Influenza A Virus Universal/H1/H3
inoa huahana
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
Epidemiology
ʻO ka maʻi maʻi influenza kekahi ʻano ʻano o Orthomyxoviridae. He pathogen e hoʻoweliweli koʻikoʻi i ke olakino kanaka. Hiki iā ia ke hoʻopili nui i ka mea hoʻokipa. Hoʻopilikia ka maʻi ahulau ma kahi o 600 miliona mau kānaka ma ka honua holoʻokoʻa a he 250,000 ~ 500,000 ka make, ʻo ia ka maʻi influenza A ke kumu nui o ka maʻi a me ka make. ʻO ka maʻi Influenza A kahi RNA kaulaʻi ʻole i hoʻopaʻa ʻia. Wahi a kona hemagglutinin ili (HA) a me neuraminidase (NA), hiki ke hoʻokaʻawale ʻia ʻo HA i 16 subtypes, NA Māhele ʻia i 9 subtypes. Ma waena o nā maʻi maʻi influenza A, ʻo nā ʻano ʻano o nā maʻi maʻi maʻi influenza hiki ke hoʻopili pololei i ke kanaka: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 a me H10N8. Ma waena o lākou, ʻo nā subtypes H1 a me H3 he pathogenic nui, a he mea kūpono hoʻi e nānā.
Kanal
| FAM | influenza He ʻano virus nucleic acid |
| VIC/HEX | influenza A H1 type virus nucleic acid |
| ROX | influenza A H3 type virus nucleic acid |
| CY5 | hoʻomalu kūloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kope/μL |
| kikoo | ʻAʻohe ʻano hana ʻē aʻe me nā laʻana hanu ʻē aʻe e like me ka Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumovirus A1/B2, Coronavirus Respiratory Syncytial Virus. 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. a me DNA genomic kanaka. |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR Manawa maoli SLAN-96P Pūnaehana PCR manawa maoli(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻokele Thermal Cycler Manawa maoli (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Nucleic Acid Extraction a Purification Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd. Pono e hoʻokō pono ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana. ʻO 140μL ka nui o ka laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 60μL.
Koho 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Pono e hoʻokō pono ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana. ʻO 200μL ka nui o ka laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 80μL.
