● ʻO ka Mycobacterium Tuberculosis
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ʻO ka Mycobacterium Tuberculosis Nucleic Acid a me Rifampicin (RIF), Resistance (INH)
Hoʻohana ʻia kēia pahu no ka ʻike ʻana i ka DNA Mycobacterium tuberculosis in vitro i loko o ka sputum kanaka, ka moʻomeheu paʻa (LJ Medium) a me ka moʻomeheu wai (MGIT Medium), ka wai lavage bronchial, a me nā mutations ma ka ʻāpana codon amino acid 507-533 (81bp, rifampicin resistance determing region) o ka gene rpoB o Mycobacterium tuberculosis rifampicin resistance, a me nā mutations ma nā wahi mutation nui o Mycobacterium tuberculosis isoniazid resistance. Hāʻawi ia i ke kōkua i ka ʻike ʻana i ka maʻi Mycobacterium tuberculosis, a ʻike ia i nā genes pale nui o rifampicin a me isoniazid, e kōkua ana i ka hoʻomaopopo ʻana i ke kūʻē ʻana o ka lāʻau lapaʻau o Mycobacterium tuberculosis i loaʻa i ka maʻi.
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Ka Hoʻololi ʻana o ka Mycobacterium Tuberculosis INH
He kūpono kēia pahu no ka ʻike ʻana i nā wahi mutation nui i nā laʻana sputum kanaka i hōʻiliʻili ʻia mai nā poʻe maʻi Tubercle bacillus maikaʻi e alakaʻi ai i ka mycobacterium tuberculosis INH: ʻĀpana hoʻolaha InhA -15C>T, -8T>A, -8T>C; ʻĀpana hoʻolaha AhpC -12C>T, -6G>A; mutation homozygous o KatG 315 codon 315G>A, 315G>C.
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Ke kū'ē ʻana i ka Mycobacterium Tuberculosis Rifampicin
He kūpono kēia pahu hana no ka ʻike ʻana i ka qualitative o ka homozygous mutation ma ka ʻāpana codon amino acid 507-533 o ka gene rpoB e hoʻoulu ai i ke kūʻē ʻana o Mycobacterium tuberculosis rifampicin.
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ʻO ke kū'ē ʻana o ka Mycobacterium Tuberculosis Nucleic Acid a me Rifampicin
He kūpono kēia pahu hana no ka ʻike ʻana i ka DNA Mycobacterium tuberculosis i loko o nā hāpana sputum kanaka ma vitro, a me ka homozygous mutation ma ka ʻāpana codon amino acid 507-533 o ka gene rpoB e hoʻoulu ai i ke kūʻē ʻana o Mycobacterium tuberculosis rifampicin.
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ʻO ka DNA o ka Mycobacterium Tuberculosis
He kūpono ia no ka ʻike ʻana i ka Mycobacterium tuberculosis DNA i loko o nā hāpana sputum lapaʻau kanaka, a he kūpono no ka ʻike kōkua o ka maʻi Mycobacterium tuberculosis.
