hou. pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis carinii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Neisseria gonorrhoeas, Candida glauces, Candida glauces Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium, a me DNA genomic kanaka. Ka ho'āʻo hoʻopilikia: E koho i ka mucin (60 mg / mL), ke koko kanaka (50%), phenylephrine (2mg / mL), oxymetazoline (2mg / mL), sodium chloride (20mg / mL) me 5% preservative, beclomethasone (20mg / mL), dexamethasone (20mg / mL), a me ka flunisocinide (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mucinpirocin (20mg/mL), mucinpirocin (20mg/l. oseltamivir (60ng/mL), ribavirin (10mg/L) no nā ho'āʻo hoʻopilikia, a me nā hopena e hōʻike ana ʻaʻole e hoʻopilikia i ka ʻike ʻana o ka kit nā mea hoʻopili i nā mea i luna. |
| Nā mea hana pili | SLAN-96P Pūnaehana PCR Manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli QuantStudio®5 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.ua paipai ʻia no ka hōʻiliʻili ʻana a me kapono nā ʻanuʻu ma hopeconduce like me ka IFUo ka Kit.
