ʻO ka maʻi hanu o ka Hikina Waena Coronavirus Nucleic Acid
Inoa huahana
HWTS-RT031A-Ka Pahu ʻIke ʻAkika Nukleika no ka Maʻi Haʻihaʻi Hikina Waena (PCR Fluorescent)
ʻAlapia
ʻO ka maʻi coronavirus hanu Hikina Waena (MERS-CoV), he β-coronavirus e hoʻoulu aiʻO ka maʻi hanu i loko o ke kanaka, ua ʻike mua ʻia i loko o kahi maʻi kāne Saudi Arabia he 60 ona makahiki i make ma ka lā 24 o Iulai, 2012. ʻO ka hōʻike lapaʻau o ka maʻi MERS-CoV mai ke ʻano asymptomatic a i ʻole nā hōʻailona hanu ʻoluʻolu a hiki i ka maʻi hanu koʻikoʻi a hiki i ka make.
Kanal
| ʻOhana | RNA o ka maʻi MERS |
| VIC(HEX) | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18 ℃ I ka pōʻeleʻele |
| Ola papa | 9 mahina |
| ʻAno Laʻana | Nā ʻōpala nasopharyngeal i hōʻiliʻili hou ʻia |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1000 Kope/mL |
| Kūlana kikoʻī | ʻAʻohe cross-reactivity me nā coronavirus kanaka SARSr-CoV a me nā pathogens maʻamau ʻē aʻe. |
| Nā Mea Hana Pili: | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli QuantStudio®5 Nā ʻōnaehana PCR Manawa Maoli SLAN-96P ʻŌnaehana PCR Manawa Maoli LightCycler®480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Koho 1.
ʻO ka mea hoʻohuihui i ʻōlelo ʻia: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd.
Koho 2.
ʻO ka mea hoʻoheheʻe i ʻōlelo ʻia: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
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