ʻO ka maʻi Influenza A Universal/H1/H3
Inoa huahana
Pahu ʻike ʻike Nucleic Acid Multiplex HWTS-RT012 (Fluorescence PCR)
ʻAlapia
He ʻano ʻokoʻa ka maʻi influenza o Orthomyxoviridae. He pathogen ia e hoʻoweliweli nui ana i ke olakino kanaka. Hiki iā ia ke hoʻopili nui i ka mea hoʻokipa. Hoʻopilikia ka maʻi ahulau kau i kahi 600 miliona mau kānaka ma ka honua holoʻokoʻa a hoʻoulu i ka make ʻana o 250,000 ~ 500,000, ʻo ia ka maʻi influenza A ke kumu nui o ka maʻi a me ka make. ʻO ka maʻi influenza A he RNA kaula hoʻokahi. Wahi a kona hemagglutinin (HA) a me neuraminidase (NA), hiki ke hoʻokaʻawale ʻia ʻo HA i 16 mau ʻano, NA i hoʻokaʻawale ʻia i 9 mau ʻano. Ma waena o nā virus influenza A, ʻo nā ʻano o nā virus influenza e hiki ke hoʻopili pololei i nā kānaka: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 a me H10N8. Ma waena o lākou, he pathogenic loa nā ʻano H1 a me H3, a he mea kūpono loa ia e nānā ʻia.
Kanal
| ʻOhana | maʻi influenza A ʻano honua nucleic acid |
| VIC/HEX | maʻi influenza A H1 ʻano nucleic acid |
| ROX | maʻi influenza A ʻano H3 waikawa nucleic |
| CY5 | ka mana kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 9 mahina |
| ʻAno Laʻana | ʻūpā nasopharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kope/μL |
| Kūlana kikoʻī | ʻAʻohe cross-reactivity me nā hāpana hanu ʻē aʻe e like me Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. a me ka DNA genomic kanaka. |
| Nā Mea Hana Pili | ʻŌnaehana PCR Manawa Maoli Biosystems 7500 i hoʻopili ʻia Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli QuantStudio®5 Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.) ʻŌnaehana PCR Manawa Maoli LightCycler®480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻopololei Wela Manawa Maoli (Suzhou Molarray Co., Ltd.) ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
ʻO ka Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd. Pono e hoʻokō pono ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka laʻana i unuhi ʻia he 140μL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 60μL.
Koho 2.
ʻO ka Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Pono e hoʻokō pono ʻia ka unuhi ʻana e like me nā kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka laʻana i unuhi ʻia he 200μL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80μL.
