ʻO ka waikawa nucleic type o ka maʻi Monkeypox

Kiʻi i hōʻike ʻia o ka maʻi Monkeypox Nucleic Acid

Wehewehe Pōkole:

Hoʻohana ʻia kēia pahu hana no ka ʻike ʻana i ka qualitative o ka maʻi monkeypox clade I, clade II nucleic acids i loko o ka wai ʻili kanaka, serum a me nā laʻana swab oropharyngeal.

E leka uila mai iā mākou

Nā kikoʻī huahana

Nā Lepili Huahana

Inoa huahana

HWTS-OT201Pahu ʻike waikawa nucleic no ka maʻi Monkeypox(PCR fluorescence)

ʻAlapia

ʻO ka Monkeypox (Mpox) kahi maʻi lele zoonotic koʻikoʻi i hoʻokumu ʻia e ka Monkeypox Virus (MPXV). He ʻano poepoe a oval paha ka MPXV, a he maʻi DNA kaula pālua me ka lōʻihi o kahi o 197Kb. Hoʻolaha nui ʻia ka maʻi e nā holoholona, a hiki i nā kānaka ke loaʻa i ka maʻi ma ke nahu ʻia e nā holoholona i loaʻa i ka maʻi a i ʻole ma ka pili pololei ʻana me ke koko, nā wai kino a me ka ʻulaʻula o nā holoholona i loaʻa i ka maʻi. Hiki ke hoʻolaha ʻia ka maʻi ma waena o nā kānaka, ma o nā kulu hanu i ka wā o ka launa pū ʻana me ke alo a i ʻole ma o ka pili pololei ʻana me nā wai kino o ka mea maʻi a i ʻole nā mea i haumia. Ua hōʻike ʻia nā haʻawina ua hana ʻo MPXV i ʻelua mau clades like ʻole: clade I (i ʻike mua ʻia ʻo ka Central African clade a i ʻole Congo Basin clade) a me clade II (i kapa ʻia ʻo West African clade). Ua hōʻike maopopo ʻia ka mpox o ka Congo Basin clade he hiki ke hoʻolaha ma waena o nā kānaka a hiki ke make, ʻoiai ʻo ka mpox o ka West African clade e hoʻoulu i nā hōʻailona māmā a he haʻahaʻa ka nui o ka laha ʻana mai ke kanaka a i ke kanaka.

ʻAʻole i manaʻo ʻia nā hopena hoʻāʻo o kēia pahu e lilo i mea hōʻailona hoʻokahi no ka ʻike ʻana i ka maʻi MPXV i nā maʻi, pono e hui pū ʻia me nā ʻano lapaʻau o ka mea maʻi a me nā ʻikepili hoʻāʻo hale hana ʻē aʻe e hoʻoholo pololei i ka maʻi o ka pathogen a hoʻokumu i kahi hoʻolālā lapaʻau kūpono e hōʻoia i ka mālama palekana a me ka maikaʻi.

Nā Palena ʻenehana

ʻAno Laʻana	wai ʻili kanaka, swab oropharyngeal a me serum
Ct	38
ʻOhana	ʻĀpana FAM-MPXV II VIC/HEX-MPXV ʻĀpana I
CV	≤5.0%
LoD	200 Kope/μL
Kūlana kikoʻī	E hoʻohana i ka pahu hana e ʻike i nā maʻi ʻē aʻe, e like me ka maʻi Smallpox, ka maʻi Cowpox, ka maʻi Vaccinia, HSV1, HSV2, Human Herpesvirus ʻano 6, Human Herpesvirus ʻano 7, Human Herpesvirus ʻano 8, Measels vieus, Chicken pox-Herpes zoster virus, EB virus, Rubella virus etc., a me ʻaʻohe hopena kea.
Nā Mea Hana Pili	Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli QuantStudio^®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P Nā ʻōnaehana ʻike PCR manawa maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli BioRad CFX96 ʻO BioRad CFX Opus 96 Nā ʻōnaehana PCR Manawa Maoli